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本研究旨在检测重组结核分枝杆菌10 ku培养滤液蛋白（culture filtrate protein 10，CFP10）对肺泡Ⅱ型上皮细胞系A549细胞Toll样受体（TLRs）信号途径及其介导的炎症反应的影响。PCR扩增cfp10目的基因片段，构建重组质粒载体pCzn1-CFP10。将重组质粒pCzn1-CFP10转入BL21（DE3）大肠杆菌感受态细胞，IPTG诱导表达重组蛋白CFP10（r...
目的：研究瑞巴匹特对肺上皮细胞株A549 细胞TLR4表达及分泌肿瘤坏死因子（TNF-α）的影响。方法：用脂多糖(LPS)建立体外A549 细胞体外炎症损伤模型，实验分为对照组（无干预无刺激）、模型组（LPS刺激）、干预组1（LPS和10 mg/L瑞巴匹特）、干预组2（LPS和30 mg/L瑞巴匹特）、用ELISA观察A549细胞分泌TNF-α和RT-PCR及蛋白免疫印迹观察A549 细胞TLR4...
INFLAMMATORY POTENTIAL OF ORGANIC DUST COMPONENTS AND CHEMICALS MEASURED BY IL-8 SECRETION FROM HUMAN EPITHELIAL CELL LINE A549 IN VITRO
lung inflammation epithelium IL-8 organic dust endotoxin glucans
2010/12/27
Due to the complex composition of organic dust it is difficult to point at one or a few components causing pulmonary health problems in different occupational environments.