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Effect of Mytomycin C Treatment on Feeder Cells and the Proliferation of Cultured Chicken Primordial Germ Cells Isolated from Embryonic Blood
chicken primordial germ cell culture feeder cell mytomycin C
2009/8/3
Chicken primordial germ cells isolated from embryonic blood were cultured in vitro on feeder cells derived from the gonads of 7-day incubated embryos. Primordial germ cells were proliferated about 13 ...
Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs)
chimeras germ line primordial germ cell embryos
2009/8/3
This experiment was designed to produce chimeric chicken by transferring primordial germ cells (PGCs) of Ehime native chicken embryos to White Leghorn ones. Fertilized eggs produced by both chicken br...
Population of Circulating Primordial Germ Cells in Early Japanese Quail Embryos
primordial germ cells circulation erythrocytes quail embryo
2009/8/3
Avian primordial germ cells (PGCs) show a unique migration pathway during early development. As soon as the blood vessels have formed, PGCs enter the circulatory system, and migrate to the gonadal pri...
In vitro Proliferation of Chick Primordial Germ Cells Co-cultured with Germinal Ridge Stroma Cells
chicken PGCs in vitro culture proliferation germinal ridge stroma cells
2009/8/3
In order to develop the technique for in vitro culture of chicken primordial germ cells obtained from circulating embryonic blood vessels (cPGCs), participation of feeder cells and some of growth fact...
Developmental Genetic Analysis of the Avian Primordial Germ Cells and the Applications for Production of Chimeric Chickens
Germ Cells Chimeric Chickens Avian
2009/7/31
Developmental Genetic Analysis of the Avian Primordial Germ Cells and the Applications for Production of Chimeric Chickens.
Genetic Factors Affect the Number of Circulating Primordial Germ Cells in Early Chick Embryos
primordial germ cells circulation chick embryo male female
2009/7/31
Avian primordial germ cells (PGCs) arise from the epiblast and circulate temporarily via the blood vascular system in their migration route to the gonadal anlage. There are egg-to-egg variations in th...
Conservation of a Genetically Selected Broiler Line (42L)Using Cryopreserved Circulating Primordial Germ Cells (PGCS) Isolated by Filtration Method
Filtration method PGCs cryopreservation germ-line chimeras chicken
2009/7/31
A genetically selected broiler line (42L) developed at the Pennsylvania State University, PA, USA was used in the present study. In Experiment 1, embryonic blood was collected from chick embryos at ...
Effects of Ultraviolet Irradiation on the Migratory Ability of Primordial Germ Cells (PGCs) in the Domestic Chicken
UV irradiation PGCs Migratory ability embryo chicken
2009/7/31
The effect of ultraviolet (UV) irradiation on the ability of primordial germ cells (PGCs) to migrate toward the gonadal ridge was investigated in the domestic chicken.
When PGCs were irradiated with ...
Effects of Soft X-Ray Irradiation to Stage X Blastoderm on Restriction of Proliferation of Primordial Germ Cells in Early Chicken Embryos
chick embryo primordial germ cell soft X-ray stage X blastoderm
2009/7/30
It is necessary to deplete the endogenous primordial germ cells (PGCs) from recipient embryos when germline chimaeric chickens are produced by the transfer of PGCs. In this study, effects of soft X-ra...
Conservation of a Threatened Indigenous Fowl (Kureko Dori) Using the Germline Chimeras Transplanted from Primordial Germ Cells
endangered species domestic fowl germline chimera primordial germ cells transplantation
2009/7/30
This is the first successful report on producing the endangered domestic fowl, Kureko Dori (KD), which is a Kumamoto prefectural natural treasure, using germline chimeras transplanted from primordial ...
Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo
gonads green fluorescent protein primordial germ cell transfection
2009/7/30
The present study was carried out to develop techniques for introducing exogenous DNA into primordial germ cells (PGCs) and expressing the introduced DNA efficiently in the gonads of developing chicke...
Effect of Transfer Primordial Germ Cells (PGCs) into Chick Gonad
chicken embryo gonad PGCs transfer
2009/7/30
Primordial germ cells (PGCs) of white leghorn Maria line hens embryos as a donor were transferred white leghorn Laura line hens embryos as a recipient. During stage 12 to 15 of incubating fertilized ...
Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants
chicken cryoprotectants PGCs recovery-rate viability
2009/7/30
Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial...
Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs
chicken chimera primordial germ cell quail soft x-ray
2009/7/29
The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the...
Effects of Busulfan Sustained-release Emulsion on Depletion and Repopulation of Primordial Germ Cells in Early Chicken Embryos
busulfan sustained-release emulsion early chicken embryos germline chimeras primordial germ cells
2009/7/29
The current study aimed to determine the optimal time of busulfan (1,4-butanediol dimethanesulfonate) administration for the preparation of suitable recipient embryos to generate germline chimeras in ...