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浙江大学医学院段树民院士团队Cell Research发文报道溶酶体蛋白酶cathepsin D具有pH及构象依赖的双重酶活性（图）
浙江大学医学院 段树民院士 Cell Research 溶酶体蛋白酶 cathepsin D pH 构象依赖 双重酶活性
2021/2/4
溶酶体中存在多种负责降解多肽或蛋白质的蛋白水解酶。为确保它们在特定部位与环境发挥催化作用、避免“滥杀无辜”，蛋白水解酶通常以酶原的形式在囊泡内大量储存。在传统认识中，酶原是不具有催化活性的前体蛋白。然而，酶原在功能上真的只是“沉默的大多数”吗？
目前关于组织蛋白酶（cathepsin）在猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）生活周期中发挥功能的研究较少。为了探究宿主细胞中cathepsin在PRRSV吸附、入侵、脱壳、生物合成、组装以及释放过程中的作用，选取源于MA-104的非洲绿猴胚胎肾上皮细胞Marc145细胞系（对PRRSV易感）；...
STRUCTURAL BIOLOGY AND PHARMACOLOGY OF HUMAN CATHEPSIN A AND NEURAMINIDASE 1
Cathepsin A Neuraminidase Lysosomal storage diseases sialidosis Galactosialidosis serine carboxypeptidase
2014/12/10
Human cathepsin A (also known as Protective Protein/Cathepsin A, PPCA; E.C. 3.4.16.5) is a lysosomal serine carboxypeptidase. Cathepsin A is also involved in a complex with two other lysosomal enzymes...
组织蛋白酶X（Cathepsin X, Cat X）是最近发现的一种组织蛋白酶（Cathepsins, Cats）家族成员。近年来研究表明Cat X与多种恶性肿瘤发生、发展有关。本研究旨在探讨肺癌患者血清Cat X及cystatin C的表达与临床特征及预后的关系。方法 采用ELISA法定量检测84例肺癌患者及36例健康对照者血清Cat X及cystatin C表达。结果 肺癌患者血清Cat X和...
Immunoexpression of Cathepsin D and S100A4 Protein and Their Molecular Subtyptes in Canine Mammary Carcinomas
Cathepsin Mammary Tumors Metastasis-Associated Proteins Molecular Subtypes S100 Protein
2013/2/19
Cathepsin D (CD), a lysosomal protease, and S100A4 protein, a calcium binding motif, are considered to be involved in metastasis in various human cancers. No data regarding such proteins are available...
探讨caveolin-1和cathepsin D蛋白在非小细胞肺癌(non-small cell lung carcinoma,NSCLC)中的表达及其与微血管密度(microvessel density,MVD)及临床病理特征之间的关系。方法用免疫组织化学法检测caveolin-1和cathepsin D在86例NSCLC和20例癌旁肺组织中的表达,以及CD34在NSCLC中的表达,并对MVD计...
Cathepsin B反义RNA抑制乳腺癌细胞的侵袭和迁移
反义RNA 转染 黏附 侵袭
2012/5/18
探讨人cathepsin B(CatB)反义RNA在乳腺癌细胞侵袭与迁移中的作用。方法: 构建携CatB反义RNA的重组质粒pBudCE4.1-antiCatB，采用脂质体法将重组质粒瞬时转染人乳腺癌细胞MDA-MB-231。Western blotting法检测MDA-MB-231细胞中CatB蛋白的表达, MTT法检测MDA-MB-231细胞的增殖，细胞-基质黏附实验检测反义CatB对MDA-...
Cathepsin B反义RNA抑制乳腺癌细胞的侵袭和迁移
cathepsin B 基因 反义RNA 转染 黏附 侵袭
2014/4/9
探讨人cathepsin B(CatB)反义RNA在乳腺癌细胞侵袭与迁移中的作用。方法: 构建携CatB反义RNA的重组质粒pBudCE4.1-antiCatB，采用脂质体法将重组质粒瞬时转染人乳腺癌细胞MDA-MB-231。Western blotting法检测MDA-MB-231细胞中CatB蛋白的表达, MTT法检测MDA-MB-231细胞的增殖，细胞-基质黏附实验检测反义CatB对MDA-...
【目的】研究猪肉嫩度性状候选基因-组织蛋白酶B（cathepsin B,CTSB）和半胱氨酸蛋白酶抑制素B（cystatin B,CSTB）在体内不同组织、不同发育阶段的表达变化规律,为研究嫩度性状的遗传调控机理提供依据。【方法】采用荧光探针RT-PCR,定量分析CTSB和CSTB mRNA在两个品种猪的多个组织、多个发育时间点的表达量。【结果】RT-PCR结果表明,CTSB和CSTB mRNA表...
Immunohistochemical Expression of C-Erb-B2, Steroid Receptors and Cathepsin-D in Breast Carcinomas: A Clinicopathologic Trial
Breast carcioma cathepsin-D c-erb-B2
2009/6/29
Aim: We investigated the role of c-erb-B2, hormone receptors and cathepsin D in breast carcinoma progression and their correlation with each other. Methods: Forty-six breast carcinoma patients, operat...
【目的】研究猪肉嫩度性状候选基因-组织蛋白酶B（cathepsin B,CTSB）和半胱氨酸蛋白酶抑制素B（cystatin B,CSTB）在体内不同组织、不同发育阶段的表达变化规律,为研究嫩度性状的遗传调控机理提供依据。【方法】采用荧光探针RT-PCR,定量分析CTSB和CSTB mRNA在两个品种猪的多个组织、多个发育时间点的表达量。【结果】RT-PCR结果表明,CTSB和CSTB mRNA表...
Immuno-histochemical characteristics of necrotic area in the old-infarction area of human brain by using of antibodies against cystatin C, Cathepsin B, puromycin sensitive alanyl-aminopeptidase and Prostaglandin D2 synthase - A preliminary examination
Cystatin C Prostaglandin D2 synthase Brain infarction
2009/3/3
Figure 1: Old infarct area in the cerebellum from an individual (autopsy no.0636). Reticulation made by collagen-like fibers exist in the intact cortex by HE stain (1-A, top). Anti CC clearly sta...
Cathepsin L-like cysteine proteases from Brugia malayi：cDNA cloning and comparison with Caenorhabditis elegans
Cathepsin L-like cysteine proteases Brugia malayi cDNA cloning Caenorhabditis elegans
2009/3/3
Proteases are thought to be potentially useful targets for developing medicines to control filariasis caused by parasitic nematodes. To screen cysteine proteases essential for viability of nematodes...
Cathepsin S的表达与消化系肿瘤的关系
组织蛋白酶S 肿瘤 转移 抑制剂
2009/2/8
组织蛋白酶(Cathepsin)在蛋白降解中起重要作用. 组织蛋白酶S(Cathepsin S, Cat S)属半胱氨酸蛋白水解酶, 作为一个单链酶, 没有糖基化位点. 依据细胞类型和细胞微环境, Cat S可在多处分布并发挥功能. 除消化系肿瘤外, 还参与星形细胞瘤、成胶质细胞瘤、前列腺癌、肺癌等肿瘤的发生. Cat S能够降解细胞外基质分子, 参与肿瘤血管生成和血管内外肿瘤细胞的转移. 本文就...
Regulation of Sulfated Glycoprotein-1 and Cathepsin D Expression in Adult Rat Epididymis
Light microscopy orchidectomy ligation hypophysectomy immunocytochemistry
2008/12/19
Endocytosis, whereby proteins are internalized from the epididymal lumen to be eventually degraded in lysosomes, is one of the major functions of the epididymal epithelial cells in maintaining a pro...