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Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos
Myostatin Zinc-finger Nucleases Knockout, Sheep
2016/11/10
Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause “double-muscling” trait in animals. In order to block the inhibiting effect of M...
Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
activin A SCNT embryo development bovine
2015/6/8
To improve the culture system of bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early developmen...
Comparison of two vitrification methods for cryopreservation of porcine embryos
pig embryo transfer
2015/6/1
The aim of this study was to compare two vitrification methods of porcine perihatching blastocysts with regard to the success of transfer of these embryos to the recipients. Expanded, hatching, or hat...
Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System
Knockout Pig Reporter Based Enrichment Somatic Cell Nuclear Transfer Zinc Finger Nuclease
2016/5/16
To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to cr...
Comparative Analysis of Nkx2-5/GATA4/TBX5 Expression in Chicken, Quail and Chicken-quail Hybrids during the Early Stage of Cardiac Development in Embryos
Cardiac Development Embryogenesis Gene Expression Chicken Quail Chicken-quail Hybrids
2016/5/11
The present study makes an investigation into expression of genes related to cardiac development in chicken, quail and chicken-quail hybrids during the early stage of embryogenesis. Real-time PCR was ...
Differentiated Human Embryonic Stem Cells Enhance the In vitro and In vivo Developmental Potential of Mouse Preimplantation Embryos
Mouse Embryo d-hES Feeder Cell Co-culture Developmental Potential
2016/5/5
In differentiating human embryonic stem (d-hES) cells there are a number of types of cells which may secrete various nutrients and helpful materials for pre-implantation embryonic development. This st...
The Effect of Various Concentrations of Taurine during In vitro Fertilization on the Development of Bovine Embryos Fertilized with Spermatozoa from Three Different Bulls
Taurine In vitro Fertilization Embryonic Development Bull Variation
2016/5/5
We investigated the effect of various concentrations of taurine during in vitro fertilization (IVF) on the embryonic development up to the blastocyst stage of bovine oocytes fertilized with three diff...
γ-LA-Supplementation to IVC for IVP Bovine Embryos
γ-Linolenic Acid Freezability Post-thaw Survival In vitro Produced Bovine Embryo Trophoblastic Cells
2016/5/3
The present study aimed to examine the effects of -linolenic acid (GLA) supplementation to in vitro culture (IVC) medium on in vitro developmental competence, freezability and morphology of in...
Changes in Plasma Estrogen Concentrations during the First Trimester of Gestation in Dairy Cows:Comparison with the Origin of Embryos and Fetal Number
estradiol-17β estrone estrone sulfate in vitro fertilization multiple pregnancies
2009/9/10
The objectives of this study were to determine the profiles of plasma estrogen concentrations during the first trimester of gestation and to correlate them with the origin of the embryos and fetal num...
Developmental Stability of Broiler Embryos in Relation to Length of Egg Storage Prior to Incubation
chickens embryonic development leg bones relative asymmetry stress
2009/7/31
The effects of storage of eggs for 0, 2, 8, 14, or 18 days prior to incubation, as measured by growth and developmental homeostasis of bilateral traits, were studied in broiler embryos. Data were obta...
Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants
chicken cryoprotectants PGCs recovery-rate viability
2009/7/30
Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial...
Developmental Enhancement of Intracytoplasmic Sperm Injection (ICSI)-Generated Quail Embryos by Phospholipase Cζ cRNA
blastoderm embryo development ICSI phospholipase Cζ quail
2009/7/30
The present study was conducted to improve the rate and stage of blastoderm and embryo development of quail oocytes by intracytoplasmic sperm injection (ICSI) with quail phospholipase Cζ (PLCζ) cRNA. ...
Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs
chicken chimera primordial germ cell quail soft x-ray
2009/7/29
The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the...
Effects of Busulfan Sustained-release Emulsion on Depletion and Repopulation of Primordial Germ Cells in Early Chicken Embryos
busulfan sustained-release emulsion early chicken embryos germline chimeras primordial germ cells
2009/7/29
The current study aimed to determine the optimal time of busulfan (1,4-butanediol dimethanesulfonate) administration for the preparation of suitable recipient embryos to generate germline chimeras in ...
Regeneration of Muscular Dystrophy Chickens by Transplantation of Early Blastodermal Cells into Recipient Embryos
blastoderm chicken germline chimera muscular dystrophy regeneration
2009/7/29
A novel strategy has been developed to generate muscular dystrophy chickens by means of germline chimeras. Donor embryos were obtained from the New Hampshire chicken; NH-413 strain which have genes re...