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Preparation of Epithelial and Stromal Cell Fractions from Immature Rat Prostatic Tissue Using Percoll Gradients
prostate androgens testosterone
2009/2/19
A method is described for the dispersion, isolation, and partial characterization of epithelial and stromal tissue cells from the rat ventral prostate. Viable epithelial and stromal cells have bee...
Sperm function and choice of preparation media：comparison of Percoll and Accudenz discontinuous density gradients
Sperm maturity and creatine kinase motile sperm yield sperm activation chiortetracycline
2009/2/16
We compared the sperm populations prepared by Accudenz (35-65%) and Percoll (40-80%) density gradients in 21 normospermic specimens (concentration, 53.6 +/- 3.8 x 10(6) sperm/ml; motility, 44.5 +/...
Separation of cryopreserved human semen using Sephadex columns, washing, or Percoll gradients
Sperm separation intrauterine insemination seminal plasma sublethal damage
2009/2/7
The following methods were evaluated for their ability to separate motile cryopreserved sperm from semen after thawing: single washing, Percoll separation followed by a single washing, and Sephad...
Characterization of functional Leydig cells after purification on a continuous gradient of percoll
functional Leydig cells purification continuous gradient of percoll
2009/2/6
Two human chorionic gonadotropin (hCG) responsive cells from rat testicular interstitium were previously isolated on a discontinuous gradient of Percoll. The light cells were non-steroidogenic and...
Marked Differences in Protamine Content and P1/P2 Ratios in Sperm Cells From Percoll Fractions Between Patients and Controls
Chromatin condensation male infertility Percoll gradients sperm chromatin sperm morphology sperm motility
2008/12/19
The various sperm cell types present in a simple ejaculate differ in their motility and morphology. However, little is known about the nuclear maturity of these sperm cells and their relationship wi...
Percoll密度梯度离心分离不同发育阶段生精细胞的方法1)
Percoll 密度梯度离心分离 发育阶段 生精细胞
2008/1/17
精子发生过程是生命周期中的重要过程，也是研究高等动物细胞分化的一个独特体系。由于精子发生这一连续过程是在皋丸组织中完成的，所以不同发育阶段的生精细胞彼此共存，而要进行有关的生化研究，必须得到均一的处于某一特定发育阶段的生精细胞。因此，如何从翠丸组织中分离出特定发育时期的生精细胞，是进行深人研究的首要条件。
应用percoll密度梯度离心分离不同类型的花粉粒
2008/1/15
密度梯度离心方法是纯化生物大分子、分
离细胞和细胞亚结构（核、叶绿体、线粒体）以及
病毒的一种有用技术，在细胞生物学和分子生
物学的研究中，它起了很重要的作用。曾经使
用过的密度梯度离心介质〔如蔗糖、血清白蛋
白、Ficoll（聚蔗糖）等I，都不是理想的介质。近
年来新发展的梯度禽心介质— percoll是目前
理想的介质。它具有渗透性低、不穿透细胞、粘
度低、密度高和无毒害等优点...