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Time course of spontaneous in vitro sperm acrosome reaction
Capacitation fertility potential
2009/2/17
The acrosome reaction (AR) is an exocytotic process essential for sperm penetration of the zona pellucida and binding to the oocyte (DeJonge, 1994). Evaluation of in vitro AR can suggest fertility p...
Chromatin structural changes in sperm after scrotal insulation of Holstein bulls
Heat stress bovine sperm chromatin semen quality
2009/2/17
The reported effects on semen quality ascribed to testicular heat stress generally relate to traits impacting sperm transport and fertilizing ability but not to the genetic material contained by the...
The impact of reactive oxygen species on bovine sperm fertilizing ability and oocyte maturation
Spermatozoa fertilization development xanthine oxidase
2009/2/17
The objective of this study was to examine the effects of reactive oxygen species (ROS) on bovine sperm function and on the developmental competence of in vitro-matured bovine oocytes. In a first se...
Successful treatment of severe oligozoospermia with sperm washing and intrauterine insemination
Sperm processing artificial insemination male infertility
2009/2/17
During the period January 1, 1991 through December 31, 1995, 258 patients, in whom motile sperm counts for insemination (postwash, processed) were 10.0 million motile sperm or less were seen in the ...
The effect of follicle-stimulating hormone therapy on sperm quality:an ultrastructural mathematical evaluation
follicle-stimulating hormone therapy sperm quality ultrastructural mathematical evaluation
2009/2/17
We investigated the effect of follicle-stimulating hormone (FSH) administration on the ultrastructure of spermatozoa in order to evaluate the potential of FSH therapy for improving sperm quality. Fo...
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry
Sperm concentration cellular viability SYBR-14
2009/2/17
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull...
Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations:results of two fertility trials
Semen analysis CASA spermatozoa
2009/2/17
Two fertility trials were undertaken to evaluate the relationship between boar semen quality and fertility (conception rate and litter size) after on-farm artificial insemination (AI). Trial 1 inclu...
Effects of heat stress on mouse testicular cells and sperm chromatin structure
Testicular heat stress SCSA
2009/2/17
Scrotal regions of mice were exposed to a 38.0, 40.0, or 42.0 degrees C (+/-0.1) H2O bath for 60 minutes to determine the effects of elevated temperatures on testicular cells and sperm chromatin str...
Computerized imaging and scanning electron microscope(SEM)analysis of co-cultured fresh and frozen bovine sperm
Acrosome sperm computerized imaging scanning electron microscopy oviduct
2009/2/17
Fresh and frozen-thawed bull sperm were incubated with bovine oviductal epithelial cells and segments from the oviducts to examine the usefulness of these culture systems to model sperm changes in...
Discriminant analysis indicates a single sperm protein(SP22)is predictive of fertility following exposure to epididymal toxicants
Epididymis biomarker
2009/2/17
In a previous study, we found that ethane dimethanesulphonate (EDS) compromised the fertilizing ability of proximal cauda epididymal sperm from the rat within 4 days of exposure, an effect that pers...
Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm
Macaca fascicularis semen capacitation fertilization
2009/2/17
Hyaluronic acid (HA) not only surrounds the zona pellucida as part of the cumulus matrix but also is present throughout the zona pellucida and the perivitelline space of many mammalian oocytes. Ho...
Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction
Nitric oxide synthase acrosome reaction
2009/2/17
A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We com...
Choice of operating conditions to minimize sperm subpopulation sampling bias in the assessment of boar semen by computer-assisted semen analysis
CASA computer semen analysis tracking semen
2009/2/16
The performance of a computer-assisted semen analysis system was evaluated for use with washed boar spermatozoa. Accuracy was tested using a computer graphics-generated series of spots moving along ...
Anti-bull sperm monoclonal antibodies:II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Anti-bull sperm monoclonal antibodies:I. Identification of major antigenic domains of bull sperm and manifestation of interspecies cross-reactivity
Anti-sperm antibodies antigen localization conserved
2009/2/16
The overall objective of this series of experiments is to generate immunological markers that may elucidate bull sperm surface changes in vitro. Here we report the initial experiments of the study, ...