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Integrative Expression of Glucoamylase Gene in a Brewer’s Yeast Saccharomyces pastorianus Strain
glucoamylase brewer’s yeast diacetyl fermentation
2009/2/23
The recombinant brewer’s yeast Saccharomyces pastorianus strain was constructed by introducing the ilv2::GLA fragment released from pMGI6, carrying glucoamylase gene (GLA) and using the yeast α-acetol...
Spatial and Temporal Expression of Transferrin Gene In the Rat Mammary Gland
rat mammary transferrin in situ hybridization
2008/7/31
The distribution and concentration of transferrin mRNA in the rat mammary gland was analyzed using in situ hybridization, and the results were compared with those obtained for other milk protein mRNA....
Cloning and Expression of an Oligo-1,6-glucosidase Gene from Arthrobacter globiformis I42 and Biochemical Characterization of the Recombinant Enzyme
oligo-1 6-glucosidase Arthrobacter globiformis dextran
2008/4/20
The gene encoding an oligo-1,6-glucosidase was cloned in terms of walking downstream from the glucodextranase gene of the chromosomal DNA of Arthrobacter globiformis I42. An open reading frame consist...
Cloning, Sequencing and Expression Analysis of an Endo-1,4-β-D-Xylanase Gene from Aspergillus sojae
Aspergillus sojae xylanase arabinoxylan recombinant protein
2008/4/20
A xylanase gene was cloned from the cDNA library of Aspergillus sojae. The nucleotide sequence suggested that the mature enzyme is preceded by a 22 amino acid signal sequence and the molecular mass wa...
Bacteriophage Resistance in Lactococcus lactis Engineered by Replacement of a Gene for a Bacteriophage Receptor
phage resistance bacteriophage pip gene replacement
2008/3/27
The objective of this study was to construct a food-grade, phage-resistant strain of Lactococcus lactis by replacing a specific chromosomal gene with an allele that had been mutated in vitro. Lactococ...
Cloning and Characterization of the Galactokinase Gene from Streptococcus thermophilus
Streptococcus thermophilus galactose galactokinase
2008/3/22
The objective of this research was to clone and characterize the galactokinase gene (galK) from Streptococcus thermophilus F410. Partially digested genomic DNA was cloned into pBR322 and transformed i...