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脱硫菌Rhodococcus sp.LY822专一性脱硫活性及相关基因的研究
二苯并噻吩 生物脱硫 脱硫基因
2009/11/9
以专一性脱硫菌Rhodococcus sp. LY822质粒DNA为模板，利用已知的脱硫基因序列设计引物，PCR扩增得到了3个脱硫基因片段dszA, dszB和dszC. 构建了相应的表达质粒pETA, pETB和pETC，在转化大肠杆菌BL21(DE3)中表达，得到了dszA, dszB和dszC基因的融合表达产物. SDS-PAGE分析结果显示，蛋白带分子量分别为50, 40和45 kDa. ...
Inducing expression and the reaction characteristic of nitrile hydratase (NHase) from Rhodococcus sp. SHZ-1 were investigated. The results showed that the expression of NHase was greatly enhanced with...