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Luminescence-Based MicroRNA Detection Methods
microRNA Bioluminescence Nucleic Acid Detection Fluorescence
2015/5/21
MicroRNAs (miRNA) are short, 18-24 nucleotide long noncoding RNAs. These small RNAs, which are initially transcribed in the nucleus, are transported into the cell cytoplasm where they regulate protein...
Diagnostic Efficacy of Vessel Specific Coronary Calcium Score in Detection of Coronary Artery Stenosis
Computed Tomography Coronary Angiography Coronary Artery Disease
2015/10/16
Background: Coronary artery calcification which is determined quantitatively by coronary calcium scoring has been known as a sign of coronary stenosis and thus future cardiac events; hence it has been...
Detection of Class I Integrons in Staphylococcus aureus Isolated From Clinical Samples
Staphylococcus aureus Drug Resistance Polymerase Chain Reaction
2015/10/15
Background: Staphylococcus aureus is a major pathogen, causing variety of diseases and death in Iran and in the world. Despite the use of a spectrum of new antibiotics, this organism has caused severe...
Detection of Sugar Chain Expression in Hydatidiform Mole Using Lectin Histochemistry
Hydatidiform Mole Lectins Histochemistry Carbohydrates
2015/10/12
Background: Hydatidiform moles carry a significant risk for developing persistent gestational trophoblastic disease. Lectins are useful tools to identify cellular glycosylation pattern and changes in ...
Serologic Detection of Anti Toxoplasma gondii Infection in Diabetic Patients
Diabetes Infection Serum Toxoplasma gondii
2015/10/8
Background: Toxoplasmosis is caused by the Toxoplasma gondii parasite. The parasite is intracellular and can result in severe complications leading to death in immuno-deficient patients in particular....
Detection of Sugar Chain Expression in Hydatidiform Mole Using Lectin Histochemistry
Hydatidiform Mole Lectins Histochemistry Carbohydrates
2015/9/30
Background: Hydatidiform moles carry a significant risk for developing persistent gestational trophoblastic disease. Lectins are useful tools to identify cellular glycosylation pattern and changes in ...
Conventional Agar-Based Culture Method, and Nucleic Acid Amplification Test (NAAT) of the cppB Gene for Detection of Neisseria gonorrhea in Pregnant Women Endocervical Swab Specimens
Pregnant Women Neisseria Gonorrhea Culture Nucleic Acid Amplification Techniques (NAAT)
2015/9/29
Background: Neisseria gonorrhea is the etiological agent of the sexually transmitted disease (STD) gonorrhea, and primarily infects the mucous membranes of the urethra, endocervix, pharynx or rectum o...
Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
E.coli O157:H7 Stx-1 gene Detection FLASH-PCR
2015/9/28
Background: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome i...
Detection of Drug Resistance Gene in Cutaneous Leishmaniasis by PCR in Some Endemic Areas of Iran
Cutaneous Leishmaniasis Drug resistance PCR Iran
2015/9/24
Background: Cutaneous leishmaniasis is still a health problem in many rural and urban regions of Iran and drug resistance has emerged as a major impediment in the treatment of leishmaniasis. This stud...
Expression and Purification of Recombinant Mycobacterium Tuberculosis (TB) Antigens, ESAT-6, CFP-10 and ESAT- 6/CFP-10 and Their Diagnosis Potential for Detection of TB Patients
Mycobacterium tuberculosis ELISPOT CFP-10 ESAT-6 Tuberculosis Diagnosis
2015/9/23
Background: One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M. tuberculosis) genome sequence ...
The Use of pp150 and gp116 Synthetic Peptides in the Detection of CMV Antibodies
CMV IgM IgG Fusion protein Peptides pp150 gp116 gp H
2015/9/22
Background: Human cytomegalovirus (HCMV) infection may cause severe outcomes in transplant recipients and pregnant women. Diagnosis of CMV infections by using serological detection of CMV specific ant...
Detection and Prevalence of Polyoma Virus BK among Iranian Kidney Transplant Patients by a Novel Nested-PCR
BK virus Renal transplant recipients Prevalence Iran
2015/9/21
Background: BK virus is an increasingly recognized pathogen in transplant recipient patients associated with nephropathy and emerged as a cause of allograft failure linked to immunosuppressive regimen...
Application of hight performance liquid chromatography with spectrofluorimetric detection for determination of human milk retinol
Determination All-trans retinol Specrofluoromemetric detection
2009/12/31
World health organization has introduced concentration of vitamin A in breast milk as a proper indicator for characterization of vitamin A deficiency in lactating mothers and their infants (<6 mo). A ...
COMPARISON OF IMMUNOFLUORESCENCE AND,ELISA IN THE DETECTION OF MALARIAL ANTIBODIES IN SOUTHERN IRAN
IFAT IMMUNOFLUORESCENCE MALARIAL ANTIBODIES
2009/12/30
In order to compare the enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody technique (IFAT) in detection of malarial antibodies, 652 sera samples collected in the Health Surve...
Comparison of biodistribution of ¹¹¹In-Tropolone leukocytes and 125I-human nonspecific polyclonal IgG in normal and induced inflammation mice for detection of inflammation
Immunoscintigraphy Acid citrate dextrose (ACD) Hydroxy ethyl starch (HES) Immunoglobulin (Ig) Percent injected dose per gram tissue (%ID/g)
2009/12/25
Human nonspecific polyclonal IgG and granulocytes, which accumulate in inflammation foci, were radiolabeled with 125I and ¹¹¹In-Tropolone, respectively. Biodistribution of these two rad...