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Effect of low-dose testicular irradiation on sperm count and fertility in patients with testicular seminoma
Testicular cancer radiation treatment sperm banking
2009/2/13
The treatment of seminoma with radiation therapy risks transient infertility. We have prospectively followed eight patients with stage I seminoma of the testicle. All patients underwent radical or...
Two-dimensional electrophoretic profile of human sperm membrane proteins
Human spermatozoa membrane proteins two-dimensional electrophoresis contraceptive vaccine
2009/2/13
The purpose of this study was to characterize highly enriched human spermatozoa membrane proteins by two-dimensional electrophoresis and computer image analysis. Sperm membrane proteins were extract...
Phosphatidylcholine enhances the acrosomal responsiveness of human sperm
Phosphatidylcholine enhances acrosomal responsiveness human sperm
2009/2/12
Supplementing bovine serum albumin-containing medium with phosphatidylcholine (PC) accelerated the in vitro development of human sperm acrosomal responsiveness. Responsiveness was assessed by expo...
Effect of bovine oviductal estrus-associated protein on the ability of sperm to capacitate and fertilize oocytes
Oviduct culture proteinsecretion oviductal fluid
2009/2/12
At estrus, an oviduct-specific, estrus-associated glycoprotein (EAP) of molecular weight 85-95,000, is secreted by the oviductal epithelium and found in cannula-derived bovine oviductal fluid (ODF). T...
Nanovid microscopy for assessing sperm membrane changes induced by in vitro capacitating and acrosomal reacting procedures
Spermatozoa colloidal gold videomicroscopy
2009/2/12
This study was to verify the usefulness of Nanovid microscopy techniques for evaluating induced modifications in bovine spermatozoal membranes. Frozen thawed bovine sperm were labeled with 20-nm c...
Development of computer-directed methods for the identification of hyperactivated motion using motion patterns developed by rabbit sperm during incubation under capacitation conditions
computer-directed methods identification hyperactivated motion motion patterns rabbit sperm
2009/2/12
Rabbit spermatozoa developed motions that mimicked hyperactivated motility during incubation for 16-20 hours under capacitation conditions and in several other commonly used media. Sperm from some rab...
Leukocytic infiltration into the human ejaculate and its association with semen quality, oxidative stress, and sperm function
Leukocytes reactive oxygen species sperm function
2009/2/12
Immunocytochemical techniques have been used to monitor the size and composition of the leukocyte population in unfractionated human semen samples and sperm populations generated by Percoll gradie...
A morphometric comparison of the nuclear morphology of fresh and frozen-thawed human zona-bound and unbound sperm
Sperm nucleus computer-assisted morphometry zona binding cryopreservation DNA
2009/2/12
The role of sperm nuclear morphology in zona binding and the effect of cryopreservation on sperm nuclear morphology have been investigated using objective criteria. The nuclear dimensions of fresh a...
Effect of follicular or oviductal fluids on movement characteristics of bovine sperm during capacitation in vitro
Sperm motion analysis motility hyperactivation
2009/2/12
Mammalian sperm exhibit characteristic motility changes associated with capacitation. Movement characteristics of bovine sperm incubated in noncapacitating (control, medium alone), capacitating (ovidu...
The ethane dimethanesulfonate-induced decrease in the fertilizing ability of cauda epididymal sperm is independent of the testis
Epididymis sperm maturation in utero insemination fertilization toxicant
2009/2/12
Several decades ago it was reported that when adult male rats were exposed to a single injection of 50 mg/kg body weight ethane dimethanesulfonate (EDS) and mated with untreated females, average l...
Tunica vaginalis sperm reservoir in a monkey model of vas deferens obstruction
Vas deferens spermatozoa epididymis male infertility
2009/2/12
Repeat microscopic epididymal sperm aspiration (MESA) is used to treat congenital absence of the vas deferens (CAVD). Use of alloplastic implants as reservoirs results in poor sperm motility and pregn...
Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation
superoxide dismutase loss human sperm cells cryopreservation
2009/2/12
Earlier studies on human sperm cryodamage have shown that plasma membrane stress is the primary process and that phospholipid peroxidation in cryopreserved samples is not inhibited by addition of an...
Hemizona index（HZI）demonstrates excellent predictability when evaluating sperm fertilizing capacity in in vitro fertilization patients
IVF predictability Hemizona assay IVF
2009/2/12
The objective of this work was to assess the power of the hemizona assay to predict in vitro fertilization (IVF) results using receiving operating characteristics (ROC) analysis. In a tertiary medical...
Interleukin-6 enhances the fertilizing capacity of human sperm by increasing capacitation and acrosome reaction
Interleukin-6 enhances human sperm acrosome reaction
2009/2/12
The effects of human recombinant interleukin-6 (IL-6) on human sperm penetration of zona-free hamster ova (SPA) and human sperm cell capacitation and acrosome reaction were investigated. IL-6 at h...
The detrimental effects of electric current on normal human sperm
Human sperm electric current/temperature motility
2009/2/12
This in vitro study was conducted to determine the direct effects of electric current and associated increase in temperature on human sperm. Washed sperm samples from normal volunteers were subjecte...