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经过杀菌的发酵肠，仍有部分会变质，为探究其原因并进行控制而进行了以下试验。从商业发酵肠中分离获得1株耐热芽孢菌株B1，为构建控制技术，提高发酵肠安全水平，对该芽孢菌进行鉴定，并研究抗菌肽surfactin对其芽孢生长曲线的影响及盐类对surfactin灭活芽孢效果的影响。采用16S rDNA序列分析对该菌进行鉴定，并研究了不同浓度的NaCl和Na2HPO4?12H2O对surfactin灭活芽孢效...
基于PCR-DGGE和拟杆菌(Bacteroides)16S rRNA的岩溶地下水粪便污染来源示踪研究:以重庆南山老龙洞地下河系统为例
岩溶地下水 粪便污染 大肠菌群 拟杆菌 PCR-DGGE
2016/12/7
岩溶地下水的微生物污染日益严重,其来源的研究得到国际学术界的广泛关注.本研究以重庆南山老龙洞岩溶地下河系统为对象,采用滤膜法监测地下水中的总细菌、大肠杆菌、粪大肠菌及粪链球菌等微生物指标,以拟杆菌(Bacteriodes)为指示细菌,采用PCR-DGGE示踪地下水中大肠杆菌／粪大肠杆菌的来源.结果表明,老龙洞地下河流域各类细菌含量严重超标,总细菌数为10~2.9×107CFU·mL-1,大肠菌群总...
Novel Bacteriocinogenic Lactobacillus plantarum Strains and Their Differentiation by Sequence Analysis of 16S rDNA, 16S-23S and 23S-5S Intergenic Spacer Regions and Randomly Amplified Polymorphic DNA Analysis
bacteriocin gene differentiation Lactobacillus plantarum ribosomal intergenic spacer region
2011/2/24
Six strains of bacteriocinogenic Lactobacillus plantarum (TL1, RG11, RS5, UL4, RG14
and RI11) isolated from Malaysian foods were investigated for their structural bacteriocin
genes. A new combinatio...
Characterization of the predominant spoilage bacteria in sliced vacuum-packed cooked ham based on 16S rDNA-DGGE
Predominant spoilage bacteria Sliced cooked ham PCR-DGGE
2012/8/13
16S rDNA DGGE fingerprinting and phylogenetic analysis were used to reveal the dynamics and identification of the predominant spoilage bacterial in sliced vacuum-packed cooked ham during storage at 4 ...
硅酸盐细菌B925对烟草黑胫病菌的抑制作用及其16S rDNA序列分析。
Genotypic Characterisation of Indigenous Soybean Rhizobia by PCR-RFLP of 16S rDNA,rep-PCR and RAPD Analysis
nitrogen fixation rhizobia soybean
2009/3/19
The taxonomy of nitrogen fixing bacteria that form symbiotic associations with leguminous plants has been deeply changed in recent years. The use of very sensitive and accurate molecular methods has e...