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Changes in Membrane Lipid Order With Capacitation in Rhesus Macaque（Macaca mulatta）Spermatozoa
Primate sperm merocyanine 540 annexin-V tyrosine phosphorylation
2008/12/24
Lipophilic fluorescent dye merocyanine 540 is believed to stain cell membranes with increasing affinity as the lipid components become more disordered and has been associated with changes in m...
The Proacrosin Binding Protein, sp32, Is Tyrosine Phosphorylated During Capacitation of Pig Sperm
Fertilization p32 acrosin acrosome reaction
2008/12/23
Mammalian sperm must undergo capacitation, a preparation period in the female reproductive tract or in vitro, in order to fertilize. We have previously described a Mr 32 000 tyrosine phosphorylated ...
Annexin V Binding and Merocyanine Staining Fail to Detect Human Sperm Capacitation
FACScan membrane architecture phosphatidylserine translocation
2008/12/22
The signaling pathways that characterize the process of capacitation of human spermatozoa are still largely unknown. Modifications in the lipid architecture of the sperm plasma membrane have been de...
Sperm Membrane Dynamics Assessed by Changes in Lectin Fluorescence Before and After Capacitation
Fertilization gametes membrane domain protein mobility sperm activation
2008/12/22
Sperm capacitation is correlated with acquisition of fertilizing ability, and the molecular events underlying this process are only beginning to be understood. A number of membrane changes associate...
Inhibitors of Phosphoinositide 3-Kinase, LY294002 and Wortmannin, Affect Sperm Capacitation and Associated Phosphorylation of Proteins Differently：Ca2+-Dependent Divergences
Spermatozoa signal transduction Akt
2008/12/22
Sperm capacitation is regulated by multiple pathways that also control sperm motility and tyrosine (Tyr) phosphorylation of several sperm proteins. Among the reported pathways, phosphoinositide 3-ki...
The Importance of Calcium in the Appearance of p32，a Boar Sperm Tyrosine Phosphoprotein，During In Vitro Capacitation
Tyrosine phosphorylation chelator A23187 indo-1
2008/12/19
After ejaculation, mammalian sperm must undergo a preparation period known as "capacitation" to become capable of fertilizing the oocyte. Although physiological capacitation occurs in the female g...
Progesterone Receptor Availability in Mouse Spermatozoa During Epididymal Transit and Capacitation：Ligand Blot Detection of Progesterone-Binding Protein
Acrosome reaction sperm maturation
2008/12/19
The goals of the present study were to determine the availability of progesterone (P4) receptor (P4r) in mouse sperm during maturation and capacitation and to make the first steps toward a character...
Progesterone Receptor Availability in Mouse Spermatozoa During Epididymal Transit and Capacitation：Ligand Blot Detection of Progesterone-Binding Protein
Acrosome reaction sperm maturation
2008/12/19
The goals of the present study were to determine the availability of progesterone (P4) receptor (P4r) in mouse sperm during maturation and capacitation and to make the first steps toward a character...
Specific Order in the Appearance of Protein Tyrosine Phosphorylation Patterns Is Functionally Coordinated With Dog Sperm Hyperactivation and Capacitation
Kinetics hyperactivation membrane destabilization sperm function phosphorylation
2008/12/19
The aims of the present study were to characterize a slow capacitation system that records initial changes in the sperm membrane state, and, using a canine model, to order the specific protein tyros...
Implication of Calmodulin-Dependent Phosphodiesterase Type 1 During Bovine Sperm Capacitation
Vinpocetine rolipram cyclic AMP in vitro fertilization motility
2008/12/18
Phosphodiesterases (PDEs) are enzymes that degrade cyclic nucleotides. The calcium-calmodulin dependent PDE type 1 (PDE 1) and the cyclic adenosine monophosphate (cAMP)-specific PDE type 4 (PDE 4) h...
Lignosulfonic Acid Blocks In Vitro Fertilization of Macaque Oocytes When Sperm Are Treated Either Before or After Capacitation
Contraception fucoidin microbicide polysulfonation zona pellucida
2008/12/16
Lignin-derived macromolecules (LDMs) are biologically active compounds that affect a variety of cell-to-cell interactions including the inhibition of fertilization and embryo development in a number o...
Activation of Protein Kinase A During Human Sperm Capacitation and Acrosome Reaction
Phosphodiesterases cyclic adenosine monophosphate calcium ionophore A23187 signal transduction
2008/12/15
Spermatozoa undergo a variety of changes during their life that are prerequisites to their maturation and ability to fertilize eggs. Mammalian sperm capacitation and acrosome reaction are regulated by...
Capacitation and Induction of the Acrosome Reaction in Bull Spermatozoa With Norepinephrine
Catecholamines bovine
2008/12/3
Identification of norepinephrine (NE) within the microenvironment of the bovine oviduct suggests a potential role for catecholamines in the events surrounding fertilization. Previous studies have ...
Capacitation and Induction of the Acrosome Reaction in Bull Spermatozoa With Norepinephrine
Catecholamines bovine
2008/12/3
Identification of norepinephrine (NE) within the microenvironment of the bovine oviduct suggests a potential role for catecholamines in the events surrounding fertilization. Previous studies have ...