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Analysis of ENPP2 in the Uterine Endometrium of Pigs Carrying Somatic Cell Nuclear Transfer Cloned Embryos
Pig Pregnancy ENPP2 LPA SCNT
2016/5/12
Somatic cell nuclear transfer (SCNT) is a useful tool for animal cloning, but the efficiency of producing viable offspring by SCNT is very low. To improve this efficiency in the production of cloned p...
Effects of Trichostatin A on In vitro Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer
Histone Acetylation Porcine Embryo Somatic Cell Nuclear Transfer Trichostatin A
2016/5/16
Many different approaches have been developed to improve the efficiency of animal cloning by somatic cell nuclear transfer (SCNT), one of which is to modify histone acetylation levels using histone de...
Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells
Canine Interspecies Porcine Somatic cell Nuclear Transfer
2016/5/5
Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In ca...
Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits
Nuclear Transfer Oocyte Rabbit Follicle Somatic Cell
2016/5/3
The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients ...
Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer
Oviductal Fluid Nuclear Transfer Culture Media Zona Pellucida Porcine
2016/4/29
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
Relationship between Developmental Ability and Cell Number of Day 2 Porcine Embryos Produced by Parthenogenesis or Somatic Cell Nuclear Transfer
Blastomeric Fragmentation Developmental Ability Embryo Quality Parthenogenesis Porcine Somatic Cell Nuclear Transfer
2016/4/29
In vitro produced porcine embryos have potential application in reproductive biotechnology. However, their development potential has been very low. This study evaluated the in vitro developmental abil...
Effects of Donor Cell Passage, Size and Type on Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer
Donor Cell Passage Size Nuclear Transfer Porcine
2016/4/29
The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductal epithel...
Production of Bovine Nuclear Transfer Embryos Using Fibroblasts Transfected with Single-Chain Human Follicle-Stimulating Hormone Gene
Single-chain hFSH Transgenic Nuclear Transfer Cow
2016/4/29
Human follicle-stimulating hormone (hFSH) is a pituitary glycoprotein that regulates follicular development and ovulation. Clinically, hFSH has been used to induce follicular growth in infertile women...
In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
Murine Bovine Interspecies Somatic Cell Nuclear Transfer b-Mercaptoethanol Xist
2016/4/28
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studie...
核移植与治疗性克隆--Nuclear Transfer and Therapeutic Cloning
核移植 克隆 胚胎干细胞 治疗性克隆 Nuclear transfer Cloning Embryonic germ cells Therapeutic cloning
2007/12/5
核移植与治疗性克隆在畜牧业生产以及生物医学上具有广阔和诱人的应用前景。文章分析指出卵母细胞质量与供核细胞重新编程是影响体细胞核移植效率及克隆动物异常的主要因素，阐述了治疗性克隆所面临的一些基本问题及出路：治疗性克隆以核移植技术为基础，核移植所面临的一些问题也直接影响着治疗性克隆的临床应用；核移植胚胎干细胞分离培养效率的高低以及向重要功能细胞定向分化是治疗性克隆的前提；成体干细胞可用于一些重大疾病的...
Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits
Nuclear Transfer Oocyte Rabbit Follicle Somatic Cell
2016/4/26
The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients ...
Effect of Oocyte Activation Regimens on Ploidy of Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts in Rabbit
Activation Nuclear Transfer Development Ploidy Rabbit
2016/4/25
Considerable attention has been focused on the cloning of mammalian embryos, as a consequence of poor development, in order to enhance the application of genetic engineering. Experiments were conducte...
Production of Cloned Korean Native Goat (Capra hircus) by Somatic Cell Nuclear Transfer
Goat Superovulation Oocyte Cloning Nuclear Transfer Telomere
2016/4/25
The objectives of the present study were to initiate cloning of Korean native goat by somatic cell nuclear transfer (NT) and to examine whether unovulated (follicular) oocytes can support the same dev...
Development of Bovine Nuclear Transfer Embryos Using Life-span Extended Donor Cells Transfected with Foreign Gene
Chromosomal Abnormality Foreign Gene Transfection Life-span Extended Donor Cell Somatic Cell Nuclear Transfer
2016/4/22
This study was performed to determine the developmental potentials of nuclear transfer (NT) embryos using life-span extended cells transfected with a foreign gene as donor cells. A life-span extended ...