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Expressions of Antimicrobial Peptide Gallinacin-1,-2 and-3 mRNAs in the Oviduct of Laying Hens
antimicrobial peptide chicken gallinacin oviduct mRNA expressions
2009/7/31
The aim of this study was to determine the profiles of mRNA expressions of gallinacin (Gal)-1, -2 and -3, which are the antimicrobial peptides, in the oviduct of laying hens. The oviductal tissues of ...
Preparation and Application for Immunocytochemistry of Antibody to Gallinacin-3,an Antimicrobial Peptide,in Chicken
antibody preparation antimicrobial peptide chicken gallinacin
2009/7/30
The aim of this study was to develop an antibody to gallinacin-3 which is one of the antimicrobial peptides in chickens. The antibody was raised in rabbit using a synthetic peptide of gallinacin-3 (Ga...
重组鸡抗菌肽Gallinacin-9的原核表达及其抗菌活性的鉴定
Gallinacins-9 鸡 融合蛋白 抗菌活性
2008/12/1
采用RT-PCR方法，从鸡舌组织中扩增到Gallinacin-9（Gal-9）基因，测序表明Gal-9为201 bp，其成熟肽由67个氨基酸残基组成。进一步将克隆的Gal-9基因亚克隆到大肠杆菌原核表达载体pGEX-6p-1的EcoRⅠ和SalⅠ双酶切位点上，构建重组表达质粒pGEX-Gal-9，经序列分析鉴定确证目的基因克隆入载体的预期位点。将重组质粒转化大肠杆菌BL21，于37 ℃诱导培养不同...