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The C-terminal domain (CTD) of the RNA polymerase II (RNAPII) subunit Rpb1 must exist in a hypophosphorylated state prior to forming a competent transcription initiation complex. However, during trans...
RNA polymerase II (RNAPII) is regulated by multiple modifications to the C-terminal domain (CTD) of the largest subunit, Rpb1. This study has focused on the relationship between hyperphosphorylation o...
In this dissertation, I report the functions of the TCV coat protein in TCV viral and subviral RNA replication using in vivo and in vitro systems. In chapter 2, I present my results on construction of...
The forward translocation model (paused or stalled polymerase moves forward in the absence of transcription, dissociation of the complex happens when hybrid is too short to stabilize the complex) is t...
Transcription initiates when the enzyme binds to the promoter region and melts open an initial transcribing bubble that extends from position -4 to +4. The initiation phase continues until the enzyme ...
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...

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