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Proteomic Analysis of Bovine Pregnancy-specific Serum Proteins by 2D Fluorescence Difference Gel Electrophoresis
2D DIGE CyDye Proteomics Bovine Pregnancy Pregnancy-specific Serum Proteins
2016/5/18
Two dimensional-fluorescence difference gel electrophoresis (2D DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein ex...
O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in Korea
Escherichia coli piglet O-serogroup pathotype virulence gene
2015/4/16
A total of 116 Escherichia (E.) coli isolates isolated from neonatal diarrhoeic piglets were serogrouped and tested for the presence of virulence genes for fimbrial and non-fimbrial adhesins, intimin,...
Intermediately virulent Rhodococcus equi isolates from pigs in Slovenia: discovery of new plasmid types and assessment of genetic diversity by pulsed-field gel electrophoresis
genotyping molecular epidemiology restriction enzyme analysis
2015/4/2
The presence of large plasmids in 30 Rhodococcus equi strains isolated from pig lymph nodes with granulomatous changes was investigated. Plasmid DNAs were isolated and digested with the restriction en...
Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods
E. coli O157 cattle pigs virulence marker genes RFLP genetic relatedness
2015/1/26
Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC)...
Characterisation of Oat Genetic Resources Using Electrophoresis of Avenins
avenins genotype distinguishing line oats PAGE
2014/2/25
The prolamin (avenin) patterns of oat (Avena sativa L.) cultivars released in the Czech Republic and in the former Czechoslovak Republic were analysed by acid polyacrylamide gel electrophoresis (A-...
Seed protein electrophoresis for assessment of genetic variation within genotypes of meadow fescue (Festuca pratensis Huds)
acid-PAGE genetic diversity meadow fescue SDS-PAGE
2014/3/10
The protein diversity of 45 genotypes (forty genotypes selected within two gene pools and five varieties) of meadow fescue was examined. Genetic variation was described using ISTA/UPOV methods for cro...
Simultaneous Analysis of Nitrates and Major Organic Acids in Spinach Using Capillary Electrophoresis
Organic Acids Nitrates Capillary Electrophoresis
2009/7/10
It is necessary to decrease the concentrations of nitrate and oxalate in spinach for human health. This analytical method was developed to simultaneously determine nitrate, oxalate and other major org...
Application of multiple enzyme restriction fragment length polymorphism analysis and microchip electrophoresis for estimation of antibiotic-tolerant bacterial group
polymyxin B resistant bacteria field soil livestock feces microchip electrophoresis
2009/3/5
A combined method of microchip electrophoresis and multiple enzyme restriction fragment length polymorphism analysis, by which bacterial phylogeny was estimated by comparing the measured multiple en...
Correlation Analysis between the Breeding Value of Carcass Traits in Hanwoo (Korean Brown Cattle), Bos Taurus, L. and Spot Intensity on Two-dimensional Gel Electrophoresis
Hanwoo Proteomics Spot Intensity Two-Dimensional Gel Electrophoresis Breeding Value
2016/4/22
In order to investigate the genetic marker associated with economic performance in Hanwoo (Korean Brown Cattle), proteomic approach was used. Breeding values were estimated from performance tested ste...
Characterisation of Potato (Solanum tuberosum L.) Varieties by Electrophoresis of Tuber Proteins
potato variety characterisation tuber proteins electrophoresis
2014/3/13
The modified PAGE method (TRIS – Glycine buffer pH 8.9) was used for the characterisation of
selected 25 registered potato varieties. This method enabled to identify each variety from the examined v...
Two-dimensional Electrophoresis of Soluble Proteins and Profile of Some Isozymes Isolated from Maize Plant in Response to Nacl
isozymes maize polypeptide level two-dimensional polyacrylamide gel electrophoresis
2010/11/8
Maize genotype (Zea mays L) single cross 124 was grown in water culture in presence or absence of 150 mM NaCl for 15 days. Inhibition of plant growth and modification of plant morphology are the most ...
GENETIC DIFFERENTIATION OF COTTON CULTIVARS BY POLYACRYLAMIDE GEL ELECTROPHORESIS
Cluster analysis cotton genetic diversity Gossypium hirsutum & polyacrylamide gel electrophoresis
2010/11/30
Information about genetic diversity and relationships among the breeding materials has a significant impact on crop improvement. Association between parental divergence and progeny performance has not...
Assessment of Frost Tolerance of Wheat Doubled Haploids by Gliadin Electrophoresis
gliadins frost tolerance marker doubled haploids wheat
2014/3/19
The relationship between gliadin alleles, known to mark frost tolerance, and actual frost tolerance was investigated in 52 doubled haploids (DH) from the cross Florida× Vlada. Frost tolerance was expr...