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Fluorescence microscopy methods for the determination of somatic cell count in raw cow’s milk
microscopy cow milk fluorescence flow cytometry temperature somatic cell wall temperature DMSCC
2016/12/8
The direct fluorescence microscopy method with ethidium bromide staining can be used for somatic cell counting in raw cow’s milk. However, this method has some limitations that may influence the resul...
Effect of the FGF2 SNP11646 on milk production and fertility traits of Holstein cattle
dairy cow fibroblast growth factor single nucleotide polymorphism linear mixed model
2016/9/1
The objective of this study was to estimate the effect of a single-nucleotide polymorphism (SNP11646) in the FGF2 (Fibroblast Growth Factor 2) gene on the deregressed proof of breeding values (DRP) of...
Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions
phagocytic chemotactic activity cortisol IL-8 CXCR1 selectin integrin
2016/6/3
The goal of this work was to understand how polymorphonuclear leukocytes (PMN), the first line of cellular defence, integrate and prioritize multiple chemotactic signals to navigate during periods of ...
Effect of the ratios of acetate and β-hydroxybutyrate on the expression of milk fat-and protein-related genes in bovine mammary epithelial cells
ratio of short chain fatty acids milk fat precursor dairy cow milk fat milk protein gene expression
2015/12/17
The objective of this study was to evaluate the effects of the different ratios of acetate and β-hydroxybutyrate (BHBA) on cell viability, triacylglycerol (TAG) content, and mRNA expression of the gen...
Genetic relationship of functional longevity with female fertility and milk production traits in Czech Holsteins
Holstein cattle genetic parameters milk yield functional traits
2015/6/24
The objectives of this study were to estimate heritabilities of and genetic correlations among longevity, milk production, and female fertility traits of Holstein cows. Fertility traits were days open...
Association of insulin-like growth factor I gene polymorphisms (IGF1/TasI and IGF1/SnaBI) with the growth and subsequent milk yield of Polish Holstein-Friesian heifers
cattle body weight insulin-like growth factors milk performance
2015/6/24
The aim of this study was to estimate potential relationships between insulin-like growth factor I gene polymorphisms (IGF1/TasI and IGF1/SnaBI) and the growth parameters of calves as well as their su...
Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits
milk yield fat percentage dairy goat SNP PCR-RFLP SSCP additive effect
2015/6/24
Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the...
Growth of bifidobacteria in mammalian milk
human milk colostrum of swine cow’s milk sheep’s milk rabbit’s milk lysozyme
2015/6/24
Microbial colonization of the mammalian intestine begins at birth, when from a sterile state a newborn infant is exposed to an external environment rich in various bacterial species. An important grou...
Genetic and environmental parameters estimation for milk traits in Slovenian dairy sheep using random regression model
dairy ewes test-day records orthogonal Legendre polynomials heritability additive genetic correlations
2015/6/24
(Co)variance components for daily milk yield, fat, and protein content in Slovenian dairy sheep were estimated with random regression model. Test-day records were collected by the ICAR A4 method. Anal...
A new internal standard for HPLC assay of conjugated linoleic acid in animal tissues and milk
sorbic acid internal standard CLA isomers HPLC photodiode array detection biological samples
2015/6/9
A new method for the quantification of underivatized conjugated linoleic acid (CLA) isomers and CLA-metabolites by silver ion liquid chromatography (Ag+-HPLC) with photodiode array detection (DAD) is ...
Analysis of raw cow milk quality according to free fatty acid contents in the Czech Republic
cow raw milk bulk milk sample mammary gland fat free fatty acids crude protein casein lactose somatic cell count total mesophilic bacteria count psychrotrophic bacteria count urea mechanical stress lipolysis
2015/6/5
The concentration (c) of free fatty acids (FFAs) in milk is an indicator of dairy cow nutrition, milk straining, its bacterial contamination and storage quality. High FFA concentrations (cs) caused by...
Factors affecting milk yield and ewe’s lactation curves estimated with test-day models
Tsigai Improved Valachian Lacaune milk yield environmental effects lactation curve
2015/6/2
Test-day records of purebred Tsigai, Improved Valachian and Lacaune ewes were analysed with a general linear model in order to investigate the effects of flock-test day, lactation number, days in milk...
Change of amino acid profile in Charolais cows’ colostrum and transient milk during the first week post partum
Charolais cows colostrum transient milk amino acids
2015/6/2
In this study the change in amino acid profile in cow’s colostrum and transient milk during the first week after parturition was examined in a Hungarian Charolais herd. Experiments were carried out wi...
Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh
milk protein genes cattle caseins beta-lactoglobulin milk production
2015/6/2
Genotypes of milk protein genes, alphaS1-casein (CSN1S1), beta-casein (CSN2), kappa-casein (CSN3) and beta-lactoglobulin (LGB), were detected in 440 individuals of Czech Fleckvieh breed using a PCR-RF...
Prevalence of mastitis pathogens in milk from clinically healthy cows
mastitis bovine intramammary IMI etiology epidemiology macrorestriction
2015/4/16
A total of 669 individual cow milk samples originating from asymptomatic cows from 16 dairy farms were examined for the presence of microorganisms with the potential to cause mastitis. Coagulase-negat...