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Synthesis of a Thymidine Phosphoramidite Labelled with 13C at C6: Relaxation Studies of the Loop Region in a 13C Labelled DNA Hairpin
Thymidine Phosphoramidite Labelled 13C C6 Relaxation Studies 13C Labelled DNA Hairpin
2016/5/20
A thymidine phosphoramidite labelled at C6 with 13C has been synthesized, and incorporated into a synthetic oligonucleotide, d(CGCGT*T*GT*T*CGCG), which adopts a hairpin conformation. NMR relaxation m...
肝癌组织中线粒体DNA D-Loop区碱基变异与ROS水平Mutations in the D-Loop Region of Mitochondrial DNA and the ROS Level in the Tissue of Hepatocellular Carcinoma
线粒体DNA D-Loop 原发性肝癌 突变 活性氧类物质
2008/1/6
摘要
为了探讨ROS水平与突变的关系，对原发性肝癌线粒体DNA区的突变情况进行研究，同时对原发性肝癌患者组织细胞内ROS进行测定。选择20例原发性肝癌组织及其邻近的癌旁组织，用PCR方法将线粒体DNA D-Loop扩增后测序。组织内ROS的水平采用流式细胞技术测定。结果表明在20对原发性肝癌组织中存在8对mtDNA突变，突变率为40%,共发现突变位点53个，包括2个插入，11个缺失...
云南保山猪线粒体DNA D-loop区序列初步分析Primary Analysis of Mitochondrial DNA D-loop Region Sequence in Baoshan Pig
保山猪 线粒体DNA D-loop 高变区I 遗传多样性
2007/12/31
摘要
摘要：为了解云南保山猪(Baoshan pig)的遗传多样性及其遗传背景，我们测定了19个个体线粒体DNA D-loop高变区I 15 363～15 801片段序列438 bp。检测到10种单倍型，包括8个多态位点，其中5次T/C转换、1次G/A转换、1次G/C颠换和1次A/T颠换，其A、T、G、C碱基的平均含量分别为35.4%、26.9%、13.2%和24.5%，A+T含量(62.3%)...
Genetic Diversity and Phylogenetic Analysis of the mtDNA D-loop Region in Tibetan Sheep
mtDNA D-loop Maternal Sources Tibetan Sheep Genetic Diversity
2016/4/25
Seventeen haplotypes were detected from the complete mitochondrial DNA control region sequences analyzed from eighty individuals of two Tibetan domestic sheep breeds. The nucleotide composition of all...
Genetic Relationships of Cattle Breeds Assessed by PCR-RFLP of the Bovine Mitochondrial DNA D-loop Region
Bos Taurus Bos Indicus Mitochondrial DNA PCR-RFLP Genetic Distance
2016/4/20
To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asi...