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冬青卫矛内生放线菌Streptomyces flavofuscus G1 发酵液中抑菌活性成分研究
冬青卫矛 内生菌 Streptomyces flavofuscus G1 抑菌活性
2016/3/18
从冬青卫矛内生放线菌Streptomyces flavofuscus G1的发酵液中分离得到化合物G13和G19。经核磁共振波谱及质谱分析,并结合相关文献,两个化合物分别被鉴定为phencomycin(G13)和4'-deacetyl-(-)-griseusin A(G19)。抑菌活性测定结果表明: G19对水稻白叶枯病菌Xanthomonas oryzae pv. oryzae、铜绿假单孢菌Ps...
Production, Purification and Characterization of Inulinase from a Newly Isolated Streptomyces sp. CP01
Characterization Inulinase Production Purification Streptomyces
2013/3/8
Inulinase is an enzyme catalyzing the hydrolysis of inulin, a plant reserve polysaccharide, into fructoses and fructooligosaccharides which are widely used as food additives. Here we report inulinase ...
Fractionation and structural identification of antibiotic activity substances from Streptomyces herbaricolor HNS2-2
Streptomyces Herbaricolor HNS2-2 Quercetin Dehydrate
2013/2/28
Four groups of antibiotic activity compounds were separated and purified from the ethyl acetate fraction of a broth culture of Streptomyces herbaricolor HNS2-2.Trace analyses were performed and anti-m...
吸水链霉菌(Streptomyces hygroscopicus)ATCC29253 能够产生非常丰富的次级代谢产物，除了雷帕霉素外，还可以分泌尼日利亚菌素、洋橄榄叶素、六烯类抗生素等多种具有生物活性的物质，具有重要的研究价值和应用前景。【目的】而建立高效的遗传操作系统是研究该链霉菌相关代谢产物合成机理和构建基因工程菌株的基础。【方法】我们测试了不同培养基及供体菌对吸水链霉菌及其它链霉菌接合转移效...
Evaluation of the toxicity of Streptomyces aburaviensis (R9) extract towards various agricultural pests
Algae Catfish Cyanobacteria Fungi Pathogens Streptomyces
2013/3/5
The dichloromethane extract of culture filtrate from Streptomyces aburaviensis R9 was evaluated using various rapid bioassays to determine potential inhibitory effects towards phytopathogenic fungi (C...
Optimization and production of antifungal hydrolysis enzymes by streptomyces aureofaciens against Colletotrichum gloeosporioides of mango
Antifungal Colletotrichum Gloeosporioides
2013/3/4
We isolated naturally occurring actinomycetes with an ability to produce metabolites having antifungal property against, Colletotrichum gloeosporioides, the causal agent of mango anthracnose. One prom...
Evaluation of Streptomyces as a Probiotic Feed for the Growth of Ornamental Fish Xiphophorus helleri
probiotics Actinobacteria Streptomyces ornamental fish Xiphophorus helleri
2011/2/24
The potential of Streptomyces as a probiotic feed for the growth of ornamental fish Xiphophorus helleri has been investigated. The Streptomyces strains used as probiotics were isolated from the marine...
从1株来源于中国南海沉积环境的海洋链霉菌SCSIO 1934的发酵产物中分离鉴定次生代谢产物。 方法 :对海洋链霉菌SCSIO 1934的发酵液进行有机溶剂萃取,利用硅胶、凝胶柱色谱等方法分离次生代谢产物,通过核磁数据和理化性质对各单体化合物进行结构鉴定。 结果 :从菌株Streptomyces sp. SCSIO 1934中分离纯化得到17-脱甲基格尔德霉素(17-O-demethylgelda...
从山东省济南市南部山区土壤中分离得到1株具有广谱抗真菌活性的链霉菌SD07菌株。根据形态特征、生理生化反应和16S rDNA序列分析结果，链霉菌SD07为定为纺锤链霉菌(Streptomyces netropsis)。分离纯化了SD07?产生的抗真菌活性成分并获得其结晶CASD07。抗真菌活性检测结果发现： CASD07对酵母菌、担子菌、接合菌等多种真菌具有广谱抗菌作用。对CASD07...
甾体化合物——LⅣ.化合物 S 醋酸酯经 Streptomyces roseochromogenus 的转化。
对海洋放线菌Streptomyces sp.发酵液的提取物进行柱层析分离, 得到5个化合物. 经MS, NMR, 1H-1H COSY, HSQC和HMBC等数据鉴定, 其结构分别为3-氨基-丙酸对甲苯酯(1)、6-Amino-3-(4-hydroxybenzyl)-1,4-diazonane-2,5-dione(2)、正丁基-α-D-吡喃甘露糖苷(3)、大豆苷元(4)和1H-3-吲哚甲酸(5)....
研究了添加羟胺对Streptomyces hygroscopicus合成谷氨酰胺转胺酶的影响. 结果表明，添加羟胺可诱导谷氨酰胺转胺酶的合成. 摇瓶培养过程中，羟胺的最适添加量和最适添加时间分别为0.5 mmol/L和38 h，发酵结束时，谷氨酰胺转胺酶酶活最高达到5.3 U/mL，比对照提高了32.5%. 添加羟胺可诱导Streptomyces hygroscopicus产酶，羟胺与谷氨酰胺转胺...
通过设计简并引物和构建基因组文库的方法从链霉菌Streptomyces sp.S9中克隆得到β-l,4-木聚糖酶基因xynBS9。该基因全长1 023 bp，编码340个氨基酸。将不带原基因信号肽编码序列的xynBS9以正确阅读框架克隆到表达载体pET-22b(+)上，并在大肠杆菌BL2l(DE3)中诱导表达。重组蛋白经硫酸铵分级沉淀和疏水柱纯化后达到电泳纯。酶学性质分析表明，重组木聚糖酶最适温度...
Regulatory Elements in Tetracycline-Encoding Gene Clusters: the otcG Gene Positively Regulates the Production of Oxytetracycline in Streptomyces rimosus
type II polyketide synthase oxytetracycline biosynthesis Streptomyces rimosus
2010/2/1
The expression of bacterial polyketide synthase gene clusters is often controlled by a number of different families of regulatory proteins that can have either a pathway-specific or a pleiotropic mode...
Aminopeptidases in Mycelium and Growth Medium of Streptomyces rimosus Strains
prolyl leucyl and arginyl aminopeptidases localization
2010/2/1
Aminopeptidases (APs) of the same substrate specificities have been detected in the mycelia and culture filtrate of Streptomyces rimosus. To compare extracellular and intracellular prolyl, leucyl and ...