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Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
bovine viral diarrhea virus RNA real-time RT-PCR SYBR Green I
2015/3/13
Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR wa...
Development of Mass Spectrometry-Based Methods for Quantitation and Characterization of Protein Drugs: Transferrin as a Model Drug Delivery Vehicle
Mass Spectrometry O18 labeling Protein quantitation Transferrin
2014/11/20
In the last two decades, protein drugs have enjoyed a rapid growth and achieved a tremendous success in treating human diseases. However, the presence of physiological barriers greatly impedes the eff...
Quantitation of Neuronal Loss Induced by Status Epilepticus and the Effects of Nitric Oxide
Lithium-pilocarpine tatus epilepticus L-Arginine -NAME Neuron loss
2009/6/30
Neuronal loss in the hippocampal CA1 and CA3 fields due to lithium-pilocarpine- induced status epilepticus (SE) and the effects of a nitric oxide (NO) substrate, L-Arginine and a NO synthase inhibitor...
Quantitation of Carotenoids in Raw and Processed Fruits in Japan
carotenoid fruit phytoene
2009/6/10
To obtain the quantitative and qualitative data available for estimating the intake of carotenoids from fruits in Japan, carotenoids were analyzed with reversed phase high-performance liquid chromatog...
Quantitation of Carotenoids in Commonly Consumed Vegetables in Japan
vegetable carotene lycopene
2009/5/20
The carotenoid content, potentially an antioxidant, of 70 vegetables in Japan was determined by reversed-phase HPLC. α-Carotene was detected in 55 of 70 vegetable samples; β-carotene, in all 70; lycop...
Determination and Quantitation of Anthocyanins and Hydroxycinnamic Acids in Different Cultivars
sweet cherries (Prunus avium L.) anthocyanins hydroxycinnamic acids HPLC
2009/3/20
The anthocyanins and hydroxycinnamic acids in 5 cultivars of dark coloured sweet cherries were characterised and quantified by means of HPLC and UV-VIS spectrophotometry. Phenolic components were extr...
Acrosin, Proacrosin, and Acrosin Inhibitor of Human Spermatozoa：Extraction, Quantitation, and Stability
acrosin proacrosin acrosin inhibitor human spermatozoa
2009/2/18
Ten different methods were evaluated to determine the treatment for obtaining maximal amounts of acrosin, proacrosin, and acrosin inhibitor (the acrosin system) from human spermatozoa by a single te...
Hormonal regulation of spermatogenesis in the hypophysectomized rat: quantitation of germ-cell population and effect of elimination of residual testosterone after long-term hypophysectomy
Testis germ cells androgen morphometry
2009/2/17
Spermatogenesis continues after long-term hypophysectomy (Hx), but massive cell degeneration prevents seminiferous tubules from attaining the full complement of cells. One objective of this study ...
Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen
Spermatozoa antibody infertility
2009/2/10
Detection of sperm surface antibodies is important for the diagnosis and treatment of infertility. The authors have investigated the methodologic aspects of flow cytometry (FCM) to detect sperm-bo...
Quantitation of rotational movements associated with surgical mandibular advancement
Mandibular advancement Bilateral sagittal split osteotomy Opening rotation Curve of Spee
2009/1/14
Surgical mandibular advancement can be used to correct an anteroposterior and/or vertical malocclusion. The procedure of choice is often the bilateral sagittal split osteotomy (BSSO). By varying the...
Quantitation of Seminal Factor IX and Factor IXa in Fertile, Nonfertile, and Vasectomy Subjects：A Step Closer Toward Identifying a Functional Clotting System in Human Semen
Fertility novel finding.
2008/12/23
Coagulation factor (F) IX is a zymogen of the plasma serine proteases, one that plays an essential role in the regulation of normal blood coagulation. Congenital defects of FIX synthesis or functi...
(Review) Quantitation of Fiber Quality and the Cotton Production-Processing Interface: A Physiologist's Perspective
(Review) Quantitation Fiber Quality the Cotton Production-Processing Physiologist's Perspective
2008/12/15
Traditionally, ideal cotton (Gossypium ssp.) fibers are said to be as white as snow, as strong as steel, as fine as silk and as long as wool. It is difficult to incorporate these specifications favore...
Small-Sample Cotton Fiber Quality Quantitation
Quality Quantitation Small-Sample Cotton Fiber
2008/4/14
Cotton [Gossypium spp.] fiber quality quantitations at the boll, locule, or seed level are limited by the large sample-size requirements of commercial cotton fiber testing instrumentation and inherent...
Influence of Standards and Antibodies in Immunochemical Assays for Quantitation of Immunoglobulin G in Bovine Milk
bovine immunoglobulin G subclasses enzyme-linked immunosorbent assay
2008/3/27
This study reports the influence of using various reference antigens or standards as well as the source of antibody in the immunochemical quantitation of bovine immunoglobulin (Ig) G. Standard curves ...
A Novel Approach to the Quantitation of Coeluting Cantharidin and Deuterium Labelled Cantharidin in Blister Beetles (Coleop-tera: Meloidae)
Labelled cantharidin GC-MS/MS MRM
2010/4/27
Blister beetles (Coleoptera: Meloidae) are the main natural source of cantharidin, but the compound titre is depended on several factors including, age, sex and mating status of the insects. In order ...