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Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath
human sperm fibrous sheath proteins AKAP4 AKAP3 dysplasia
2008/12/26
Dysplasia of the fibrous sheath (DFS) is characterized by male infertility, asthenozoospermia, and morphologically abnormal flagella that possess a severely malformed fibrous sheath. In many cases, DF...
The cyclic GMP-specific phosphodiesterase inhibitor, sildenafil, stimulates human sperm motility and capacitation but not acrosome reaction
cyclic GMP-specific phosphodiesterase inhibitor sildenafil human sperm motility capacitation
2008/12/25
Capacitation is the series of transformations that spermatozoa undergo in the female genital tract in order to bind to the zona pellucida, initiate the acrosome reaction, and fertilize an egg. Cyc...
Functional and ultrastructural features of DNA-fragmented human sperm
DNA-fragmented human sperm
2008/12/25
The functional significance of deoxyribonucleic acid (DNA) fragmentation in ejaculated human sperm is unclear. In this study the extent of DNA strand breakage in swim-up selected spermatozoa was eva...
Effect of pentoxifylline on the intrinsic swimming forces of human sperm assessed by optical tweezers
pentoxifylline intrinsic swimming forces human sperm optical tweezers
2008/12/25
It is still controversial whether in vitro exposure of sperm to pentoxifylline increases sperm motility and force, which is defined as the product of velocity by beat frequency of the tail. Laser ...
Characteristics of human sperm chromatin structure following an episode of influenza and high fever：a case study
Characteristics human sperm chromatin structure episode influenza high fever
2008/12/25
Semen samples from a fertile patient presenting with influenza and a 1-day fever of 39.9 degrees C were obtained and analyzed at 18-66 days postfever (dpf) for sperm nuclear proteins, DNA stainabi...
Cholesterol inhibitory effects on human sperm-induced acrosome reaction
Cholesterol inhibitory human sperm-induced acrosome reaction
2008/12/25
Progesterone (P4) is known to induce an acrosome reaction in mammalian sperm in vitro, whereas cholesterol is a major inhibitor of acrosome reaction. This study had three objectives: to study the in...
First Steps in the Development of a Functional Assay for Human Sperm Using Pig Oocytes
Sperm functionality assay species specificity sperm-oocyte interaction heterologous ICSI
2008/12/24
The use of mammalian oocytes to assess human sperm functionality could be a helpful tool with potential applications in clinical and research programs. In an attempt to develop the pig model, the...
Comparison of Chromatin Assays for DNA Fragmentation Evaluation in Human Sperm
SCSA TUNEL SCD AOT
2008/12/23
Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring. We evaluated chromatin integrity in sperm from 60 infertile men and 7 fertile don...
The objective of this study was to examine the relationship between DNA integrity and protamines in human sperm. One hundred forty-nine male infertility patients were included in an Institutional Re...
Electrophoretic Characterization of the Human Sperm–Specific Enolase at Different Stages of Maturation
Human spermatozoa maturation enolase isoforms epididymal sperm testicular sperm
2008/12/22
The presence of a sperm-specific enolase isoform (ENO-S) in human ejaculated spermatozoa was previously demonstrated. The objective of this study was to characterize this ENO-S in spermatozoa at dif...
Annexin V Binding and Merocyanine Staining Fail to Detect Human Sperm Capacitation
FACScan membrane architecture phosphatidylserine translocation
2008/12/22
The signaling pathways that characterize the process of capacitation of human spermatozoa are still largely unknown. Modifications in the lipid architecture of the sperm plasma membrane have been de...
Why the WHO Recommendations for Eosin-Nigrosin Staining Techniques for Human Sperm Vitality Assessment Must Change
Recommendations Eosin-Nigrosin Staining Human Sperm Vitality Assessment
2008/12/22
In basic semen analysis and many research-oriented investigations, it is important to distinguish between dead and live spermatozoa. A proportion of live spermatozoa can be identified by their motilit...
Role of Nitric Oxide Concentrations on Human Sperm Motility
Male infertility spermatozoa motility asthenozoospermia
2008/12/22
Nitric oxide (NO) is a free radical generated from the oxidation of L-arginine to L-citrulline by 3 isoforms of reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent NO synthases. Se...
Identification of Glutamate Receptors and Transporters in Mouse and Human Sperm
Uptake immunocytochemistry RT-PCR inhibitor
2008/12/22
-Aminobutyric acid (GABA) and glutamate (Glu) are considered as the predominant inhibitory and excitatory neurotransmitters in mammalian central nervous systems (CNS), respectively. The presence o...
Lack of Voltage-Dependent Calcium Channel Opening During the Calcium Influx Induced by Progesterone in Human Sperm. Effect of Calcium Channel Deactivation and Inactivation
Intracellular calcium mammalian sperm membrane potential membrane potential-sensitive dyes
2008/12/22
Progesterone induces calcium influx and acrosomal exocytosis in human sperm. Pharmacologic evidence suggests that voltage-dependent calcium channels (VDCCs) are involved. In this study, membrane p...