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Purification and Gene Cloning of Catalase from Staphylococcus warneri ISK-1
catalase Staphylococcus purification
2009/7/8
To investigate the possibility of applying staphylococcal catalase to eliminate H2O2 in lactic acid fermentation, an intracellular catalase from Staphylococcus warneri ISK-1 which exhibited high catal...
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白（β-lactoglobulin,BLG）基因，扩增出的两个片段分别克隆到T载体上，利用BLG基因序列自身存在的NarI单酶切位点进行拼接，获得了全长为7.2kb的山羊BLG基因克隆，并构建了它的真核表达载体，经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠，经PCR和Southern印迹分析...
摘要本文简要介绍简并PCR技术，包括什么是简并引物，如何设计简并引物，进行简并PCR的反应条件，应用简并PCR获得全长基因的方法和简并PCR技术的应用范围，并对简并PCR技术的局限性及其新进展进行讨论。在此基础上，简述基因的克隆策略以及简并PCR技术在基因克隆中的应用。简并PCR技术是寻找和发现“新”基因或蛋白质家族新成员的一种非常有用的工具。
Abstract:Degenerate PCR i...
图位克隆技术在分离植物基因中的应用 The Application of Map-based Gene Cloning Method on Isolating Genes of Plants
图位克隆 基因定位 物理图谱 比较作图
2007/12/7
摘要本文综述图位克隆技术的原理及基本技术环节,并与其它基因克隆技术相比较说明图位克隆技术的优缺点。本文还对近年来图位克隆技术在分离不同的植物发育基因上的广泛应用进行了简要概述,并对其应用前景和近期的预期进展作出展望。
Abstract:In this review,the principle and basis steps of map-based gene cloning on plant w...
Gene Cloning of 30 kDa Toxoplasma gondii Tachyzoites Surface An¬tigen (SAG1)
Toxoplasma tachyzoite SAG1 pQE-30 expression vector pTZ57R cloning vector
2010/4/12
Background: Toxoplasma gondii is an obligate intracellular parasite and its sexual and asexual cycles, respectively take place in the intestinal epithelial cell of definitive host and tissue of interm...
Gene Cloning and Nucleotide Sequence of Human Dihydrolipoamide Dehydrogenase-Binding Protein
Pyruvate Dehydrogenase Complex Dihydrolipoamide Dehydrogenase-Binding Protein Nucleotide Sequence
2016/4/8
The pyruvate dehydrogenase complex (PDC), a member of -keto acid dehydrogenase complex, catalyzes the oxidative decarboxylation of pyruvate with the formation of CO2, acetyl-CoA, NADH, and H+....