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南京农业大学农学院《Nature Communica》在线发表万建民课题组“A toxin-antidote system contributes to interspecific reproductive isolation in rice”（图）
万建民 基因 栽培
2024/6/11
2023年11月19日，南京农业大学万建民院士团队在Nature Communications在线发表了题为“A toxin-antidote system contributes to interspecific reproductive isolation in rice”的研究论文，揭示了两个紧密连锁的基因组成一个毒药-解毒（toxin-antidote）元件，可选择性败育不包含该元件的花粉...
Optimisation of protoplast isolation protocols using in vitro leaves of Dendrobium crumenatum (pigeon orchid)
Dendrobium crumenatum cellulase pectinase protoplast sorbitol
2011/1/12
Protoplast isolation from in vitro leaves of Dendrobium crumenatum was carried out. Factors affecting protoplast isolation, sorbitol concentration, enzyme combinations and concentration and incubation...
Studies on Culture of Cells and Tissues of Crop Plants:III. Isolation of rice mesophyll protoplasts with a new cell wall lytic enzyme "funcelase"
Cell wall Cellulase "Onozuka" R-10 Funcelase Mesophyll Oryza sativa L
2009/8/6
Rice mesophyll protoplasts were isolated with ease and with high yield from expanded 2nd leaves, which were grown on agar plates under 23°C, 16.5 W/m2 for 25 days, by using a new cell wall lytic enzym...
Studies on Culture of Cells and Tissues of Crop Plants:I. Survey on enzymatic isolation and culture of rice leaf sheath protoplasts
BSA Cell activity Cellulase "Onozuka" R-10 Protoplasts MS-medium Rice leaf sheath
2009/8/6
To establish a method for isolation and culture of rice protoplasts, enzyme solution compositions, culture conditions, and the selection of cultivars and tissues as materials were examined. The result...
Isolation and Culture of Protoplast from Finger Millet(Eleusine coracana)Callus
Cell division Cell wall Finger millet Protoplast isolation and culture
2009/8/5
High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl-1 2, 4-D and 0.1 mgl-1 kinetin. The protoplasts were release...
Isolation and Characterization of an ADP-Glucose Pyrophosphorylase Gene from Gossypium hirsutum L.
ADP-Glucose Pyrophosphorylase Gene Gossypium hirsutum L.
2009/4/30
Roots and stems of cotton (Gossypium hirsutum L.) plants store photoassimilate as starch. Partitioning of fixed carbon between starch in vegetative storage tissues and seed is likely to impact cotton ...
Isolation and Characterization of Sequences Homologous to the Tobacco Clone Axi 1（auxin independent）from a Vicia sativa Nodule cDNA Library
axi 1 auxin gene isolation Vicia sativa
2009/1/14
In this research, partial nucleotide sequences of the axi 1 gene, which is related to auxin perception and transduction, isolated from Vicia sativa using cDNA library screening were investigated. Four...
Isolation and Analysis of the Promoter of OsRacD from Photoperiod Sensitive Genic Male Sterile Rice
photoperiod sensitive genic male sterility rice genomic library OsRacD gene
2009/1/7
By using OsRacD cDNA as probe to screen the genomic library of photoperiod sensitive genic male sterile rice line Nongken 58S, a positive clone containing 2 kb promoter and 396 bp coding region of OsR...
Isolation and Characterization of Genes Differentially Expressed in Fiber of Gossypium barbadense L.
Isolation Characterization Genes Differentially Expressed Fiber Gossypium barbadense L.
2008/12/15
Cotton breeders face many problems, such as negative association between lint yield and fiber strength, in their efforts to improve fiber quality through conventional breeding methods. The primary goa...
Isolation and functional characterization of two rapeseed genes orthologous to Arabidopsis thaliana Phenylalanine Ammonia-lyase 2
Arabidopsis thaliana Brassica napus L ortholog phenylalanine ammonia-lyase (PAL) yellow seed
2010/1/11
Two rapeseed (Brassica napus L.) phenylalanine ammonia-lyase 2 genes (BnPAL2-1 and BnPAL2-2) were isolated. The full-length cDNAs of BnPAL2-1 and BnPAL2-2 are 2422 bp and 2421 bp, with corresponding g...
小麦耐盐突变体盐胁迫下SIR73基因片段的分离和鉴定Isolation and Characterization of SIR73 Gene Fragment in salt resistant mutant of Wheat Involved in Salt Stress
小麦 盐胁迫 耐盐突变体 cDNA-AFLP技术 转录因子
2007/12/30
摘要采用cDNA-AFLP（cDNA-amplified fragment length polymorphism）技术，分析“一粒传”后代小麦耐盐突变体中，耐盐性不同的两种小麦品系盐胁迫前后的基因表达差异，得到大量与耐盐相关的基因片段。其中73号片段与人类转录因子基因有一定的同源性(相应部分的同源性32%)，命名为SIR73(salt inducible in resistance)。North...
Isolation of a 66 kDa Protein with Coagulation Activity from Seeds of Moringa oleifera
Moringa oleifera Isolation Protein Soil suspension Coagulation activity
2010/11/10
The seeds of Moringa oleifera contain natural coagulants which can be used for clarification
of water. Earlier reports have suggested mainly the presence of cationic peptides, ranging in molecular ma...
ISOLATION OF BORRELIA AFZELII FROM OVERWINTERING CULEX PIPIENS BIOTYPE MOLESTUS MOSQUITOES
Culex (Culex) pipiens s.l Borrelia afzelii PCR- RFLP analysis
2010/12/23
A total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus Culex, Aedes and Anopheles were collected during the period March 2000-April 2001 from the l...
Isolation of Plant-Growth-Promoting Endophytic Bacteria from Bean Nodules
bean nodule Bacillus sp plant-growth-promoting bacteria Department of Biology, Sunchon National University
2010/11/8
Plant growth promoting rhizobacteria (PGPR) was isolated from bean (Phaseolus vulgaris L.) nodules. DLA strain was found to increase bean yield and growth when plant were co-inoculated with Rhizobium ...