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Climate-smart forestry (CSF) is an emerging branch of sustainable adaptive forest management aimed at enhancing the potential of forests to adapt to and mitigate climate change. It relies on much high...
The theoretical concept of "climate-smart?forestry" aims to integrate climate change mitigation and adaptation to maintain and enhance forests' contributions to people and global agendas. We carried o...
In the United States, Climate?Smart?Forestry?(CSF) has quickly become a popular topic within the academic, political, and industry realms, without substantial delineation of what exactly CSF is. In th...
Forests mitigate climate change by storing carbon and reducing emissions via substitution effects of wood products. Additionally, they provide many other important ecosystem services (ESs), but are vu...
To reduce the ecological footprint, conserve scarce resources, promote a more food secure world and to enhance and promote the value of public open space and recreational horticulture.
为了分离和克隆辣椒中疫霉菌诱导基因,以接种辣椒疫霉菌的叶片为材料,利用SMART 技术构建辣椒疫霉菌与辣椒互作的双杂交cDNA 文库。结果表明:该文库容量为3.6×106 cfu,重组率88%左右,插入片段集中在300~2 000 bp 之间,平均长度约为800 bp,表明获得的文库质量较高,该文库将为分子育种提供重要的基因资源。
用Trizol 试剂提取海带配子体总RNA , 用SMART cDNA 文库构建试剂盒构建cDNA 文库。经测定原始文库滴度达到1 .1 ×108 Pfu/ ml , 文库有500 μl 体积, 库容量为5 .5 ×107 Pfu, 通过PCR 检测, 文库的重组率达100% , 扩增出的片段主要集中在0 .8 ~1 .8 kb , 平均插入片段长度约为1 .34 kb 。这些数据表明, 已获得高...

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