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Preparation of Epithelial and Stromal Cell Fractions from Immature Rat Prostatic Tissue Using Percoll Gradients
prostate androgens testosterone
2009/2/19
A method is described for the dispersion, isolation, and partial characterization of epithelial and stromal tissue cells from the rat ventral prostate. Viable epithelial and stromal cells have bee...
Endogenous Steroids in the Rat Seminiferous Tubules. Comparison of the Stages of the Epithelial Cycle Isolated by Transillumination-Assisted Microdissection
androgens seminiferous tubules rats stages of spermatogenesis transillumination
2009/2/19
A microdissection procedure is described that allows the isolation of segments of rat seminiferous tubules in different stages of the epithelial cycle. This procedure is based on spermatogenic sta...
The Effects of Experimental Cryptorchidism on the Entry of [3H]-Inulin and [3H]-Horseradish Peroxidase into the Lumen of the Rat Seminiferous Tubules
cryptorchidism blood-testis barrier rat testicle seminiferous tubule
2009/2/19
The objective of this research was to determine if experimental cryptorchidism causes significant changes in the rat blood-testis barrier. Micropuncture of the seminiferous tubules of normal, sham...
Low-molecular-weight Inhibin from Sheep, Human, Rat, and Chicken Testes
inhibin testes avian mammalian
2009/2/19
Low-molecular-weight peptides with inhibin activity have been isolated from sheep, human, rat, and chicken testes by simple gel filtration. These peptides, isolated from avian and mammalian specie...
The Effects of Cyclic Adenine Nucleotides, Phosphodiesterase Inhibitors, and Cauda Epididymidal Fluid on the Motility of Rat Epididymal Spermatozoa
spermatozoa motility epididymal rat
2009/2/19
Experiments were performed to determine whether the rat epididymis secretes a "forward-motility" factor(s) similar to that found by others in the bovine epididymis. Lumen content of the rat caput ...
Inhibition of Spermatogenesis and Steroidogenesis During Long-Term Treatment with hCG in the Rat
hCG spermatogenesis steroidogenesis
2009/2/19
The effects of chronic treatment with hCG (100 I.U.s.c. every second day) on testicular morphology, LH receptor levels and concentration of steroid intermediates of the 4 and 5 biosynthetic pathwa...
Testis Vein Testosterone Falls in the Aging Rat：Refutation of“Dilution”Hypothesis
dilution hypothesis in aging testicular function ratio of testicular vein to peripheral serum testosterone
2009/2/19
A proposal that serum testosterone concentration declines during aging In the male laboratory rat because it is diluted in a larger body mass (and larger blood plasma volume) was reexamined. Five ag...
In Vitro Effect of Inhibin on Cyclic AMP-Phosphodiesterase Activity in Rat Testes
inhibin cyclic AMP-phosphodiesterase
2009/2/19
We have previously reported that incubation of testicular slices with inhibin in the presence of 6 x 10-8 M oFSH resulted in a dose-related reduction in accumulation of cyclic AMP due to a decreas...
Turnover of Monocytoid Cells Within the Limiting Membrane of Rat Seminiferous Tubules
testis seminiferous tubules limiting membrane mononuclear leukocyte cell renewal
2009/2/19
The mode of renewal of mononuclear leukocytes found between the myoid cells forming the limiting membrane of rat seminiferous tubules was investigated by histometric methods. The average number of...
Effect of Estradiol on the Rat Prostate in the Presence and Absence of Testosterone and Pituitary
prostate rat estradiol testosterone pituitary prolactin
2009/2/19
In an attempt to elucidate the effect of estradiol on the rat prostate, four experiments were conducted. In experiment 1, rats were orchiectomized and were given subcutaneous implants of silastic ...
A Rapid and Simple Method for Isolating Principal Cells from the Rat Caput Epididymidis
epididymis principal cell rapid isolation
2009/2/19
A technique is described for the rapid isolation of principal cells from enzymatically dispersed rat caput epididymidis by differential sedimentation into calf serum. Sedimentations were prepared in...
Sperm Motility and Histomorphometry of the Testis of the Genetically Obese Zucker Rat
Zucker rat testis sperm
2009/2/19
The reproductive system of male obese Zucker rats was studied to determine whether there are abnormalities in testis composition, organ weights, or sperm motility, which might contribute to their se...
Rat Testicular Phospholipase A2 Activity：pH Optima, Its Cellular and Subcellular Distribution in the Gonad, and Some Factors That May Modulate Its Activity
rat testicular phospholipase A2 activity pH optima localization control
2009/2/19
A sensitive radiometric assay was developed for detecting phospholipase A1 and A2 (PLA1 and A2) activity. Four to five times more PLA2 activity was observed in rat testes than PLA1 activity. Two PLA...
Prolactin Stimulation of Testicular Steroid Biosynthesis in the Male Rat
prolactin LHRH 17-hydroxylase desensitization steroidogenesis LH receptor prolactin receptor
2009/2/19
The effect of prolactin on testicular steroidogenesis was studied in intact adult male rats and in animals treated for 12 days with the LHRH-agonist [D-Ala6, des-Gly-NH210]LHRH ethylamide (LHRH-agon...
Aging in the AXC Rat：Androgen Regulation of Prostate Prolactin Receptors
aging rat prostate receptors prolactin
2009/2/18
Prolactin receptor content of ventral prostates of young mature (4-5-month-old), adult (12-13-month-old), and aged (24-27-month-old) untreated AXC rats was 677 ± 151, 826 ± 242, and 213 ± 59 (P < 0.01...