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Efficient Gene Transfer into Early Chicken Embryos by Electroporation of Stage X Blastoderms in Vivo, Applying Electric Pulses Vertically to the Blastoderm Layer
blastoderm chick embryo electroporation embryo culture GFP gene
2009/7/31
In order to introduce exogenous DNA into early chicken embryos by transfecting the stage X (EG&K) blastoderm, in vivo electroporation was applied to it. A pair of novel electrodes was devised for appl...
Effect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation
blastoderm electrode electroporation GFP gene
2009/7/31
In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (...
Effects of Soft X-Ray Irradiation to Stage X Blastoderm on Restriction of Proliferation of Primordial Germ Cells in Early Chicken Embryos
chick embryo primordial germ cell soft X-ray stage X blastoderm
2009/7/30
It is necessary to deplete the endogenous primordial germ cells (PGCs) from recipient embryos when germline chimaeric chickens are produced by the transfer of PGCs. In this study, effects of soft X-ra...
Z-Chromosome Specific Primers for Chicken-Quail Hybrid Blastoderm
chicken PCR PKCI quail Z-chromosome
2009/7/30
A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for t...
Dynamic Analysis of the Developmental Fate of Cells in the Center of the Area Pellucida of the Blastoderm in Chicken
migration PKH26 primordial germ cells vasa
2009/7/30
The cells in the center of the area pellucida of the blastoderm have been used as donors for production of chimeric chickens, but the developmental fate of the cells has not been analyzed in detail. A...