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Phosphatidylcholine synthesis in human spermatozoa
Phosphatidylcholine synthesis human spermatozoa
2009/2/23
To clarify the mechanism of phospholipid synthesis in spermatozoa, fresh human spermatozoa were incubated with labeled fatty acids and 1-acyl-lysophosphatidyl choline (LPC) in the presence or abse...
Cytochalasin D inhibits penetration of hamster eggs by guinea pig and human spermatozoa
Cytochalasin D inhibits penetration hamster eggs guinea pig human spermatozoa
2009/2/23
Fertilization experiments using zona-free hamster eggs and spermatozoa from both guinea pig and human were conducted in the presence of cytochalasin D to evaluate the possible role of actin filame...
Apparent fertility of human spermatozoa from the caput epididymidis
Apparent fertility human spermatozoa caput epididymidis
2009/2/23
Fifty-one patients with obstructive azoospermia caused by blockage at the caput epididymidis have been followed for 4 years after undergoing "specific tubule" vasoepididymostomy, bypassing the cor...
A simple, clinical assay to evaluate the acrosin activity of human spermatozoa
acrosin activity human spermatozoa
2009/2/23
Acrosin, a sperm-specific acrosomal proteinase, has an essential role in the fertilization process. Low levels of acrosin appear to be associated with subfertility and infertility, and the acrosin a...
Ethanol accelerates acrosomal loss in human spermatozoa
Ethanol accelerates acrosomal loss human spermatozoa
2009/2/23
The effects of ethanol on the loss of the human sperm acrosome, as determined by the chlortetracycline fluorescence assay and by indirect immunofluorescence assay, were assessed over 6 hours during ...
The relationship between the motility and morphology of spermatozoa in human semen
semen sperm motility sperm morphology male infertility
2009/2/23
High-speed videomicrography was used to assess simultaneously the morphology and motility of seminal spermatozoa from 10 fertile donors and 10 patients being evaluated for infertility. In both don...
Isolation and characterization of the plasma membrane of rat cauda epididymal spermatozoa
Isolation characterization plasma membrane rat cauda epididymal spermatozoa
2009/2/23
Cauda epididymal rat spermatozoa were isolated by flushing the excised epididymis and the plasma membrane was detached by a nitrogen cavitation treatment (500 psi, 10 minutes equilibration at 4 C)...
A microperfusion chamber for study of mammalian spermatozoa
microperfusion chamber mammalian spermatozoa
2009/2/23
The design of a microperfusion chamber is presented for use with spermatozoa or other cell suspensions. This chamber allows perfusion of a small number of spermatozoa during simultaneous observat...
Relationships between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility
computerized measurements motion frozen-thawed bull spermatozoa fertility
2009/2/23
A computerized system (CellSoft, CRYO Resources, Ltd.) was validated using video tapes of frozen-thawed bull spermatozoa diluted in filtered (0.2 micron) egg yolk-citrate extender (8 X 10(6) spermat...
Correlation between defective motility（asthenospermia）and ATP reactivation of demembranated human spermatozoa
Human spermatozoa motility asthenospermia
2009/2/23
Human spermatozoa treated with 0.05% Triton X-100 to remove the cell membranes became immotile but flagellar movement was reinitiated by addition of 0.06 to 1 mM adenosine triphosphate (ATP). The pe...
Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity
Human spermatozoa liquid peroxidation superoxide production hydrogen peroxide production
2009/2/23
Spontaneous lipid peroxidation in washed human spermatozoa was induced by aerobic incubation at 32 C and measured by malonaldehyde production; loss of motility during the incubation was determined s...
Cryosurvival of human spermatozoa frozen in eight different buffer systems
human spermatozoa eight different buffer systems
2009/2/23
The present study was conducted to ascertain optimal cryoconditions for human spermatozoa by comparing the relative cryoprotective efficiency of eight buffer systems and assessing various cryovial...
Nitrocellulose and polyvinyl coatings prevent sperm adhesion to glass without affecting the motility of intact and demembranated human spermatozoa
Nitrocellulose polyvinyl coatings sperm adhesion glass without
2009/2/23
The effectiveness of several glass coating agents in preventing sperm adherence to glass surfaces for both intact and demembranated human spermatozoa was investigated. These agents included bovine s...
Biochemical studies of metalloendoprotease activity in the spermatozoa of three mammalian species
Biochemical studies metalloendoprotease activity spermatozoa three mammalian species
2009/2/23
Ejaculated porcine and human spermatozoa, hamster spermatozoa from the cauda epididymidis, isolated hamster sperm heads and hamster cytoplasmic droplets contained activity that hydrolyzed the metall...
Kinetics of human spermatozoa long-chain fatty acid：CoASH ligase
human spermatozoa long-chain fatty acid CoASH ligase
2009/2/23
The kinetics of long-chain saturated fatty acid activation were studied in the supernatant obtained from Triton-treated human spermatozoa. Sperm long-chain fatty acid: CoASH ligase (AMP) (E.C. 6.2.1...