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The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of ...
Polyphenol oxidase (PPO) and peroxidase (POD) were extracted from a new loquat fruit cultivar (Ninghaibai) and characterised using reliable spectrophotometric methods. In both cases, the optimum pH fo...
Polyphenol oxidase (PPO) from Melissa officinalis L. subsp. officinalis (lemon balm) was partially purified by ammonium sulphate precipitation and dialysis; and then it was characterised in detail in ...
An analyzing study to evaluate the relationship between Polyphenol Oxidase (PPO) activity, internal qualities and degree of browning was conducted on lettuce var. Grand Rapids in order to understand t...
In the present study glucose oxidase (GOD) has been isolated from a culture filtrate of Penicillium notatum. The enzyme was purified by ammonium sulphate precipitation, diethylaminoethyl (DEAE) cellul...
Variation of total polyphenol (TPP) and polyphenol oxidase activity (PPO) was investigated in ‘Irwin’ mango fruit in various color stages of development: green, purple, purplish red and red. Fruits us...
The effects of polyphenol oxidase (PPO) activity in wheat flour on the formation of protein-bound 5-S-cysteinyl-3, 4-di-hydroxyphenylalanine (5-S-cysteinyldopa) in gluten have been studied. The wheat ...
Bananas were coated with 2% sucrose palmitic acid ester after treatment with 100 ppm ethylene for 12 h and stored at 20°C. The use of 2% sucrose palmitic acid ester coating significantly reduced weigh...
The degree of methylesterification of pectin was measured using a modified alcohol oxidase method to determine the methanol liberated from pectin by saponification. When the alcohol oxidase method was...
Polyphenol oxidase (PPO) is transported to plastid after translation. However, we often detect PPO activity in a soluble fraction of apple. Here we examined the location of PPO in retail apples. PPO a...
Polyphenol oxidase was purified to homogeneity from Japanese wheat (Triticum aestivum cv. Tohoku 198) bran, using 4-methylcatechol as a substrate. The enzyme was purified 1670-fold with a recovery of ...
Occurrence of polyphenol oxidase (PPO, o-diphenol: oxygen oxidoreductase, EC.1.10.3.1) in the fruits of nine cultivars of banana (Musa spp.) commercially cultivated in China was investigated. All bana...
Soluble polyphenol oxidase of edible yam (Dioscorea opposita Thunb.) was purified approximately 203-fold with a recovery rate of 15% by ammonium sulfate fractionation, ion exchange chromatography, hyd...
Membrane microfiltration fermentation (MMF) with cell recycling was successfully applied to the production of glucose oxidase (GOD). A plate microfiltration module was found suitable for such purpos...
The paper presents analytical parameters of an enzymatic method for alcohol assay in alcoholic beverages by the use of a new kit based on alcohol oxidase and peroxidase catalyzed reactions (AOP). Sens...

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