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Callus induction and frond regeneration in Spirodela polyrhiza
duckweed regeneration tissue culture
2016/12/8
Spirodela polyrhiza belongs to the family Lemnaceae (duckweed), which is a group of small aquatic plants offering an attractive plant expression system for the production of recombinant protein. No fr...
Effect of different plant growth regulators on callus induction in Catalpa bungei
Catalpa bungei tissue culture plant growth regulators callus
2011/1/12
The present study describes a reliable method for callus induction of Catalpa bungei plant. Calli were in vitro initiated from leaves, stem and petiole segments using DKW basal media. The effect of co...
High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species
Tylophora indica leaf callus somatic embryogenesis shoot organogenesis
2010/3/17
An efficient and reproducible protocol has been developed for the in vitro production of an endangered medicinal climber Tylophora indica (Burm.f.) Merr. via leaf explants. Different types of calli pr...
Callus induction, biomass growth, and plant regeneration in Digitalis lanata Ehrh.: influence of plant growth regulators and carbohydrates
Callus regeneration carbohydrates nitrogen source rooting
2010/3/17
The effect of plant growth regulators (PGRs) and carbohydrate sources on callus induction, callus growth, and plant regeneration in foxglove was examined. Explants were transferred onto MS medium with...
Quantitative Determination of Magnolol in the Callus from Petioles and Mature Seeds of Magnolia obovata
callus Magnolia obovata magnolol neolignan
2009/11/23
The bark of Magnolia obovata has been used as a source of traditional Japanese herbal medication because it contains the medicinal ingredient such as magnolol, which is a kind of neolignan. In this st...
Effects of Complex Organic Extracts on Callus Growth and PLB Regeneration through Embryogenesis in the Doritaenopsis Orchid
potato extract corn extract papaya extract
2009/9/10
Potato extract (PE),corn extract (CE) and papaya extract (PAE) at concentrations of 25, 50 and 100 mL L–1enhanced callus growth on New Phalaenopsis (NP) medium containing sucrose. Among the various co...
In vitro Selection of Soybean Callus Resistant to Fusarium oxysporum Metabolites
In vitro selection disease resistance Fusarium oxysporum Glycine max L.
2010/11/15
Soy bean (Glycine max L.) callus line tolerant to 9% Fusarium oxysporum metabolite was
selected. Initiation and maintenance of callus as well as selection process was carried out on Murashige and Sko...
Cyto-histological Studies on Callus Formation and Its Regeneration in Anther Culture of Oryza satiz}a L
Anther culture Callus Oryza sativa Plant regeneration Rice Ultrastructure
2009/8/6
Histological characteristics of globular shaped pollen callus (globules) developed from multicellular pollen grains were examined in rice anther culture (Oryza sativa L.). The ultrastructure of polle...
A Scanning Electron Microscope Study on Japonica Type Rice Callus Cultures, with Emphasis on Plantlet Initiation
Callus Japonica type rice Membranous layer Nodular structure Oryza sativa Scanning electron microscopy Shoot-bud formation Surface stabilization
2009/8/6
Morphological details of rice callus cultures were examined at the initial stage of plantlet development, together with the growth of callus established from mature seeds, with the aid of scanning elc...
Ultrastructure of Inoculated Anthers in Relation to the Frequency of Induction of Pollen Callus in Anther Culture of Oryza sativa L
Anther Culture Oryza sativa L Rice Ultrastructure
2009/8/6
The formation of pollen callus was induced when rice anthers, which contained microspores in the mid-uninucleate and late-uninucleate stage, were cultured. The ultrastructure of the microspores was al...
Re-Observation with Light Microscope of Scanning Electron Microscopic Specimens in Rice Callus Cultures
Oryza sativa Scanning electron microscopy
2009/8/6
The scanning electron microscopy-light microscopy method (SEM-LM), routinely prepared SEM specimens can be reprocessed for light microscopy (LM), is presented. In this method, specimens were embedded ...
Isolation and Culture of Protoplast from Finger Millet(Eleusine coracana)Callus
Cell division Cell wall Finger millet Protoplast isolation and culture
2009/8/5
High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl-1 2, 4-D and 0.1 mgl-1 kinetin. The protoplasts were release...
Ultrastructure of Electrofused Products from Pansy (Viola tricolor) Mesophyll and Wild Viola (V. patrinii) Petiole Callus Protoplasts
Electrofusion Pansy Protoplast Ultrastructure Wild viola
2009/8/5
Ultrastructural observations were undertaken to study the viability of fusion products by electrofusing the protoplasts from the mesophyll cells of pansy leaves and the cultured callus cells of wild v...
Regeneration via Somatic Embryogenesis and/or Organogenesis in Callus from Mature Seeds of Echinochloa oryzicola Vasing
Barnyard grass Callus formation Organogenesis Regeneration Somatic embryogenesis
2009/8/4
Callus was initiated from mature seeds without the rinds of barnyard grass, Echinochloa oryzicola Vasing, on a modified Murashige and Skoog (MS) medium containing 2.0∼20.0 mgl-1 2, 4-D. The rate...
An Efficient Method for Callus Formation of Protoplasts from Peanut (Arachis hypogaea L.) Leaf Mesophyll
Callus formation Mesophyll protoplast Peanut Protoplast culture
2009/7/30
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0....