搜索结果: 1-15 共查到“知识库 农学 PCR-RFLP”相关记录51条 . 查询时间(0.062 秒)
A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses
MyoD genes muscle fibres pig
2015/6/5
Muscle fibre formation takes place during embryonic development and is regulated by the MyoD gene family, which consists of four genes, MYOD1, myogenin, MYF5 and MRF4. A relationship was studied betwe...
A DdeI PCR-RFLP detecting a novel missense mutation of the POU1F1 gene showed no effects on growth traits in cattle
bovine POU1F1 gene PCR-RFLP growth traits
2015/6/5
In this study, a novel missense (NM_174579:c.1201C>T) mutation in exon 6 at the bovine POU1F1 locus is reported, which results in p.S284F, namely, Ser (TCT) > Phe (TTT) at position 284 of the mature p...
Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis
Pork Contamination Detection Meatballs Polymerase Chain Reaction
2016/5/17
This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets...
本研究旨在建立猪瘟病毒野毒株和兔化弱毒疫苗株RTPCRRFLP鉴别检测方法。根据Shimen株设计1对特异性引物，建立猪瘟病毒 RTPCRRFLP检测方法；对20份疑似猪瘟临床样品进行检测，并对检出的山东8株流行野毒株和2株疫苗株PCR产物进行克隆与序列分析，验证上述方法。结果RTPCR扩增片段为825 bp，产物经RFLP分析，野毒株的PCR产物能被ApaⅠ酶切为322 bp和503 ...
本研究旨在建立猪瘟病毒野毒株和兔化弱毒疫苗株RT-PCR-RFLP鉴别检测方法。根据Shimen株设计1对特异性引物，建立猪瘟病毒 RT-PCR-RFLP检测方法；对20份疑似猪瘟临床样品进行检测，并对检出的山东8株流行野毒株和2株疫苗株PCR产物进行克隆与序列分析，验证上述方法。结果RT-PCR扩增片段为825 bp，产物经RFLP分析，野毒株的PCR产物能被ApaⅠ酶切为322 bp和503 ...
琅琊鸡Mx基因3’端部分序列PCRRFLP与序列分析
琅琊鸡 Mx基因 PCRRFLP 序列分析
2010/1/8
［目的］为琅琊鸡分子标记辅助选择、抗病育种提供依据。［方法］利用PCRRFLP技术对琅琊鸡Mx基因3’端片段的Hae Ⅲ的酶切多态性进行分析。［结果］琅琊鸡群体中存在Mx基因Hae Ⅲ酶切位点的多态性，且均有A、B 2个等位基因，基因频率分别为0.562和0.438。Mx基因Hae Ⅲ酶切位点的基因型分布在琅琊鸡群体中极显著偏离HardyWeinberg平衡状态(...
PCRRFLP技术检测嘉兴凤桥种猪氟烷基因
凤桥 猪 氟烷基因 PCR RFLP
2009/9/16
［目的］ 为凤桥镇通过合理利用氟烷基因提高猪肉品质奠定理论基础。［方法］ 从嘉兴县凤桥镇的规模化养猪场随机抽取种猪血样，通过PCRRFLP技术进行氟烷基因检测。［结果］ HhaⅠ内切酶的识别位点为5′GCG↓C3′。PCR扩增产物是含RYR1基因第1 843位点长度为659 bp的片段。如果猪的基因型为NN，由于未发生胞嘧啶C到胸腺嘧啶T的突变，可以看到2条带。如果猪的基因型为nn，由...
Discrimination among Japanese Species of the Orius Flower Bugs (Heteroptera: Anthocoridae) Based on PCR-RFLP of the Nuclear and Mitochondrial DNAs
natural enemy predatory bugs species identification internal transcribed spacer 1 cytochrome oxidase subunit I
2009/9/9
A PCR-RFLP-based method of species identification was considered for 5 Japanese species of Orius Wolff flower bugs, Orius strigicollis (Poppius), O. minutus (Linnaeus), O. sauteri (Poppius), O. nagaii...
[ 目的] 提高鸡的产蛋性能和育种效率。[ 方法] 利用一个已知的鸡OVR 基因PCR- RFLP 位点, 对乌骨鸡、土乌鸡、青脚麻鸡3 个群
体进行鸡OVR 基因PCR- RFLP 位点多态性检测, 并且分析该RFLPS 与一定时期内产蛋数和产蛋总重的关系。[ 结果] 结果表明, 在上述
群体中均存在RFLPS ; 不同基因型间的产蛋数、产蛋总重差异不明显。[ 结论] RFLP 广泛存在于不...
本研究采用PCR-RFLP方法，对莱芜黑猪、鲁莱黑猪、里岔黑猪、鲁烟白猪、新沂蒙黑猪5个山东本地猪种和大约克夏、长白、杜洛克3个引进猪种共八个猪种323头繁殖母猪进行PRLR和RBP4基因的多态性检测，并对其遗传多样性进行分析。结果表明：两个基因位点在八个猪种的测定群体中均存在多态性，在三种基因型中AB基因型出现频率最高，山东本地猪种与引进猪种间基因型频率存在显著差异（P<0.05）。八个猪种PR...
Detection and Characterization of Plum Pox Potyvirus（PPV）by DAS-ELISA and RT-PCR / RFLP Analysis in Turkey
Enzyme-linked immunosorbent assay plum pox potyvirus restriction analysis reverse transcription-polymerase chain reaction
2009/1/13
This study was conducted to determine the presence of plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterize PPV isolates by serological and mol...
南江黄羊GH基因的PCR-RFLP与早期生长发育的相关分析
南江黄羊GH基因 PCR-RFLP 生长发育 相关分析
2008/12/1
生长激素（GH）是一种调节动物生长的重要激素，具有广泛的生理功能。本试验以90只南江黄羊母羊为研究材料，对山羊GH基因部分片段进行了PCR-RFLP分析，并对南江黄羊GH基因的PCR-RFLP与早期生长发育性状进行了相关研究。结果表明扩增产物片段经序列测定长度为985bp，包括gGH内含子3、内含子4以及外显子3、外显子4和外显子5的全部序列。经限制性内切酶AciⅠ和FokⅠ酶切，均发现在南江黄羊...