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Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism
BAX Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Screening
2017/3/9
This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated...
Rapid sexing of selected Galliformes by polymerase chain reaction
fowl game fowl polymerase chain reaction
2015/6/23
Vent sexing of one-day-old chicks in commercial hatcheries has long been common practice
and can be highly accurate. However, there are circumstances when this technique is not applicable such
as ...
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
2015/5/25
Therefore, culture-independent detection methods such as the polymerase chain reaction
are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay
...
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
2015/4/30
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell cultu...
Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction
budgerigar fledgling disease Melopsittacus undulatus parrots viruses PCR
2015/3/13
High mortality of nestling cockatiels (Nymphicus hollandicus) was observed in one breeding flock in Slovakia. The nestling mortality affected 50% of all breeding pairs. In general, all the nestlings i...
Polymerase chain reaction assay for the diagnosis of experimentally infected pregnant Sprague-Dawley rats with Brucella abortus biotype 1
Sprague-Dawley rats polymerase chain reaction South Korea
2015/2/5
In order to diagnose the experimentally infected pregnant Sprague-Dawley (SD) rats with Brucella abortus biotype 1 using polymerase chain reaction (PCR) assay, the SD rats were injected subcutaneously...
Identification of Xiphinema vuittenezi by polymerase chain reaction
nematodes Xiphinema vuittenezi PCR ITS primer
2015/1/30
So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies. Therefore, a polymerase chain reaction (PCR) protocol ...
Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis
Pork Contamination Detection Meatballs Polymerase Chain Reaction
2016/5/17
This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets...
Detection the 4/91 strain of infectious bronchitis virus in testicular tissue from experimentally infected rooster by reverse transcription-polymerase chain reaction
Infectious bronchitis virus testicular tissue rooster
2010/9/13
Avian infectious bronchitis (IB) was initially considered to be a disease primarily of the respiratory and female reproductive tract of chicken. Avian infectious bronchitis virus has wide and variable...
New Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
chimeric chicken germline mitochondrial DNA D-loop region single nucleotide polymorphism sperm
2009/7/31
Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such...
Highly reliable polymerase chain reaction assay for citrus huanglongbing（citrus greening disease）using the sucrose synthase gene as an internal control
citrus huanglongbing PCR assay internal control sucrose synthase gene
2009/3/16
The polymerase chain reaction (PCR) was used with an internal control to exclude false-negative reactions in a citrus huanglongbing (HLB) assay. Degenerated primers, CSSF-1 (5'-GACACTGTTGGWCAGTATGA-...
Detection of Enhanced Green Fluorescent Protein DNA in Pink Bollworm through Polymerase Chain Reaction
Green Fluorescent Protein DNA Pink Bollworm Polymerase Chain Reaction
2008/12/15
Indelible marking of pink bollworm (Pectinophora gossypiella) would assist in implementation of sterile insect technique-based pest control of this insect. Additionally, genetic manipulation through D...
PREVALENCE OF ANAPLASMA PHAGOCYTOPHILUM IN IXODES RICINUS TICKS DETERMINED BY POLYMERASE CHAIN REACTION WITH TWO PAIRS OF PRIMERS DETECTING 16S rRNA AND ankA GENES
ticks Ixodes ricinus Anaplasma phagocytophilum
2010/12/23
A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females)were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Pola...
Rapid Detection and Quantification of Rhynchosporium secalis in Barley Using a Polymerase Chain Reaction
barley scald real-time PCR pathogen diagnostics
2014/3/13
PCR primers for diagnosis of Rhynchosporium secalis in seed samples of barley were developed.
For the quantification of the pathogen in seed samples a real-time PCR with SYBR Green approach was use...
Sexing of Sheep Embryos Produced In vitro by Polymerase Chain Reaction and Sex-specific Polymorphism
Sheep Embryo Sexing PCR Polymorphism Sex Ratio
2016/4/13
The accuracy of Polymerase chain reaction (PCR) assay in sexing of sheep embryos was assessed in this study. A total of 174 ovine embryos produced in vitro at different stages of development (2, 4-8 c...