搜索结果: 1-8 共查到“Pyk2”相关记录8条 . 查询时间(0.125 秒)
探讨外源性生长因子-β1与外源性血小板衍生生长因子BB联合应用对牙周组织破骨细胞内Pyk2表达的影响。方法 160只SD大鼠随机分为2组,建立正畸牙移动模型。A组注射含rhTGF-β1与rhPDGF-BB的混合液 0.1 mL,B组注射相同容量的PBS。加力后1、4、7、10、14 d,分别处死每大组的1小组大鼠。测量牙移动距离变化,TRAP染色行破骨细胞计数,Pyk2蛋白和基因水平分别用免疫组织...
Dynamin and PTP-PEST cooperatively regulate Pyk2 dephosphorylation in osteoclasts
signaling Tyrosine kinase Osteoclasts
2014/11/6
Bone loss is caused by the dysregulated activity of osteoclasts which degrade the extracellular bone matrix. The tyrosine kinase Pyk2 is highly expressed in osteoclasts, and mice lacking Pyk2 exhibit ...
Megakaryocytes Regulate Expression of Pyk2 Isoforms and Caspase-mediated Cleavage of Actin in Osteoblasts
actin bone osteoblasts
2014/11/6
The proliferation and differentiation of osteoblast (OB) precursors are essential for elaborating the bone-forming activity of mature OBs. However, the mechanisms regulating OB proliferation and funct...
Signaling via the Pyk2-Src-Cbl complex downstream of integrins contributes to the assembly, organization, and dynamics of podosomes, which are the transient adhesion complexes of highly motile cells s...
Defective microtubule-dependent podosome organization in osteoclasts leads to increased bone density in Pyk2W22;/W22; mice
podosome organization bone density osteoclast functions
2014/11/6
The protein tyrosine kinase Pyk2 is highly expressed in osteoclasts, where it is primarily localized in podosomes. Deletion of Pyk2 in mice leads to mild osteopetrosis due to impairment in osteoclast ...
Pyk2对人类结肠癌细胞肝转移能力及超微结构的影响
黏着斑激酶2 结肠肿瘤 肝转移 超微结构
2012/2/20
目的： 研究在裸鼠结肠癌肝转移模型中，富含脯氨酸的蛋白酪氨酸激酶2（proline-rich tyrosine kinase 2，Pyk2）对人结肠癌细胞肝转移能力和超微结构的影响。方法：利用正义及反义cDNA技术，在人结肠癌细胞系SW480基础上，构建Pyk2高表达的细胞系SW480-Pyk2+及低表达的细胞系SW480-Pyk2-作为实验组，并以转染空载体的细胞系SW480-vector和...
PYK2靶向短发夹RNA干扰重组体的构建及鉴定
RNA干扰 心脏 基因 PYK2
2009/8/27
目的:本研究利用RNA干扰技术，以PYK2为靶基因，设计构建重组体，并对重组体进行鉴定，为下一步探索心肌纤维化机制的新途径奠定基础。方法:设计有小发夹结构的8条DNA序列，经退火成互补4对双链，再克隆到载体PAVU6＋27中构建重组体，转化入JM109菌株，提取质粒进行酶切鉴定、测序分析。结果:酶切鉴定和测序分析均提示PYK2靶向RNA干扰重组体的构建成功。结论:PYK2靶向RNA干扰重组体的成功...