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Cloning and Expression of Yak Active Chymosin in Pichia pastoris
Active Chymosin Yak Expression Pichia pastoris
2016/11/10
Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain c...
Expression and Purification of Human Lysosomal β-galactosidase from Pichia Pastoris
human β-galactosidase lysosomal storage diseases Pichia pastoris protein expression protein purification biochemistry and molecular biology
2014/12/12
Lysosomal storage diseases are genetically inherited diseases caused by the dysfunction of lysosomal enzymes. In a normal cell, lysosomal enzymes cleave specific macromolecules as they are transported...
巴斯德毕赤酵母(Pichia pastoris)高效异源表达脂肪酶研究进展。
Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastoris Expression System
Pichia pastoris Purification Secretory Expression Tibetan Sheep
2016/5/16
A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific...
Antibacterial Activity of Recombinant Pig Intestinal Parasite Cecropin P4 Peptide Secreted from Pichia pastoris
Cecropin Ascars suum Pichia pastoris Methanol Antimicrobial Activity
2016/5/16
Cecropins (Cec) are antibacterial peptides and their expression is induced in a pig intestinal parasite Ascaris suum by bacterial infection. To explore the usefulness of its activity as an antibiotic,...
Heterologous Expression of Xylanase II from Aspergillus usamii in Pichia pastoris
Aspergillus usamii characterization expression xylanase Pichia pastoris
2009/6/24
To efficiently produce xylanase for food processing industry, a gene encoding xylanase II (XynII) from Aspergillus usamii has been cloned into the vector pPIC9K and integrated into the genome of Pichi...
Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent
tannase Pichia pastoris recombinant protein production
2009/2/25
Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of As...
人可溶性gp190在酵母Pichia pastoris中的表达
毕赤酵母 白血病抑制因子受体 可溶性受体 分泌表达
2008/1/29
摘要 人可溶性gp190(sgp190)是白血病抑制因子(LIF)的可溶性受体,可能在LIF行使众多生物学功能中扮演着重要的角色。为获得这一蛋白以研究它在人体内的生物学活性,在酵母Pichia pastoris中实现了重组sgp190分泌表达。利用基因重组技术,将编码人可溶性gp190(LIF受体α亚基gp190胞外区)cDNA克隆到毕赤酵母Pichia pastoris分泌表达载体pPIC9K...
猪IFNα基因在毕赤酵母中的高效分泌表达High Level Secretion Expression of PoIFNαin Pichia pastoris
猪IFNα 巴斯德毕赤酵母 基因重组 分泌表达
2008/1/10
摘要
巴斯德毕赤酵母载体质粒pPICZαA含有强启动子PAOX1和α-MF信号肽序列,构建猪IFNα基因的重组质粒pPICZαA-IFNα,并转入E.coli JM109中,得到转猪IFNα基因工程菌,经酶切鉴定克隆到载体pPICZαA上的外源基因即为猪IFNα基因。通过电击将经SacⅠ酶切后线性化的pPICZαA-IFNα质粒转化到巴斯德毕赤酵母KM71中。SDS-PAGE和Western b...
摘要 为研究组织型基质金属蛋白酶抑制剂 (TIMPs)的分子作用机制 ,探讨了在 Pichia pastoris酵母中高效表达分泌型人组织型基质金属蛋白酶抑制剂 - 1 (TIMP- 1 )的技术路线 ,并对产物性质进行初步研究 .通过 PCR从含有 TIMP- 1基因的 p BS质粒获得了该基因的全长序列 ,构建了 p PIC9/T1表达载体 ,电击法转化酵母 ,通过表型筛选和 PCR鉴定证实了...
摘要 基质金属蛋白酶 - 9( MMP- 9)可促进恶性肿瘤的侵袭、转移 ,并在组织重建、胚胎发育以及伤口愈合等生理过程中发挥重要作用 .为研究这一蛋白的性质 ,并以之为靶标筛选抗肿瘤转移药物 ,在酵母 Pichia pastoris中实现了重组人 MMP- 9蛋白的高效、高活性、分泌表达 .首先用 PCR扩增了 MMP- 9基因编码区 (不含信号肽序列 ) ,经测序证实后 ,将其插入 p PIC...
摘要 前列腺特异膜抗原是一种具有高度前列腺特异性的糖蛋白 ,其表达的增高与肿瘤的术后复发、激素抵抗及较差的预后正相关 ,在前列腺癌的诊断和治疗中有广泛的应用前景 .从前列腺癌组织提取总RNA ,利用RT PCR技术获得PSMA基因的全长序列 ,构建了重组酵母表达载体pPIC3.5K PSMA .电击法转化Pichiapastoris酵母 ,通过表型筛选和PCR鉴定证实该基因已稳定整合入Pichia...
抗菌肽Magainin基因的克隆及其在Pichia pastoris中的表达
抗菌肽 酵母表达 magainin pPIC3
2007/12/21
摘要 用化学合成法合成了以酵母偏爱密码子编码的抗菌肽magainin基因片段 ,合成片段拼接后 ,与pUC19重组 ,获得magainin基因 .Magainin基因与酵母表达载体pPIC3重组 ,构建胞内表达载体pPIC3 Mag ,电击法转化GS115宿主菌 ,经表型筛选和PCR鉴定证实了目的基因已稳定整合入Pichiapastoris酵母基因组中 .阳性克隆用甲醇诱导表达 ,用免疫印迹法确定...
在甲醇酵母Pichia pastoris胞内表达有活性的辣根过氧化物酶
辣根过氧化物酶 毕赤酵母 基因表达
2007/12/19
摘要 为了开辟在甲醇酵母 Pichia pastoris中表达 HRP的新途径 ,将编码成熟 HRP同功酶 C基因克隆到表达载体 p PIK3.5K中 .p PIK3.5KHRP转化 GS1 1 5后 ,用 PCR筛选阳性 P.pastoris重组株 ,并用甲醇进行诱导 . Western印迹杂交分析表明目标蛋白 (约为 38k D)能被天然 HRP的多克隆抗体所识别 ,因此活性辣根过氧化物酶已在...
摘要 用PCR方法从pPAIJ.7中扩增人纤溶酶原激活剂抑制物2型(PAI-2)基因,与pPUC18重组,经限制性内切酶片段分析与核苷酸序列分析,获得全长人PAI-2基因.PAI-2基因与表达载体pPIC9重组,构建受乙醇氧化酶1基因(AOX1)启动子与转录终止区控制的酵母表达质粒,转化GS115宿主菌,经表型筛选和PCR扩增筛选阳性克隆,用甲醇诱导表达,重组PAI-2以分泌型表达,占分泌总蛋白的...