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Two-dimensional gel electrophoretic analysis of rat sperm membrane interaction with cauda epididymal fluid
Two-dimensional gel electrophoretic analysis rat sperm membrane interaction cauda epididymal fluid
2009/2/23
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used to analyze the polypeptide composition of rat cauda epididymal fluid, blood serum and membrane-enriched fractions of caput, corp...
The correlation between the gossypol contents in blood plasma, rete testis fluid, and cauda epididymal fluid following chronic treatment with gossypol in rats
gossypol contents blood plasma rete testis fluid cauda epididymal fluid chronic treatment with gossypol in rats
2009/2/23
Concentrations of gossypol in blood plasma, rete testis fluid, and fluid from the caudia epididymidis were measured simultaneously by high performance liquid chromatography in rats treated with goss...
Isolation and characterization of the plasma membrane of rat cauda epididymal spermatozoa
Isolation characterization plasma membrane rat cauda epididymal spermatozoa
2009/2/23
Cauda epididymal rat spermatozoa were isolated by flushing the excised epididymis and the plasma membrane was detached by a nitrogen cavitation treatment (500 psi, 10 minutes equilibration at 4 C)...
Alpha-glucosidase, glycerophosphocholine, and L-carnitine were measured in sperm-free seminal plasma to determine whether these markers reflected the epididymal function of men attending an infert...
Further characterization of a secreted epididymal glycoprotein in mice that binds to sperm tails
Further characterization secreted epididymal glycoprotein mice sperm tails
2009/2/23
Sperm maturation antigen 4 (SMA-4) is a surface component of the mouse sperm tail. Previously, immunofluorescence studies indicated that SMA-4 may be secreted by principal cells of the distal caput ...
Corticosterone-induced changes in serum hormone profiles and the lipid composition of the caput and cauda epididymidis were studied. The analysis was conducted in both unwashed and washed (free from...
Identification and isolation of epididymal luminal proteins of the mouse
Identification isolation epididymal luminal proteins mouse
2009/2/23
Proteins of mouse cauda epididymal fluid were analyzed by polyacrylamide gel electrophoresis. Fluid expressed from the cauda epididymidis and samples obtained by micropuncture of the epididymal lume...
Alpha-glucosidase as a specific epididymal enzyme marker. Its validity for the etiologic diagnosis of azoospermia
azoospermia epididymal marker aglucosidase carnitine obstructive pathology
2009/2/23
Azoospermic semen was obtained from 39 vasectomized men and 93 patients consulting for infertility. The latter underwent bioclinical investigations including measurement of plasma FSH and testicular...
Growth and characterization of polarized monolayers of epididymal epithelial cells and Sertoli cells in dual environment culture chambers
polarized monolayers epididymal epithelial cells Sertoli cells dual environment culture chambers
2009/2/23
Epididymal epithelial cells isolated from mature rats and Sertoli cells isolated from 10-day-old rats were cultured in serum-free defined media on extracellular matrix impregnated filters maintained...
Epididymal fluid macromolecules do not act as auto- or alloantigens
Epididymal fluid macromolecules auto- or alloantigens
2009/2/23
Because epididymal secretory glycoproteins form important functional associations with maturing spermatozoa, the possibility has been explored that epididymal antigens might be useful in contracep...
Immobilin, the highly viscoelastic glycoprotein isolated from rat cauda epididymal fluid, exhibits all of the key biochemical characteristics of a mucin: 1) it has a very high molecular weight (will...
Decreased fertility and motility of spermatozoa from rats immunized with a prealbumin epididymal-specific glycoprotein
acidic epididymal glycoprotein spermatozoa rat immunization fertility
2009/2/23
This study investigated the effects on the progressive motility, zona-binding capacity, and fertility of spermatozoa from the cauda epididymidis of adult male rats that were actively immunized again...
Effect of gossypol acetate on guinea pig epididymal spermatozoa in vivo and their susceptibility to capacitation in vitro
gossypol sperm motility sperm maturation cytoplasmic droplet capacitation acrosome reaction
2009/2/20
To determine the effects of gossypol acetate on guinea pig epididymal and vas deferens sperm maturity and in vivo susceptibility to in vitro capacitation and the acrosome reaction, we examined sperm...
Similar biochemical properties of human seminal plasma and epididymal alpha-1,4-glucosidase
1-4 glucosidase seminal plasma epididymis
2009/2/20
We have studied some characteristics of alpha-1,4-glucosidases in human male reproductive organs in order to obtain information on the origin of the enzyme in seminal plasma. Acid and neutral enzyme...
Organ culture of rat caput epididymal tubules in a perifusion chamber
Organ culture rat caput epididymal tubules perifusion chamber
2009/2/20
We have been able to culture caput epididymal tubules from rats by modifying an organ culture system (Orgebin-Crist et al, 1980) which was used to culture rabbit corpus epididymal tubule segments. T...