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Individual Vesicle Fusion Events Mediated by Lipid-anchored DNA
Cholesterol DNA Lipid Bilayers Phosphatidylcholines Phosphatidylethanolamines Membrane Fusion Unilamellar Liposomes
2016/5/25
Membrane fusion consists of a complex rearrangement of lipids and proteins that results in the merger of two lipid bilayers. We have developed a model system that employs synthetic DNA-lipid conjugate...
Vesicle Fusion Observed by Content Transfer Across a Tethered Lipid Bilayer
Endoplasmic reticulum-associated degradation (ERAD) ubiquitin-proteasome system (UPS) gp78/RNF45 Epidermal growth factor receptor (EGFR) Exosome Duffy antigen receptor for chemokine (DARC)
2016/5/25
Synaptic transmission is achieved by exocytosis of small, synaptic vesicles containing neurotransmitters across the plasma membrane. Here, we use a DNA-tethered freestanding bilayer as a target archit...
Covalent Attachment of Lipid Vesicles to a Fluid-Supported Bilayer Allows Observation of DNA-Mediated Vesicle Interactions
Caspian and Black Sea waters reverse-osmotic desalination Na-zeolite softening of the permeate
2016/5/25
Specific membrane interactions such as lipid vesicle docking and fusion can be mediated by synthetic DNA-lipid conjugates as a model for the protein-driven processes that are ubiquitous in biological ...
DNA-tethered Membranes Formed by Giant Vesicle Rupture
Tethered lipid membrane Surface immobilized DNA Giant unilamellar vesicles
2016/5/25
We have developed a strategy for preparing tethered lipid bilayer membrane patches on solid surfaces by DNA hybridization. In this way, the tethered membrane patch is held at a controllable distance f...
Effects of Linker Sequences on Vesicle Fusion Mediated by Lipid-Anchored DNA Oligonucleotides
DNA machine synthetic biology
2016/5/25
Synthetic lipid-oligonucleotide conjugates inserted into lipid vesicles mediate fusion when one population of vesicles displays the 5'-coupled conjugate and the other the 3'-coupled conjugate, so that...
Lipid-anchored DNA Mediates Vesicle Fusion as Observed by Lipid and Content Mixing
DNA Vesicles Lipids Fuses
2016/5/25
A general method for synthesizing 5(')- and 3(')-coupled DNA-lipid conjugates has been developed and employed in DNA-mediated vesicle fusion. Vesicles presenting complementary DNA fuse, resulting in b...
Early Steps of Supported Bilayer Formation Probed by Single Vesicle Fluorescence Assays
Adsorption Diffusion Lipid Bilayers Lipid Bilayers Liposomes Membrane Fluidity Membrane Fusion Membranes,Artificial Microscopy,Fluorescence,Multiphoton Motion Phosphatidylcholines Phosphatidylserines Xanthenes
2016/5/24
We have developed a single vesicle assay to study the mechanisms of supported bilayer formation. Fluorescently labeled, unilamellar vesicles (30-100 nm diameter) were first adsorbed to a quartz surfac...
Genetic analysis of two mutants altered with respect to formation of polyhydroxyalkanoic acid and identification of putative RNA helicase, nuclease and gas vesicle genes in {\it Bacillus megaterium\/}
Genetic analysis of two mutants altered with respect to formation of polyhydroxyalkanoic acid and identification of putative RNA helicase nuclease and gas vesicle genes in Bacillus megaterium
2015/1/28
To study polyhydroxyalkanoate (PHA) accumulation and PHA regulation in Bacillus megaterium, transposon mutagenesis with Tn917-LTV1 was carried out and seventy two mutants that produce more or less PHA...
Boundary-induced orientation of dynamic filament networks and vesicle agglomerations
Boundary-induced dynamic filament networks vesicle agglomerations
2011/9/7
We find a statistical mechanism that can adjust orientations of intracellular filaments to cell geometry in absence of organizing centers. The effect is based on random and isotropic filament (de-)pol...
The Relative Centrifugation Force Permits Visualization of the Germinal Vesicle in Pig Oocytes
Oocyte Germinal Vesicle Pig Relative Centrifugation Force
2016/4/18
Pig oocytes contain high levels of lipids in the ooplasm, which reduces the visibility of the germinal vesicle (GV) under microscopic examination. Therefore, the purposes of this study were to investi...