搜索结果: 1-15 共查到“GFP”相关记录71条 . 查询时间(0.058 秒)
携带GFP的MNSV侵染性克隆载体的构建
melon melon necrotic spot virus infectious clone recombination vector pathogenicity
2020/10/30
Melon necrotic spot virus (MNSV) is a newly reported melon-infecting virus species in China, which is seriously terrible in melon production. The MNSV full-length cDNA of MNSV was obtained by RT-PCR a...
由大丽轮枝菌引起的棉花黄萎病是一种难以防治的土传病害,西兰花残体还对其有一定的防治作用,防效为62.82%。为解析西兰花残体对棉花黄萎病的防治机制,本研究通过土壤杆菌转化的方法构建了大丽轮枝菌的绿色荧光标记菌株,采用共聚焦显微镜观察标记菌株在添加西兰花残体营养基质中和空白对照营养基质种植的棉花体内的侵染和扩展情况。结果表明构建的标记菌株gfp-wx-1与野生菌株wx-1的生物学特性无显著性差异。同...
[目的]旨在建立农杆菌介导的绿色荧光蛋白（GFP）基因转化钩状木霉（Trichoderma hamatum）ACCC31649的技术方法，筛选遗传稳定的GFP标记转化子，并研究该菌株在辣椒植株中定殖和对辣椒的促生作用，为进一步阐明木霉在辣椒根际定殖及其与辣椒病原菌在辣椒根际和植株中的定殖、互作和生防作用奠定基础。[方法]通过根癌农杆菌介导的遗传转化方法筛选遗传稳定的GFP标记转化子，通过灌根接种方...
利用绿色荧光蛋白GFP研究稻瘟病菌与水稻的互作
稻瘟病菌 绿色荧光蛋白 侵染过程 水稻
2017/11/28
稻瘟病菌Magnaporthe oryzae严重威胁水稻的产量与质量,明确稻瘟病菌与水稻互作过程及机理,对防治稻瘟病具有重要意义。本研究利用稻瘟病菌常用致病菌株GUY11和ZB25, 构建了绿色荧光蛋白GFP的过量表达菌株,并通过荧光显微观察菌株侵染寄主水稻过程中侵染结构的形成与发育,包括孢子萌发、附着胞形成、侵染钉形成、侵染菌丝增殖、坏死斑形成及产孢。另外,通过比较过量表达菌株对稻瘟病高抗水稻和...
Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP)
Short Hydrogen Bonds Proton Delocalization Green Fluorescent Protein GFP
2016/5/25
Short hydrogen bonds and specifically low-barrier hydrogen bonds (LBHBs) have been the focus of much attention and controversy for their possible role in enzymatic catalysis. The green fluorescent pro...
Ground-state Proton Transfer Kinetics in Green Fluorescent Protein (GFP)
Mitotic centromere-associated kinase (MCAK/Kif2C) Cellular senescence p53 Human primary cells
2016/5/25
Proton transfer plays an important role in the optical properties of green fluorescent protein (GFP). While much is known about excited-state proton transfer reactions (ESPT) in GFP occurring on ultra...
GFP Variants with Alternative β‑Strands and Their Application as Light-driven Protease Sensors:A Tale of Two Tails
Green Fluorescent Proteins Peptide Hydrolases Light
2016/5/25
Green fluorescent protein (GFP) variants that carry one extra strand 10 (s10) were created and characterized, and their possible applications were explored. These proteins can fold with either one or ...
Thermodynamics,Kinetics,and Photochemistry of Beta-Strand Association and Dissociation in a Split-GFP System
Green Fluorescent Proteins Protein Structure,Secondary Kinetics Photochemistry Thermodynamics Models, Molecular
2016/5/25
Truncated green fluorescent protein (GFP) that is refolded after removing the 10th β-strand can readily bind to a synthetic strand to recover the absorbance and fluorescence of the whole protein. This...
The Electronic Structure of the Chromophore in Green Fluorescent Protein (GFP)
Electronic Structure Chromophore Green Fluorescent Protein GFP
2016/5/23
The chromophore responsible for the fluorescence from Green Fluorescent Protein (GFP) of the jelly fish Aequorea Victoria1,2 is formed in an autocatalytic, posttranslational cyclization and oxidation ...
Development and Characterization of Caspase Activatable GFP and a Family of Fluorescent Reporters
apoptosis GFP
2014/11/20
The cellular process of programmed cell death, or apoptosis, is critical in homeostasis and development. In addition it's misfunction is implicated in an array of disease states from cancer to neurode...
恒磁场对Feridex-GFP 双标记BMSC 在损伤肝脏中定植的影响
移植 Feridex-GFP双标记 恒磁场 定植
2014/11/3
评价体外恒磁场作用下，双标记干细胞经外周静脉、门静脉、肝动脉移植在损伤肝脏中的定植率及治疗急性肝损伤的有效性。方法：分离骨髓间充质干细胞(bone marrow mesenchymal stem cells，BMSCs)，经培养传代纯化、体外诱导，分化为肝样细胞，绿色荧光蛋白(green fluorescent protein，GFP)和菲立磁(Feridex)体外双标记BMSCs，不同途径移植双...
基于GFP的锌指核酸酶真核细胞筛选体系的构建与验证
锌指核酸酶 筛选通用载体 绿色荧光蛋白 真核细胞
2014/7/22
旨在构建基于GFP报告基因的ZFN真核细胞筛选体系，为ZFN的筛选提供一种直观、准确、灵敏的方法。以pEGFP-N1为基础，去除EGFP基因的起始密码子后在多克隆位点上游插入ATG，构建筛选体系的通用载体pEGFP-ATG。将ZFN的靶序列插入到通用载体EGFP基因上游，且使起始密码子和EGFP基因处于不同ORF中，构建筛选载体pEGFP-728。将pEGFP-728转入Hela细胞中，G418筛...
GFP-LC3真核表达载体构建、定位及胶质瘤U87稳定转染细胞系筛选
绿色荧光蛋白质类 自噬 神经胶质瘤 LC3
2014/2/24
构建GFP-LC3真核表达载体，验证其在胶质瘤U87细胞系中对细胞自噬现象的指示作用，并获得稳定转染该载体的U87细胞。方法 以人外周血cDNA为模板扩增全长LC3基因，通过分子生物学操作将其导入pcDNA3-GFP中，得到重组质粒pcDNA3-GFP-LC3。将重组及对照质粒转染胶质瘤U87细胞系，雷帕霉素(Rapamycin)处理48 h后用Western blot方法检测细胞中自噬相关蛋白表...
Transient GUS and GFP Expression in Spanish Red Cedar (Cedrela odorata L.) Somatic Embryos. Optimization of Bombardment Conditions and Evaluation of Selective Agent Lethal Dose
Biolistics Genetic Transformation Tree Genetic Modification Tropical Wood
2013/3/4
Cedrela odorata is a tropical tree widely appreciated for its wood. Commercial plantations are frequently hampered by the attack of the meliacea borer, Hypsipyla grandella, and the lack of resistant v...
SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA添加PolyA尾的DNA序列(240 bp), 含有AATAAA六核苷酸多腺苷化信号(Polyadenylation signal)。在pEGFP-C1质粒的GFP基因下游插入14个同向串联的Alu序列(Alu14), 构建pAlu14质粒, 瞬时转染HeLa细胞, 用Northern blot检测...