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林志强/吕丹/游富平团队联合提出基于梯度pH敏感聚合物的抗病毒新策略(图)
pH 北京大学基础医学院 聚合物 包膜病毒
2023/8/14
2022年2月28日,北京大学基础医学院林志强/吕丹/游富平团队合作在《Advanced Materials》在线发表了题为“A gradient pH-sensitive polymer-based antiviral strategy via viroporin-induced membrane acidification”的研究论文,成功构建了一套pH敏感的聚合物探针,精确测定了被病毒感染的...
pH-磁双响应Janus笼
Janus笼 pH响应性 可控释放
2022/3/29
Effects of chilled storage and pH of activating solution on different motility parameters in burbot (Lota lota) sperm
short-term storage activation Lota lota sperm analysis CASA parameters
2018/11/23
The effects of a simple saline solution prepared using two different pH (4.4 and 8.5) on sperm motility in burbot were investigated. Results were recorded during a 96-hour chilled storage (4°C) in 24-...
目的 制备pH敏感电荷反转型姜黄素纳米粒子(PCE/Cur NPs),优化制备工艺并考察PCE/Cur NPs的理化性质及其对黑色素瘤(B16)细胞的抑制作用。方法 在正电性材料甲氧基聚乙二醇-聚己内酯-聚乙烯亚胺(MPEG-PCL-PEI,PCE)的基础上键合1,2-环己二酸酐使其变成负电性材料,制备带负电的PCE/Cur NPs,该纳米粒子具有pH值依赖性,pH值>7时,PCE/Cur NPs...
EGCG对β-淀粉样蛋白聚集的抑制作用:pH的影响
β-淀粉样蛋白 聚集 pH值 表没食子儿茶素没食子酸酯 抑制作用
2019/1/14
研究了表没食子儿茶素没食子酸酯(EGCG)在不同pH值(5.0,6.0和7.4)下对β-淀粉样蛋白(Aβ42)聚集的抑制作用.结果表明,虽然在上述pH范围内EGCG均可抑制Aβ42聚集和细胞毒性,但不同pH值下EGCG与Aβ42的作用方式不同.当pH=5.0时,Aβ42可在数秒内聚集,EGCG-Aβ42相互作用最弱,因此聚集前期EGCG不能有效抑制Aβ42纤维化;
通过耗散粒子动力学方法,研究了pH响应性嵌段聚合物(聚丙烯酸-聚-2-乙烯基吡啶PAA-P2VP)接枝在纳米孔内的开关效应. 探讨了嵌段序列Wall-P2VP-PAA和Wall-PAA-P2VP对响应性开关效应的影响,结果表明,只有Wall-PAA-P2VP嵌段序列才能在不同的环境下形成智能响应性开关. 接枝密度对智能响应性开关影响的研究表明,只有在合适的嵌段聚合物链接枝密度下,形成的孔道才能够达...
pH响应木质素基胶体球的制备和表征
季铵化碱木质素 十二烷基苯磺酸钠 胶体球 pH响应性
2019/1/31
以造纸制浆废液中的松木碱木质素(AL)为原料,通过季铵化改性,制备了季铵化碱木质素(QAL).QAL与十二烷基苯磺酸钠(SDBS)通过静电作用形成QAL/SDBS复配物,将QAL/SDBS复配物在乙醇/水混合溶剂中进行自组装得到具有pH响应性的胶体球.采用X射线光电子能谱(XPS)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、元素分析和静态接触角研究了胶体球的形成过程和结构.研究结果表明,
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.3.Temperature Dependence of Proton Transfer
Green Fluorescent Proteins Amino Acid Substitution Spectrometry, Fluorescence Kinetics Mutagenesis,Site-Directed Models,Theoretical Genetic Variation Hydrogen-Ion Concentration Thermodynamics Solvents
2016/5/24
In parts 1 and 2 of this series [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S. Y., Xi, L. X., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Bio...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.2.Excited-State Dynamics
Animals Protons Deuterium Luminescent Proteins Green Fluorescent Proteins Spectrometry, Fluorescence Biosensing Techniques Kinetics Genetic Variation Hydrogen-Ion Concentration
2016/5/24
In the preceding paper [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Biochemistry 41, 1...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.1.Structural Characterization and Preliminary Application
Animals Cricetulus Cell Line Threonine Luminescent Proteins Green Fluorescent Proteins Microscopy, Confocal Crystallography, X-Ray Transfection Amino Acid Substitution
2016/5/24
Novel dual emission, pH-sensitive variants of the green fluorescent protein (GFP) have been constructed and are suitable for ratiometric emission measurements in vivo. This new class of GFPs, termed d...
Are Ph.D. Students Able to Explore Career Paths That Their Advisors Disparage?
Career Paths Advisors Disparage
2015/7/31
This essay is written for a particular group of Ph.D. students in oceanography, not the majority who are in the Ph.D. program to learn how to be researchers in research universities and research insti...
In 1896, Arrhenius provided the first roughly quantitative sense of the plausible magnitude of human-induced changes in the concentration of CO2 in the atmosphere. Since then, all chemists could be aw...
Acid-base homeostasis of blood and pH of abomasum in calves fed non-acidified and acidified milk replacer
calves milk feeding formic acid acid-base balance pH of abomasums
2015/6/4
Experiments were conducted on calves divided into three groups, 6 animals in each, to determine the influence of the intake of non-acidified and acidified milk replacer on the level of acid-base homeo...
Development of a Cytosolic pH Reporter for Tobacco BY2 Cells
Tobacco BY2 Cells Cytosolic pH Reporter
2015/1/6
The regulation of pH is a critical homeostatic function of plant cells. In addition to acting as the primary cationic species responsible for energizing the plasma membrane, protons likely act as an i...