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Persulfidation-based Modification of Cysteine Desulfhydrase and the NADPH Oxidase RBOHD Controls Guard Cell Abscisic Acid Signaling
Abscisic Acid Signaling NADPH oxidase Persulfidation Guard cells
2024/1/22
Accumulating evidence suggests that hydrogen sulfide (H2S) is a gaseous signaling molecule that regulates diverse cellular signaling pathways through persulfidation, which involves the post translatio...
Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition
reactive oxygen species ram antioxidant
2016/2/23
The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of ...
Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition
reactive oxygen species ram antioxidant
2016/1/29
The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of ...
Negative Cross-Resistance of Acetolactate Synthase Inhibitor–Resistant Kochia (Kochia scoparia) to Protoporphyrinogen Oxidase– and Hydroxyphenylpyruvate Dioxygenase–Inhibiting Herbicides
ALS-inhibitor resistance herbicide resistance pleiotropic effect target-site mutation
2015/12/23
This greenhouse experiment examined the response of homozygous susceptible and acetolactate synthase (ALS) inhibitorresistant plants from six Canadian kochia accessions with the Pro197 or Trp574 mutat...
Saflufenacil (Kixor™): Biokinetic Properties and Mechanism of Selectivity of a New Protoporphyrinogen IX Oxidase Inhibiting Herbicide
Herbicide mechanism of selectivity metabolism uptake and translocation
2015/12/21
Saflufenacil (Kixor™) is a new protoporphyrinogen IX oxidase (PPO) inhibiting herbicide for preplant burndown and selective PRE dicot weed control in multiple crops, including corn. The biokinet...
The Herbicide Saflufenacil (Kixor™) is a New Inhibitor of Protoporphyrinogen IX Oxidase Activity
Herbicide mode of action metabolite profiling physiological profiling
2015/12/21
Saflufenacil (Kixor™) is a new herbicide of the pyrimidinedione chemical class for preplant burndown and selective preemergence dicot weed control in multiple crops, including corn. In this stud...
DISTINCT LOCALIZATION OF NADPH OXIDASE FLAVOCYTOCHROME B IN RESTING AND INTERFERON GAMMA ACTIVATED MACROPHAGES
Host defense superoxide
2015/5/21
Flavocytochrome b558, the catalytic core of the phagocytic NADPH oxidase, mediates the transfer of electrons from NADPH to molecular oxygen to generate superoxide for host defense. Flavocytochrome b i...
Characterisation of polyphenol oxidase and peroxidase and the role in browning of loquat fruit
Eriobotrya japonica PPO POD
2015/3/30
Polyphenol oxidase (PPO) and peroxidase (POD) were extracted from a new loquat fruit cultivar (Ninghaibai) and characterised using reliable spectrophotometric methods. In both cases, the optimum pH fo...
Expression and processing of xanthin dehydrogenase/oxidase cDNA from African cape buffalo
Biological sciences Xanthin dehydrogenase Syncerus caffer Trypanosome Cape buffalo
2015/1/28
Our lab studies oxidative regulation in defense against extracellular pathogens, especially African Trypanosomes. ^ Trypanosomes are protozoan hemoflagellates. The organisms are transmitted by tsetse ...
A Fluorescently Tagged C-Terminal Fragment of p47phox Detects NADPH Oxidase Dynamics during Phagocytosis
NADPH Oxidase Dynamics phagocytosis
2014/12/12
The assembly of cytosolic p47phox and p67phox with flavocytochrome b558 at the membrane is crucial for activating the leukocyte NADPH oxidase that generates superoxide for microbial killing. p47phox a...
FcγR-stimulated activation of the NADPH oxidase: phosphoinositide-binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the phagosome
NADPH oxidase p40phox
2014/12/12
The phagocyte NADPH oxidase generates superoxide for microbial killing, and includes a membrane-bound flavocytochrome b558 and cytosolic p67phox, p47phox, and p40phox subunits that undergo membrane tr...
Rac Activation Induces NADPH Oxidase Activity in Transgenic COSphox Cells and Level of Superoxide Production is Exchange Factor-Dependent
NADPH oxidase Rac
2014/12/12
Transient expression of constitutively active Rac1 derivatives, (G12V) or (Q61L), was sufficient to induce phagocyte NADPH oxidase activity in a COS-7 cell model in which human cDNAs for essential oxi...
Rac GTPase isoform-specific regulation of NADPH oxidase and chemotaxis in murine neutrophils in vivo: Role of the C-terminal polybasic domain
Rac GTPase NADPH oxidase
2014/12/12
The Rho family GTPase Rac acts as a molecular switch for signal transduction to regulate various cellular functions. Mice deficient in the hematopoietic-specific Rac2 isoform exhibit agonist-specific ...
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcγIIA receptor–induced phagocytosis
NADPH oxidase phagocytosis
2014/12/12
Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67phox, p47phox, an...
Characterisation of polyphenol oxidase from Melissa officinalis L. subsp. officinalis (lemon balm)
lemon balm protein enzyme kinetics inhibition
2014/2/24
Polyphenol oxidase (PPO) from Melissa officinalis L. subsp. officinalis (lemon balm) was partially purified by ammonium sulphate precipitation and dialysis; and then it was characterised in detail in ...