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为研究不同转基因作物T-DNA插入位点侧翼序列分离中所用的通用简并引物对TAIL-PCR的影响,本研究使用5个通用简并引物分别对8个转基因大豆、30个转基因水稻和2个转基因油菜株系进行了TAIL-PCR分析,结果表明,5个简并引物在TAIL-PCR中的扩增效果存在显著差异,同一简并引物在不同转基因物种的扩增效果显著不同。AD1引物在转基因水稻和转基因油菜中扩增效果较好,AD2引物在转基因水稻、油菜...
ABF/AREB/ABI5/DPBF类转录因子属于碱性亮氨酸类蛋白，在依赖ABA的逆境信号传导途径中起重要的作用。本研究结合该类转录因子的结构特点，利用3′ RACE技术和优化的TAIL-PCR技术成功获得棉花中三个该家族成员编码基因。进一步验证结果表明两步PCR成功获得基因完整的ORF、3′ UTR以及部分5′ UTR序列。本研究所获基因为棉花抗逆基因工程提供了候选基因源，优化后的TAIL-PC...
Absorption of Spring Applied Nitrogen in Satsuma Mandarin Orchard Covered with Rat’s-tail Fescue,Vulpia myuros
15N-tracer method Satsuma mandarin Sod culture
2009/7/9
A 15N-tracer method was used to study the absorption of nitrogen applied in the spring to Satsuma mandarin trees and Rat’s-tail fescue, Vulpia myuros, in a mandarin orange orchard covered with Rat’s-t...
适于TAIL-PCR 模板的水稻基因组DNA 提取方法的优化
水稻 基因组DNA 提取方法
2009/7/6
[ 目的] 筛选适合作为TAIL- PCR 模板的水稻基因组DNA 提取方法。[ 方法] 采用5 种方法提取水稻叶片基因组DNA, 筛选出DNA
纯度及浓度较高的、适合作为TAIL-PCR 模板的水稻基因组DNA 提取方法。[ 结果] 改良的SDS 法及CTAB 法提取的基因组DNA 纯度及
浓度较高, 效果明显优于脲法及简易法。以TAIL- PCR 技术对选择的同一个含Ds 元件的样品的5...
Hydroxyanisole on Some Properties of Kidney Fat and Tail Fat During Frozen Storage
Hydroxyanisole Properties of Kidney Fat Tail Fat Frozen Storage
2009/1/23
Some physical and chemical properties of kidney fat (obtained from cattle) and tail fat (obtained from sheep) were determined in this study. Effects of butylated hydroxytoluene (BHT), butylated hydrox...
Absorption of Nitrogen Decomposed from Rat Tail Fescue Residue in Satsuma Mandarin Orchard with Sod Management
Nitrogen absorption 15N-labeled rat tail fescue Satsuma mandarin
2010/12/2
The nitrogen dynamics in a sod culture of the satsuma mandarin orchard were determined by using 15N-labeled rat tail fescue (Vulpia myuros). 15N-labeled rat tail fescue plants were applied to the soil...
Absorption of Fall-Applied Nitrogen in Satsuma Mandarin Orchard with Rat Tail Fescue as Cover Crop
Cover crop Fall-applied nitrogen 15N-tracer method
2010/12/2
A 15N-tracer method was used to study the absorption of nitrogen applied in the fall to satsuma mandarin trees and Rat tail fescue, Vulpia myuros, in a pot test in which a satsuma mandarin tree was gr...
Association of a Single Codon Deletion in Bone Morphogenetic Protein 15 Gene with Prolificacy in Small Tail Han Sheep
Sheep Prolificacy Bone Morphogenetic Protein 15 Gene PCR-SSCP
2016/4/18
Small Tail Han Sheep has significant characteristics of high prolificacy and non-seasonal ovulatory activity and is an excellent local sheep breed in P. R. China. Recently a novel member of the transf...
Association between PCR-RFLP of Melatonin Receptor 1a Gene and High Prolificacy in Small Tail Han Sheep
Sheep Prolificacy Reproductive Seasonality Melatonin Receptor 1a Gene PCR-RFLP
2016/4/14
Melatonin regulates circadian rhythms and reproduction changes in seasonally reproductive mammals through binding to high-affinity, G-protein-coupled receptors. Small Tail Han sheep that has significa...
Association Analysis between Five Microsatellite Loci and Litter Size in Small Tail Han Sheep
Sheep Litter Size Microsatellite Polymorphism Least Squares Analysis
2016/4/14
The objective of the present study was to explore associations between five microsatellites linked to FecB and FecXI genes and litter size in Small Tail Han sheep. The polymorphisms of five microsatel...