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Cloning and characterization of the first actin gene in Chinese oak silkworm, Antheraea pernyi
Antheraea pernyi actin cloning expression pattern
2011/1/11
The first actin gene in Chinese oak silkworm, Antheraea pernyi (Apactin) was cloned and characterized. Remarkably, two types of the Apactin cDNA clones were isolated from the pupal cDNA library, which...
家蚕核型多角体病毒山东株p35基因的克隆及序列分析Cloning and Sequencing of p35 Gene from Bombyx mori Nuclear Polyhedrosis Virus Strain Shandong
家蚕核型多角体病毒山东株 p35基因 克隆 序列分析
2008/1/16
摘要根据家蚕核型多角体病毒T3株p35基因非编码区序列设计一对特异性引物,应用PCR技术从家蚕核型多角体病毒山东株中扩增得到一条1 080 bp的片段。测序结果表明,该片段含有p35基因,开放阅读框长900 bp,编码299个氨基酸,分子量为34.91kDa,pI=6.4。序列分析表明,BmNPV山东株的p35基因与T3株存在差异。
Abstract:According to the 5′ an...