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Covalent Self-labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings
BY-2 cells Arabidopsis SNAP-tag live cell imaging microtubules endocytosis PIN2 auxin transporter
2023/12/21
Synthetic chemical fluorescent dyes promise to be useful for many applications in biology. Covalent, targeted labeling, such as with a SNAP-tag, uses synthetic dyes to label specific proteins in vivo ...
Protein kinase sensors: an overview of new designs for visualizing kinase dynamics in single plant cells
plant cells protein kinase sensors kinase dynamics signaling networks SnRK2
2023/12/1
Protein kinase dynamics play key roles in regulation of cell differentiation, growth, development and in diverse cell signaling networks. Protein kinase sensors enable visualization of protein kinase ...
Cross-talk between clathrin-dependent post-Golgi trafficking and clathrin-mediated endocytosis in Arabidopsis root cells
Adaptor protein Arabidopsis TPLATE clathrin endocytosis exocytosis
2023/12/1
Coupling of post-Golgi and endocytic membrane transport ensures that the flow of materials to/from the plasma membrane (PM) is properly balanced. The mechanisms underlying the coordinated trafficking ...
Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells
Single-cell transcriptomics photosynthetic Arabidopsis thaliana high-throughput transcriptome metabolic stress-related
2023/11/30
As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization of leaf mesophyll and epidermal cell layers orchestrates photosynthesis an...
Simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived from umbilical cord blood
生物反应器 UCB MSCs RWV GCSC
2023/3/1
The simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived from umbilical cord blood were carried out using bioreactors. The co-culture of umbilical cord blo...
SARS-CoV-2-encoded nucleocapsid protein acts as a viral suppressor of RNA interference in cells
SARS-CoV-2-encoded nucleocapsid protein acts viral suppressor of RNA interference cells
2020/6/12
We thank Prof. Peng Zhou (Wuhan, China) for kindly providing materials and thank Yan Wu and Weijuan Shang for experimental assistance. This work was supported by the Strategic Priority Research Progra...
Effects of selenium on selenoprotein synthesis and antioxidant parameters of bovine mammary epithelial cells
trace element dairy cows glutathione peroxidase thioredoxin reductase antioxidant function
2018/11/23
This study aimed to investigate the effects of selenium (Se) on the selenoproteins synthesis and antioxidant parameters of bovine mammary epithelial cells (BMECs). The experiment was conducted as a si...
DEEP CONVOLUTIONAL NEURAL NETWORK FOR AUTOMATIC DETECTION OF DAMAGED PHOTOVOLTAIC CELLS
UAV, monitoring/inspection Anomaly Detection Deep Learning Photovoltaic Panels Thermography Classification
2018/6/4
The number of distributed Photovoltaic (PV) plants that produce electricity has been significantly increased, and issue of monitoring and maintaining a PV plant has become of great importance and invo...
Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Selenium
selenomethionine selenoprotein synthesis arachidonic acid metabolism mitogen-activated protein kinase
2018/3/5
The uncontrolled release of arachidonic acid (ARA) and its metabolism by lipoxygenase (LOX) pathway can induce
and aggravate cellular oxidative stress. Selenium (Se) is an integral part of some antio...
Loss of intercalated cells (ITCs) in the mouse amygdala of Tshz1 mutants correlates with fear, depression and social interaction phenotypes
brain development telencephalon transcription factor
2018/3/5
The intercalated cells (ITCs) of the amygdala have been shown to be critical regulatory components of amygdalar circuits, which control appropriate fear responses. Despite this, the molecular processe...
Retinoic acid attenuates oxidative injury in bovine mammary epithelial cells induced by hydrogen peroxide
vitamin A thioredoxin reductase oxidative injury
2017/12/25
The objective of this study was to explore how retinoic acid (RA) attenuates oxidative injury induced by hydrogen peroxide (H2O2) in bovine mammary epithelial cells (BMEC). Subconfluence BMEC were ran...
Retinoic Acid Attenuates Oxidative Injury in Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide
vitamin A thioredoxin reductase oxidative injury arachidonic acid p38 mitogen-activated protein kinase
2018/3/5
Jin L., Yan S., Shi B., Shi H., Guo X., Li J. (2017): Retinoic acid attenuates oxidative injury in bovine
mammary epithelial cells induced by hydrogen peroxide. Czech J. Anim. Sci., 62, 539–548.
The...
Lignosulfonic acid promotes hypertrophy in 3T3-L1 cells without increasing lipid content and increases their 2-deoxyglucose uptake
Adipogenesis Diabetes Dietary Fiber Glucose Uptake Lignosulfonic Acid
2017/3/7
Adipose tissue plays a key role in the development of obesity and diabetes. We previously reported that lignosulfonic acid suppresses the rise in blood glucose levels through the inhibition of α-gluco...
Transcriptome profile of one-month-old lambs’ granulosa cells after superstimulation
Lamb Granulosa Cells Sheep RNA Seq Superstimulation
2017/3/7
Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte m...
Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells
mode of existence disinfection fluorescence microscopy
2016/7/6
Epifluorescence microscopy (EFM) was used to study the biofilm formation of Pseudomonas aeruginosa after 6, 24, 30, 48, 54, 72, 78, and 96 h growth in a chamber slide system. For this purpose, the bio...