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Mapping-by-sequencing在作物农艺性状相关基因定位的应用
Mapping-by-sequencing Bulked segregant analysis 基因定位 作物
2018/12/29
通过挖掘控制重要农艺性状的基因以提高农作物产量是育种学家一直关注的问题。然而由于农作物遗传背景复杂且基因组庞大, 该过程往往步骤繁琐, 耗时长且成本较高。Mapping-by-sequencing (利用高通量测序进行基因定位, MBS)利用二代测序技术和遗传分离群体混池建库分析的方法成功解决了上述问题, 加快了农作物重要农艺性状的基因定位同时也降低了研究成本。该方法已经成功应用于重要农艺性状相关...
Genome-Wide Identifi cation of MicroRNAs inMedicago truncatula by High-Throughput Sequencing
microRNA Medicago truncatula High-throughput sequencing Bioinformatics analysis
2015/2/5
MicroRNAs (miRNAs) are small, endogenous RNAs that play important regulatory roles in development and stress response in plants by negatively regulating gene expression post-transcriptionally.Medicago...
Ethylene-responsive miRNAs in roots of Medicago truncatula identified by high-throughput sequencing at whole genome level
Ethylene microRNA Medicago truncatula High-throughput sequencing
2015/2/5
Ethylene is one ofthe classical plant hormones with a diverse function in plant growth and development. Root elongation is sensitive to ethylene such that treatments with ethylene and the ethylene pre...
Identification of aluminum-responsive microRNAs in Medicago truncatula by genome-wide high-throughput sequencing
Aluminum toxicity High-throughput sequencing Medicago MicroRNA
2015/2/5
MicroRNAs (miRNAs) play important roles in response of plants to biotic and abiotic stresses. Aluminum (Al) toxicity is a major factor limiting plant growth in acidic soils. However, there has been li...
Identification of drought-responsive microRNAs in Medicago truncatula by genome-wide highthroughput sequencing
drought-responsive microRNAs Medicago truncatula genome-wide highthroughput sequencing
2015/2/5
Backg round: MicroRNAs (miRNAs) are small, endogenous RNAs that play important regulatory roles in development and stress response in plants by negatively affecting gene expression post-transcriptiona...