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Ribonuclease S Dynamics Measured Using a Nitrile Label with 2D IR Vibrational Echo Spectroscopy
Anthraquinonic dyes Boron-doped diamond anode Metal catalysts Solar photoelectro-Fenton Wastewater treatment
2016/5/25
A nitrile-labeled amino acid, p-cyanophenylalanine, is introduced near the active site of the semisynthetic enzyme ribonuclease S to serve as a probe of protein dynamics and fluctuations. Ribonuclease...
Quantitative,Directional Measurement of Electric Field Heterogeneity Within an Enzyme Active Site
Quantitative Directional Measurement Electric Field Heterogeneity Enzyme Active Site
2016/5/25
Extensive structural studies of enzymes have revealed that biological catalysis occurs within sequestered active site crevices, where reacting substrates are surrounded by an anisotropic and chemicall...
Evaluation of the Energetics of the Concerted Acid-Base Mechanism in Enzymatic Catalysis:The Case of Ketosteroid Isomerase
Energetics Concerted Acid-Base Mechanism Enzymatic Catalysis Ketosteroid Isomerase
2016/5/25
Structures of enzymes invariably reveal the proximity of acidic and basic residues to reactive sites on the substrate, so it is natural and common to suggest that enzymes employ concerted mechanisms t...
Vesicle Fusion Observed by Content Transfer Across a Tethered Lipid Bilayer
Endoplasmic reticulum-associated degradation (ERAD) ubiquitin-proteasome system (UPS) gp78/RNF45 Epidermal growth factor receptor (EGFR) Exosome Duffy antigen receptor for chemokine (DARC)
2016/5/25
Synaptic transmission is achieved by exocytosis of small, synaptic vesicles containing neurotransmitters across the plasma membrane. Here, we use a DNA-tethered freestanding bilayer as a target archit...
Direct Measurement of the Protein Response to An Electrostatic Perturbation That Mimics the Catalytic Cycle in Ketosteroid Isomerase
hydroperoxide lipids GUV photosensitization methylene blue
2016/5/25
Understanding how electric fields and their fluctuations in the active site of enzymes affect efficient catalysis represents a critical objective of biochemical research. We have directly measured the...
Nitrile Bonds as Infrared Probes of Electrostatics in Ribonuclease S
Nitrile Bonds Infrared Probes Electrostatics Ribonuclease S
2016/5/25
Three different nitrile-containing amino acids, p-cyanophenylalanine, m-cyanophenylalanine, and S-cyanohomocysteine, have been introduced near the active site of the semisynthetic enzyme ribonuclease ...
Decomposition of Vibrational Shifts of Nitriles into Electrostatic and Hydrogen-Bonding Effects
Pseudomonas putida Nitriles Steroid Isomerases Spectrophotometry, Infrared Magnetic Resonance Spectroscopy Catalytic Domain Hydrogen Bonding Vibration Static Electricity
2016/5/25
Infrared (IR) band shifts of isolated vibrational transitions can serve as quantitative and directional probes of local electrostatic fields, due to the vibrational Stark effect. However, departures f...
Solvation Response Along the Reaction Coordinate in the Active Site of Ketosteroid Isomerase
Pseudomonas putida Coumarins Isomerases Spectrometry, Fluorescence Catalytic Domain Ketosteroids Biocatalysis Light Models, Molecular Equilenin
2016/5/25
A light-activated reaction analog has been developed to mimic the catalytic reaction cycle of Delta(5)-3-ketosteroid isomerase to probe the functionally relevant protein solvation response to the cata...
Proton Affinity of the Oxyanion Hole in the Active Site of Ketosteroid Isomerase
Pseudomonas putida Protons Steroid Isomerases Crystallography,X-Ray Nuclear Magnetic Resonance,Biomolecular Catalytic Domain Protein Conformation Substrate Specificity Ketosteroids Mutagenesis, Site-Directed
2016/5/25
The absorption spectra of a series of inhibitors bound at the active site of Delta(5)-3-ketosteroid isomerase from Pseudomonas putida were found to exhibit substantial variations in the contributions ...
Charge Transfer in Photoacids Observed by Stark Spectroscopy
cre recombinase pituitary GSU transgenic mouse muscle
2016/5/25
The charge redistribution upon photoexcitation is investigated for a series of pyrene photoacids to better understand the driving force behind excited-state proton-transfer processes. The changes in e...
Electrostatic Fields Near the Active Site of Human Aldose Reductase:1.New Inhibitors and Vibrational Stark Effect Measurements
apoptosis ATM cisplatin IKK p73 ubiquitination
2016/5/25
Vibrational Stark effect spectroscopy was used to measure electrostatic fields in the hydrophobic region of the active site of human aldose reductase (hALR2). A new hALR2 inhibitor was designed and sy...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.2.Unusual Photophysical Properties
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e
2016/5/25
In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, an...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.1.Mutagenesis and Structural Studies
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e.
2016/5/24
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
Energetics of Primary Charge Separation in Bacterial Photosynthetic Reaction Center Mutants:Triplet Decay in Large Magnetic Fields
horseradish peroxidase lignin peroxidase enzymatic oxidations N-demethylation methylene blue azure B azure C
2016/5/24
The triplet state of aromatic molecules forms and decays by intersystem crossing, as originally demonstrated by Kasha and Lewis. By contrast, the triplet state of the primary electron donor, 3 P, in p...
Fluorescence Lifetimes Measurements Using a Synchrotron Source
Fluorescence Lifetimes Measurements Synchrotron Source
2016/5/20
Fluorescence Lifetimes Measurements Using a Synchrotron Source.