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Analysis of promoter and androgen regulatory sequences required for optimal transcription of the rat androgen-binding protein gene
Androgen-binding protein (ABP) sex hormone-binding globulin (SHBG) Sertoli cells transcriptional regulation
2009/2/17
The androgen-binding protein (ABP) gene P1 promoter directs cell-specific gene regulation of ABP secreted by Sertoli cells. A recent study using the mouse Sertoli cell line (MSC-1) with a luciferase...
Suppression and recovery of spermatogenesis following spinal cord injury in the rat
Sertoli cells Leydig cells testosterone follicle stimulating hormone
2009/2/17
Recently, we reported that changes in spermatogenesis in adult rats during acute phase (within 2 weeks) of spinal cord injury (SCI) were associated with a suppression of pituitary-testis hormone a...
Rat testicular germ cells and epididymal sperm contain active P450 aromatase
Estrogen testis epididymis
2009/2/17
Although testosterone is the principal sex steroid produced by the testis, estrogen is known to be produced by both Leydig and Sertoli cells during different developmental periods. Additionally, evide...
Transforming growth factor-beta1（TGF-beta1）in penile and prostate growth in the rat during sexual maturation
Penis androgen receptors
2009/2/17
The goal of this study was to determine whether transforming growth factor-beta1 (TGF-beta1) may contribute to the arrest of penile growth and the down-regulation of androgen receptors (AR) that occ...
Androgen regulation of the Pem homeodomain gene in mice and rat Sertoli and epididymal cells
Homeobox testosterone promoter testis
2009/2/17
Although the role of homeodomain transcription factors during embryogenesis is well known, their developmental function in postnatal animals is only beginning to be understood. We examined the regul...
Effect of chorionic gonadotropin and flutamide on Leydig cell and macrophage populations in the testosterone-estradiol-implanted adult rat
chorionic gonadotropin Leydig cell macrophage populations testosterone-estradiol-implanted adult rat
2009/2/17
The objective of this study was to investigate the role of androgens and nonandrogenic Leydig cell products in maintaining Leydig cell and macrophage numbers in the testis of the adult rat. Adult ...
Haptoglobin is a Sertoli cell product in the rat seminiferous epithelium：its purification and regulation
Germ cell testis
2009/2/17
Using multiple HPLC steps, a protein of 67 kDa (estimated by gel permeation HPLC) was purified from Sertoli cell-enriched culture medium that consisted of two dissimilar subunits of 9 (alpha chain...
Estrogen receptor（alpha and beta）expression in the excurrent ducts of the adult male rat reproductive tract
Efferent ductules immunohistochemistry
2009/2/17
The understanding of estrogen's function in the male reproductive tract is limited, and estrogen receptor (ER) localization in the reproductive tract of the adult male rat has not been described. ...
Androgenic maintenance of the rat erectile response via a non-nitric-oxide-dependent pathway
Penile erection testosterone cGMP phosphodiesterase
2009/2/17
Prior studies have demonstrated that the erectile response in the rat penis is androgen dependent and is mediated by nitric oxide (NO), the neurotransmitter synthesized by the enzyme nitric oxide ...
Mild hypothermia induces apoptosis in rat testis at specific stages of the seminiferous epithelium
Cell death spermatogenesis ferti
2009/2/17
This study was designed to determine the effects of short episodes of mild hypothermia on cell apoptosis in the testis of the adult rat. Apoptosis was assessed by in situ DNA 3'-end labeling in the ...
Stability and transcriptional regulation of gamma-glutamyl transpeptidase mRNA expression in the initial segment of the rat epididymis
Stability transcriptional regulation gamma-glutamyl transpeptidase mRNA initial segment rat epididymis
2009/2/17
Gamma-glutamyl transpeptidase (GGT) is an enzyme believed to play a role in the protection of maturing spermatozoa in the epididymis. Our previous studies have shown that four GGT mRNAs (I-IV) trans...
The effects of gonadotropin-releasing hormone immunization and recombinant follicle-stimulating hormone on the Leydig cell and macrophage populations of the adult rat testis
Stereology morphometry optical disector leucocytes
2009/2/17
The objective of this study was to investigate the role of the gonadotropins and, in particular follicle-stimulating hormone (FSH) in maintaining the Leydig cell and macrophage populations of the adul...
Development and characterization of a prepubertal rat Sertoli cell line, 93RS2
Tissue culture cell line transferrin SGP-1 SGP-2 SV4O temperature-sensitive large T malignancy
2009/2/17
Sertoli cells in the seminiferous epithelium provide both structural and nutritional support to germ cells during spermatogenesis. Primary Sertoli cells in culture are an effective tool for the in v...
Localization and effects of calcitonin gene-related peptide in the testicular vasculature of the rat
CGRP testis blood flow vasomotion
2009/2/17
Using laser Doppler flowmetry, the effects of unilateral intratesticular injection of calcitonin gene-related peptide (CGRP) and CGRP8-37, a CGRP-receptor antagonist, on right- and left-testicular b...
Differential methylation in steroid 5 alpha-reductase isozyme genes in epididymis, testis, and liver of the adult rat
Gene methylation gene expression androgens dihydrotestosterone eukaryotic adenine methylation
2009/2/17
DNA methylation has been largely involved in the regulation of tissue-specific gene expression. The aim of the study was to determine the methylation pattern of steroid 5 alpha-reductase genes 1 and 2...