搜索结果: 1-15 共查到“知识库 Calpain”相关记录15条 . 查询时间(0.072 秒)
本发明公开了一种基于μ-ｃａｌｐａｉｎ活性判断肌肉最佳成熟度的方法，牲畜肉嫩度用肌原纤维小片化指数代表，持水性用加压失水率代表，包括：步骤一、牲畜宰后取多份生肉样品，置于温度２-６℃下分别成熟不同时间；步骤二、测定成熟不同时间下的各份生肉样品中的μ-ｃａｌｐａｉｎ相对活性、肌原纤维小片化指数及加压失水率；步骤三、绘制成熟时间与μ-ｃａｌｐａｉｎ相对活性、成熟时间与加压失水率的趋势图，拟合成熟时间与...
探究microRNA（miR）-124-3p作用于calpain 1抑制氧化应激对脊髓损伤后神经元凋亡的影响及其分子机制。方法：选取成年雄性SD大鼠40只，随机分成A组（miR-124-3p agomir组）、B组（agomir-NC组）、C组（model组）以及D组（sham组），每组10只。构建脊髓损伤模型前1d，采用改良的Sloane法向大鼠的脊髓组织T10段蛛网膜下腔注射miR-124-3...
探讨大鼠肝纤维化过程中钙激活中性蛋白酶2（calpain 2）与半胱氨酸天冬氨酸酶3（caspase-3）表达变化及其在肝纤维化发生发展中作用。方法 雄性Wistar大鼠40只，随机分为对照4、8周组和肝纤维化模型4、8周组，每组各10只；肝纤维化组按0.3 mL/100g皮下注射40% CCl4植物油溶液，每隔3 d注射1次，对照组给予等量植物油；测定肝脏指数、血清谷丙转氨酶（ALT）及谷草转氨...
评价calpain抑制剂ALLN对酵母多糖足底炎性疼痛模型大鼠的镇痛作用及其对脊髓背角环氧化酶-2（COX-2）表达水平的影响，探讨calpain在炎性疼痛中的作用机制。方法 SD大鼠48只，随机分为对照组、假手术组和酵母多糖组，按Meller方法制作酵母多糖足底炎性疼痛模型。分别于制模前和制模后0.5、1、2、4、8、24和48 h测定各组大鼠左侧后足机械刺激缩足阈值（MWT）和左侧后足最大厚度...
The Effects of Different Chilling Methods on Meat Quality and Calpain Activity of Pork Muscle Longissimus Dorsi
calpain activity, carcasses, chilling methods, meat quality, pork
2012/8/14
The objective of this study was to investigate the effects of conventional chilling (0 to 4 ◦C), rapid chilling (RC, –20 ◦C for 30 min, followed by 0 to 4 ◦C), and short-duration chi...
Association of Polymorphisms in the Calpain I Gene with Meat Quality Traits in Yanbian Yellow Cattle of China
Yanbian Yellow Cattle CAPNI Meat Quality SNPs
2016/5/5
The calpain I (CAPN1) gene is an important marker for meat tenderness and marbling score in the bovine, but there were no studies to determine whether the CAPN1 gene had an association with other meat...
Calpain与心肌细胞凋亡
卡配因 心肌细胞 细胞凋亡
2010/3/31
Cardiomyocytes apoptosis plays a crucial role in the pathologic process of some ischemic and non-ischemic heart diseases. Since the discovery of the calpain family, more and more studies have demonstr...
高糖下调大鼠许旺细胞calpainⅡ的表达
许旺细胞 葡萄糖 卡配因Ⅱ
2009/8/26
目的：观察高糖对体外培养的大鼠许旺细胞calpainⅡ表达的影响。 方法： 体外培养大鼠许旺细胞，用MTT检测许旺细胞的存活力，用原位杂交和免疫细胞化学方法分别测定许旺细胞calpainⅡmRNA和蛋白的表达。 结果： 高糖（50 mmol/L）培养24 h，许旺细胞存活力明显低于对照组（30 mmol/L）（73.95% vs 100%， P＜0.01）。许旺细胞calpainⅡ mRNA阳性颗...
Calpain mu与肝脏的缺血再灌注损伤
Calpain mu 肝脏 缺血再灌注损伤
2009/7/28
随着医学科学的迅速发展，肝移植和肝癌手术已逐渐成为普遍的手术. 然而，供肝的保存和肝癌手术时，肝门部常温阻血后的再灌注损伤是导致移植肝脏和肝癌术后的肝脏功能不良的重要因素. 当肝脏的缺血再灌注时，Ca2+细胞内大量流入，引起与Ca2+依赖性水解蛋白酶(calpain mu)的活性化(初期反应变化)为十分重要的基点. 我们曾经通过TBHP(tert-butyl hydroperxride)处理培...
calpain 及其基因在肉质性状育种中的研究进展
clpain 肉嫩度 calpain 基因 多态性
2009/4/10
综述了calpain 的结构形式及其作用的一般机制, 并介绍了关于calpain 基因在部分畜禽品种中的遗传变异及相关的研究进展。
Calpain and calpastatin are located between the plasma membrane and outer acrosomal membrane of cynomolgus macaque spermatozoa
Calpain calpastatin plasma membrane outer acrosomal membrane cynomolgus macaque spermatozoa
2008/12/25
Mammalian sperm must undergo an acrosome reaction prior to penetration of the zona pellucida and subsequent fusion with an oocyte. Sperm gain the capability to acrosome react after a period of capac...
μ-calpain激活因子UK114的克隆与序列分析
钙激活酶 UK114 克隆 序列分析
2008/4/3
构建了山羊肝脏 cDNA文库，采用平板裂解法提取噬菌体DNA，以此为模板用所设计的引物以PCR法扩增出μcalpain激活蛋白UK114基因，并克隆到pGEM-T easy载体。序列分析表明，UK114 cDNA包括起始密码子和终止密码子在内全长共计1 017 bp,5′非编码区长为39 bp，3′非编码区长为567 bp，编码区长411 bp，编码137个氨基酸序列。与GenBank中Colo...
Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system
Meat Tenderness Postmortem Calpain
2012/8/10
Tenderness has been repeatedly reported as the most important quality aspect of meat. However, a number of studies have shown that a significant portion of retail meat can be considered tough. As a co...
Comparative Studies on Metabolic Rate and Calpain/Calpastatin Activity between Hanwoo and Holstein Beef
Holstein Beef Temperature Conditioning Metabolic Rate Calpain Calpastatin
2016/4/12
The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and ca...
Effects of Dietary Treatment, Gender, and Implantation on Calpain/Calpastatin Activity and Meat Tenderness in Skeletal Muscle of Korean Native Cattle
Calpain Activity Meat Tenderness Feeding Pattern Korean Native Cattle
2016/4/12
The objectives of this study were to examine calpain activity and meat tenderness by three different feeding patterns in Korean native cattle (KNC). Total forty-five animals were assigned each fifteen...