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Synchronous assessment of sperm motility and fertilizing ability in the hamster following treatment with alpha-chlorohydrin
Synchronous assessment sperm motility fertilizing ability
2009/2/13
To investigate the relationship between sperm motion parameters and fertilizing ability, a model was developed to assess both of these endpoints synchronously using a toxicant that inhibits sperm mo...
Structure of the rRNA genes in the hamster sperm nucleus
Nuclear matrix nucleolus DNA structure DNA loop
2009/2/13
We have examined the structure of the major ribosomal RNA (rRNA) genes in the hamster sperm nucleus, using fluorescent in situ hybridization (FISH). The rRNA genes are present as tandemly repeated...
Sperm immobilizing activity of a synthetic bioactive peptide 20-44 of 37-kDa cationic antimicrobial protein（CAP37）of human neutrophils
Amphipathic peptides antisperm antibodles complement neutrophil granule-associated protein
2009/2/13
We have previously reported that human sperm coincubated with human peripheral blood neutrophils in the presence of complement (C)-fixing antisperm antibody (ASA)-positive sera are rapidly interna...
Low levels of nitric oxide promote human sperm capacitation in vitro
NONOates reactive oxygen species catalase hydrogen peroxide peroxinitrite nitric oxide synthase
2009/2/13
The influence of nitric oxide on human sperm hyperactivation and capacitation, as well as its mechanism of action and its possible origin from spermatozoa were studied. Percoll-washed spermatozoa ...
Notulae seminologicae. 5. Mathematical evaluation of interdependent submicroscopic sperm alterations
Seminology electron microscopy mathematical formula Bayes theory sperm pathology human infertility
2009/2/13
This paper concerns the mathematical evaluation of sperm quality as examined by electron microscopy. Proceeding with a Bayesian technique, we have developed a formula considering all statistical pos...
Effects of glucose and other energy substrates on the hyperactivated motility of macaque sperm and the zona pellucida-induced acrosome reaction
glucose hyperactivated motility macaque sperm
2009/2/13
Energy sources in sperm capacitation media have various effects on mammalian sperm and are required for stimulation of hyperactivated motility and/or acrosome reactions in some species. The presen...
Functional characterization of the primate sperm acrosomal antigen（PSA-63）
Functional characterization primate sperm acrosomal antigen
2009/2/13
Monoclonal antibody (HS-63) raised in mice against human ejaculated sperm, polyclonal antibodies raised in rabbits against the cognate mouse testicular antigen (MSA-63; or Fab) and polyclonal antibodi...
Importance of bicarbonate to the progesterone-initiated human sperm acrosome reaction
Anion channel bicarbonate sperm acrosome reaction
2009/2/13
Progesterone, a putative in vivo initiator of the human sperm acrosome reaction (AR), has previously been shown to act at the sperm plasma membrane to initiate the AR in vitro. Here, we have investi...
Comparative study of boar sperm coming from the caput, corpus, and cauda regions of the epididymis
boar sperm caput corpus cauda regions epididymis
2009/2/13
Sperm quality in the caput, corpus, and cauda regions of the epididymis of healthy and sexually mature Landrace boars was studied. Epididymal sperm characteristics were examined by light microscopy (L...
Differential responses of human sperm to varying concentrations of pentoxyfylline with demonstration of toxicity
human sperm pentoxyfylline demonstration of toxicity
2009/2/13
Pentoxyfylline (PF), a methylxanthine derivative, is an inhibitor of the cAMP-phosphodiesterase enzyme, and is known to stimulate the motility of fresh and post-thaw human sperm. The purpose of th...
New methods of specimen preparation were developed and a new method of objective, automated sperm morphometry analysis (ASMA) was performed to reduce the technical variation in the rabbit sperm mo...
Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay
Acrldine orange chromatin structure SCSA TdTA
2009/2/13
Sperm from four mammalian species were analyzed by the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase assay (TdTA) using flow cytometry. The SCSA quantitates the ...
Expression of mannose-binding sites on human spermatozoa and their role in sperm-zona pellucida binding
mannose-binding sites human spermatozoa sperm-zona pellucida binding
2009/2/13
A D-mannosylated albumin (DMA) neoglycoprotein was assessed to validate experimentally a probe capable of detecting mannose-binding sperm receptors involved in human sperm-egg interaction. DMA spe...
Characterization of two second messenger pathways and their interactions in eliciting the human sperm acrosome reaction
Signal transduction protein kinase A protein kinase C crosstalk acrosome reaction
2009/2/13
The human sperm acrosome reaction (AR) occurs via the activation of at least two signal transduction pathways. The purpose of this investigation was to characterize two of the pathways, the protei...
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide
Sperm fluorescence SYBR-14 flow cytometry vital staining cryopreservation
2009/2/13
A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI...