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为研究小型猪复合麻醉剂（XFM）对大鼠中枢神经系统p-p38蛋白表达的影响，将30只大鼠分为对照组（C组）和麻醉剂组（M组），M组又分为4个亚组：M1组（注射XFM后大鼠翻正反射消失即刻）、M2组（注射XFM后大鼠翻正反射消失后1 h）、M3组（注射XFM后大鼠翻正反射恢复即刻）和M4组（注射XFM后大鼠翻正反射恢复后1 h），各组大鼠到达预定的时间点后分别采取脑组织，应用RT-PCR法检测脑内p...
SLA-Ⅰα链和β链的原核表达与抗血清制备
链 原核表达 抗血清
2015/7/28
猪主要组织相容性复合体Ⅰ类分子又称猪白细胞抗原Ⅰ类分子（SLA-Ⅰ），参与内源性抗原在体内的递呈过程，其结构包括重链（α链）和轻链（β链）两部分。本研究首先采用RT-PCR从原代猪肺泡巨噬细胞（PAM）中扩增出SLA-Ⅰ α链胞外区基因和β链基因；继而利用原核表达系统获得包涵体形式表达的SLA-Ⅰ α链胞外区和β链重组蛋白质；将目的重组蛋白质洗涤、复性、纯化后，经Western blot及质谱技术...
为研究rfaE基因在副猪嗜血杆菌（HPS）脂寡糖（LOS）刺激猪肺泡巨噬细胞（PAMs）炎性因子（IL-1α、IL-1β、IL-6、IL-8和TNF-α）mRNA转录中的作用，提取HPS SC096株及其rfaE基因缺失株（ΔrfaE）和互补株（cΔrfaE）的LOS，分别用5和10 μg HPS-LOS、ΔrfaE-LOS、cΔrfaE-LOS和E.coli-LPS刺激PAMs，分别在2、4、6...
细粒棘球绦虫原头蚴（PSC）具有双向发育的特点，本文旨在利用SSH和生物信息学方法筛选原头蚴包囊（CW）和成虫（AW）特异性的基因。将PSC-CW和PSC-AW双相发育差减cDNA文库的差减PCR产物克隆入pGEM-T载体并测序，利用CAP3 sequence assembly在线软件、Blast2GO软件与GenBank数据库对测序结果进行分析，筛选原头蚴成囊和成虫特异性的基因，并探讨这些基因的...
旨在研究和比较牛乳源金黄色葡萄球菌黏附素分子A区（FnBPA-A）和BCD区（FnBPA-BCD）两个功能区重组蛋白质免疫后的生物学功能，并评价其作为抗原开展研制抗金黄色葡萄球菌疫苗的价值。利用原核表达载体pET-28a分别表达重组蛋白质FnBPA-A与FnBPA-BCD，比较二者的黏附特性，并用纯化后的重组蛋白质单独及联合免疫方式免疫新西兰白兔和小鼠，分析比较各组蛋白质诱导的特异性抗体抑制菌体及...
Serotonin, GnRH-A, and dopamine interaction in the control of in vivo luteinizing hormone release in Prussian carp (Carassius gibelio Bloch) at the time of gonad recrudescence
gonadotropin-releasing hormone analoque LH
2015/7/7
The effects of serotonin (5-HT), GnRH analogue (D-Ala6
LHRH, GnRH-A) and dopamine
antagonist – pimozide (PIM), on luteinizing hormone (LH) release in mature Prussian carp female (Carassius
gibel...
Association of a synonymous mutation of the PGAM2 gene and growth traits in rabbits
SNPs body weight average daily weight gain
2015/7/7
The association results revealed the CT genotype of c.195C>T was associated
significantly (P < 0.05) with greater body weight at 84 days of age (BW84) and with average daily weight gain
(ADG). How...
Effect of prolonged aflatoxin B1 administration on blood serum oestradiol-17β and progesterone concentrations of goats during the anoestrus period
aflatoxins mycotoxins ovarian steroids
2015/7/7
The effect of prolonged aflatoxin B1 (AFB1) administration on blood serum oestradiol-17β and
progesterone concentrations in goats during the anoestrus period was investigated. Thirty Greek indigenou...
Influence of different morphological parts of buckwheat (Fagopyrum esculentum) and its major secondary metabolite rutin on rumen fermentation in vitro
Fagopyrum esculentum ruminant feed
2015/6/23
It was hypothesized that buckwheat, especially its flowers, influences foregut fermentation in
ruminant animals because it is rich in phenolic compounds. The entire fresh aerial buckwheat herb, or i...
Strategies for haplotype-based association mapping in a complex pedigreed population
complex pedigree false positives
2015/6/23
In association mapping, haplotype-based methods are generally regarded to provide higher
power and increased precision than methods based on single markers. For haplotype-based association
mapping...
Estimation of genetic parameters for melanoma in the Old Kladruber horse
melanoma linear model threshold model
2015/6/23
The aim of this study was to assess the prevalence of melanoma to investigate a possible genetic
variation of this trait in the Old Kladruber horse. A total of 564 grey varieties of the Old Kladrube...
Effect of dose of thymol and supplemental flavours or camphor on palatability in a choice feeding study with piglets
thymol camphor flavour aroma
2015/6/23
Thymol’s antimicrobial properties urged researchers to study its effect on animal performance
and intestinal health in pigs. However, thymol has the characteristic sharp odor of thyme and a bitter, ...
Lactation curves for milk yield, fat, and protein content in Slovenian dairy sheep
dairy sheep milk traits test-day records
2015/6/23
Lactation curves for daily milk yield, fat, and protein content in three dairy sheep breeds were
estimated by the repeatability animal model using test-day records. A total of 38 983 records from 30...
Influence of Lippia citriodora verbascoside on growth performance, antioxidant status, and serum immunoglobulins content in piglets
verbascoside immunoglobulin
2015/6/23
Phytogenic feed additives are used in animal feeding to improve livestock performance. The
aim of this study was to determine whether a dietary plant extract from Lippia citriodora, standardized for...
Relationship between milk yield, stage of lactation, and some blood serum metabolic parameters of dairy cows
cow milk aminotransferase cholesterol
2015/6/23
The aim of the study was to evaluate the effect of milk yield and stage of lactation on the
activity of liver enzymes, cholesterol, and vitamin C concentration in blood of milking cows.