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Synchronous assay for human sperm capacitation and the acrosome reaction
human sperm capacitation acrosome reaction
2009/2/23
A synchronous acrosome reaction system was established for human spermatozoa. Seminal plasma is removed from the spermatozoa by centrifugation and the washed spermatozoa are capacitated in a modif...
Evidence for a role of cyclooxygenase（prostaglandin synthetase）and prostaglandins in the sperm acrosome reaction and fertilization
spermatozoa fertilization acrosome reaction cyclooxygenase prostaglandin inhibitor
2009/2/23
Three cyclooxygenase (prostaglandin synthetase) inhibitors, indomethacin, phenylbutazone, and oxyphenbutazone, decreased fertilization in vitro when mixed with capacitated mouse spermatozoa before a...
A study of phospholipase in albumin and its role in inducing the acrosome reaction of guinea pig spermatozoa in vitro
capacitation acrosome reaction spermatozoa
2009/2/19
Phospholipase activity associated with guinea pig spermatozoa during capacitation and the acrosome reaction (AR) was determined by measuring the production of [14C] glycerylphosphorylcholine (GPC) f...
The Effect of ATP Depletion upon the Acrosome Reaction in Guinea Pig Sperm
sperm motility ATP depletion acrosome
2009/2/19
The suspension of guinea pig spermatozoa in substrate-free medium results in the cessation of sperm motility and a 94% reduction in adenosine triphosphate (ATP) levels within minutes. ATP depletion wa...
Acrosome reaction in dog sperm is induced by a membrane-localized progesterone receptor
Canine spermatozoa prostatic fluid
2009/2/18
The aim of this study was to investigate whether the dog sperm acrosome reaction can be induced by progesterone and whether the action of progesterone is mediated by binding of progesterone to a r...
Involvement of reactive oxygen species in human sperm arcosome reaction induced by A23187, lysophosphatidylcholine, and biological fluid ultrafiltrates
Hydrogen peroxide catalase superoxide dismutase chemiluminescence
2009/2/18
Although recent evidence indicated that the production of reactive oxygen species (ROS) by human spermatozoa may be involved in the regulation of capacitation, very little is known about the role ...
The human acrosome reaction is highly sensitive to inhibition by cyclodiene insecticides
Sperm fertilization chiordane endosulfan
2009/2/17
The mammalian sperm acrosome reaction (AR) is essential to fertilization. It can be initiated in vitro by progesterone, a putative physiological initiator that helps to activate sperm GABA(A) recept...
Time course of spontaneous in vitro sperm acrosome reaction
Capacitation fertility potential
2009/2/17
The acrosome reaction (AR) is an exocytotic process essential for sperm penetration of the zona pellucida and binding to the oocyte (DeJonge, 1994). Evaluation of in vitro AR can suggest fertility p...
Decreased spermatogenesis as the result of an induced autoimmune reaction directed against the gonadotropin receptors in male rats
Spermatogenesis testis FSHR LHR sperm number autoimmune antireceptor antibody fertility
2009/2/17
The presence of luteinizing hormone (LH) and testosterone is considered critical for the maintenance of spermatogenesis in the rat. However, the role and importance of follicle-stimulating hormone...
Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm
Macaca fascicularis semen capacitation fertilization
2009/2/17
Hyaluronic acid (HA) not only surrounds the zona pellucida as part of the cumulus matrix but also is present throughout the zona pellucida and the perivitelline space of many mammalian oocytes. Ho...
Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction
Nitric oxide synthase acrosome reaction
2009/2/17
A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We com...
Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa
FITC-PNA hemizona acrosome reaction
2009/2/17
Peanut agglutinin (PNA) was used to assess the sperm acrosomal status and the acrosome reaction during gamete interaction in the equine species. PNA exclusively binds to the outer acrosomal membra...
P450 aromatase messenger ribonucleic acid expression in male rat germ cells: detection by reverse transcription-polymerase chain reaction amplification
Germ cells spermatids P450 aromatase estrogen
2009/2/17
We have previously demonstrated that cytochrome P450 aromatase (P450arom) protein, an estrogen-synthesizing enzyme, is present and active in germ cells of the adult mouse testis. To establish that P...
Maturation of monkey spermatozoa in the epididymis with respect to their ability to undergo the acrosome reaction
Capacitation primate development epididymis macaque
2009/2/16
Spermatozoa obtained along the length of the epididymides from five macaque monkeys (Macaca fascicularis) were examined for their ability to undergo the acrosome reaction under basal conditions and ...
Chloride efflux during the progesterone-initiated human sperm acrosome reaction is inhibited by lavendustin A, a tyrosine kinase inhibitor
GABAA receptor Cl- channel
2009/2/16
Previous studies showed that progesterone (P) can initiate the mammalian sperm acrosome reaction (AR) in vitro and that a sperm GABAA-like receptor/Cl- channel is involved in an essential Cl- effl...