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旨在研究雄性山羊主要繁殖器官中PRM1 mRNA表达特性，分析精子PRM1 mRNA表达与精液品质参数的相关性。本研究通过荧光定量PCR技术分析PRM1 mRNA表达规律，利用免疫组化技术对睾丸中PRM1蛋白进行定位，并应用GraphPad Prism 5软件分析精子PRM1 mRNA表达量与精液品质指标的相关性。结果显示，PRM1 mRNA在睾丸中高度表达，其次是附睾，睾丸中PRM1 mRNA表...
Polycationic protamine for water-insoluble complex formation with DNA
Protamine DNA DNA/protamine complex
2010/11/30
The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examin...
Expression of Protamine mRNA in Relation to Spermatogenic Activity in Japanese Quail
Protamine mRNA spermatogenesis quail short day treatment testis
2009/7/31
This study was conducted to elucidate relationship between testicular protamine mRNA expression and spermatogenic activity in the quail as the following experiments. (1) Quail were raised under contin...
Identification of Spermatogenic Cells Expressing Protamine mRNA in Japanese Quail by RT-PCR
micropipetting protamine mRNA quail RT-PCR spermatogenic cells
2009/7/31
This study was conducted by reverse transcription-polymerase chain reaction (RT-PCR) analysis to determine when the protamine mRNA is expressed in testicular germ cells during spermatogenesis in quail...
Altered protamine 2 expression is uncommon in donors of known fertility, but common among men with poor fertilizing capacity, and may reflect other abnormalities of spermiogenesis
Semen sperm chromatin fertilization IVF infertility
2008/12/29
During the spermatid elongation stage of spermiogenesis approximately 85% of sperm nuclear histones are replaced by protamines. Protamines increase the packing ratio of sperm chromatin, presumably fac...
Protamine Levels Vary Between Individual Sperm Cells of Infertile Human Males and Correlate With Viability and DNA Integrity
Spermatogenesis male fertility
2008/12/24
Sperm protamine deficiency has been associated with human male infertility. However, most studies have adopted a global approach to assessing sperm protamine levels. Thus, it is not known whether...
Sperm Nuclear Histone to Protamine Ratio in Fertile and Infertile Men：Evidence of Heterogeneous Subpopulations of Spermatozoa in the Ejaculate
Sperm DNA male infertility
2008/12/24
Sperm protamine deficiency is observed in a subset of infertile men, suggesting that the relative histone to protamine ratio may be altered in the spermatozoa of these men. We measured the ratio of ...
The objective of this study was to examine the relationship between DNA integrity and protamines in human sperm. One hundred forty-nine male infertility patients were included in an Institutional Re...
Marked Differences in Protamine Content and P1/P2 Ratios in Sperm Cells From Percoll Fractions Between Patients and Controls
Chromatin condensation male infertility Percoll gradients sperm chromatin sperm morphology sperm motility
2008/12/19
The various sperm cell types present in a simple ejaculate differ in their motility and morphology. However, little is known about the nuclear maturity of these sperm cells and their relationship wi...