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Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis
Pyrosequencing Microbiota Gastrointestinal Tract Sections, Sheep
2017/3/7
The gastrointestinal tract of sheep contain complex microbial communities that influence numerous aspects of the sheep’s health and development. The objective of this study was to analyze the composit...
Genetic characterization of introduced Tunisian and French populations of pike-perch (Sander lucioperca) by species-specific microsatellites and mitochondrial haplotypes
zander nuclear markers cytochrome b stocking North Africa Europe
2016/5/11
The pike-perch is among the economically most valuable fish species for both commercial and recreational fishermen. This could be seen as the main reason for its introductions into Western Europe (inc...
Chemical characterization and in vitro biological activity of four tropical legumes, Styzolobium aterrimum L., Styzolobium deeringianum, Leucaena leucocephala, and Mimosa caesalpiniaefolia, as compared with a tropical grass, Cynodon spp. for the use in ruminant diets
rumen fermentation gas production kinetics PEG
2015/6/23
Kinetic parameters were estimated
by an exponential model. STA, STD, and LEU contained higher (P < 0.05) crude protein than MIC, which
had greater neutral detergent fibre and acid detergent fibre....
Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland
Campylobacter spp. retail meat antimicrobial resistance virulence factors
2015/4/16
The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes ...
Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report
calicivirus RT-PCR phylogenetic analysis genogroup enteritis
2015/4/15
Porcine sapoviruses, members of the family Caliciviridae, have been considered as an aetiological agent of gastroenteritis in pigs. In this study, we analysed 251 faecal samples obtained from 3 to 90 ...
Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples
Listeria seasonal variation PCR-RFLP RAPD
2015/4/15
A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from Februar...
Characterization of two BHV-4 strains isolated in the Czech Republic
Bovine herpesvirus 4 (BHV-4) Restriction endonuclease analysis Southern blot
2015/4/8
This study describes the isolation of bovine herpesvirus 4 (BHV-4) from the respiratory tract of animals suffering from respiratory disease. DNA of new isolates, CH and Ni, was cleaved with BamHI, Eco...
Characterization of enterococci of animal and environmental origin using phenotypic methods and comparison with PCR based methods
Enterococcus identification D-alanin-D-alanin ligase PCR
2015/4/8
The purpose of this study was to evaluate the discriminatory power of ddl-PCR (D-alanin-D-alanin ligase PCR) and ITS-PCR (Internal Transcribed Spacer PCR) for accurate identification of enterococcal s...
Characterization of drug metabolizing enzymes and assessment of aging in the gilthead seabream (Sparus aurata) liver
Sparus aurata gilthead seabream drug metabolizing enzymes
2015/4/7
Organic anthropogenic compounds such as polycyclic aromatic hydrocarbons, polychlorinated biphenyls, nitroaromatics, dioxins, various pesticides and natural compounds can enter the aquatic environment...
Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents
diarrhoea cattle calves
2015/4/2
Faecal samples from 230 diarrhoeic and healthy calves aged 0–6 weeks, from 100 farms in Austria, were examined between October 2004 and February 2005 for the presence of bacteria, especially Shiga tox...
Molecular characterization and phylogenetic analysis of a canine parvovirus isolate in India
canine parvovirus PCR MDCK
2015/4/1
Canine parvovirus 2 (CPV-2) is the causative agent of acute hemorrhagic enteritis and myocarditis in dogs. In this study the nucleotide sequence of the VP1/VP2 gene of a CPV isolate from India was ana...
purification and characterization of glucose 6-phosphate dehydrogenase from rainbow trout (oncorhynchus mykiss) erythrocytes
purification characterization glucose 6-phosphate dehydrogenase
2015/2/5
Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) from rainbow trout (Oncorhynchus mykiss) erythrocytes was purified, using a simple and rapid method, ...
Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs
phenotypic properties virulence marker genes antibiotic resistence
2015/2/5
A total of 90 Escherichia coli O157 isolates recovered from humans, cattle, and pigs, were examined for the presence of the H7 antigen, ability to produce Shiga toxins and enterohemolysin as well as f...
Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis
Streptococcus bovis paraquat superoxide dismutase glutathione peroxidase
2015/1/26
Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide und...
Immunohistochemical characterization of efferent neurons innervating the oviduct in the pig located in the sympathetic chain ganglia
sympathetic chain ganglia oviduct pig immunohistochemistry tracing
2015/1/26
The present study was aimed at disclosing the pattern(s) of putative co-incidence of tyrosine hydroxylase (TH), dopamine -hydroxylase (DH), neuropeptide Y (NPY), substance P (SP), calcitonin gene-rela...